Analysis and Prediction of Perfluorane Adsorption of Drugs on the Basis of the Physicochemical Properties of Ligands

Spectral and kinetic characteristics of indotricarbocyanine complexation with albumin

“…The practical application of dyes is based on their interac tion with plasma proteins (human serum albumin (HSA), lipoproteins) to give a complex between the dye and protein, which leads to an increase in its fluo rescence and stabilization of the dye in the complex. Albumin is the most important carrier of many endog enous and exogenous compounds in organism [3][4][5]; therefore, the interaction of dye and albumin is the key stage of fluorophore delivery into retinal blood vessels.…”

“…We consider the measured life time of 0.22 s to characterize the s cis-trans isomerization in center I; i.e., the characteristic first order rate con stant of isomerization is k 1 = 4.5 s -1 . The characteristic life time for center II in subdo main IIIA is considerably longer (9.27 s), while the first order rate constant of isomerization is consider ably shorter k 2 = 1.1 × 10 -1 s -1 , which seems to be caused by relatively small size of pocket of this binding center (0.7 × 1.4 nm) [3][4][5], which is comparable with the size of dye molecule. For center II, the isomeriza tion process typical for cyanine dyes after attachment to biomacromolecule becomes sterically more hin dered owing to the small size of the pocket of center II.…”