Analysis and simulation of complex interactions during dynamic microfiltration ofEscherichia coli suspensions

Meyer, Fernand; Gehmlich, I.; Guthke, Reinhard; Górak, Andrzej; Knorre, W. A.

doi:10.1002/(sici)1097-0290(19980720)59:2<189::aid-bit7>3.0.co;2-d

Cited by 12 publications

(4 citation statements)

References 22 publications

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“…It was found that rod-shaped bacteria formed a cake with 100 times higher resistance than that formed by elliptical bacteria and showed a relationship between the compressibility and the void fraction of the cake. Within a single species of unicellular organisms the main effect has been shown to be the dry cell weight (Meyer et al, 1998). In the case of S. erythraea, as used here in the SCM broth, permeate flux was found to be independent of the fermentation time (Fig.…”

Section: Relationship Between Permeate Flux Broth Viscosity and Hypmentioning

confidence: 99%

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

Davies

Baganz

Ison

et al. 2000

Biotechnol. Bioeng.

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Changes in fermentation media not only affect the performance of the fermentation itself (with regard to the kinetics of biomass and product formation and the yields obtained) but also the initial product‐recovery operations downstream of the fermentor. In this work, microfiltration experiments to remove Saccharopolyspora erythraea biomass from fermentation broth and to recover erythromycin were carried out using two fundamentally different media; a soluble complex medium (SCM) and an oil‐based process medium (OBM). Small‐scale batch fermentations of 14‐L working volume were carried out in triplicate using both media. Broth samples were taken from each fermentation at regular intervals from the end of the exponential‐growth phase onwards. These were then processed using a Minitan II (acrylic), tangential crossflow‐filtration module, fitted with a single 60 cm2 Durapore hydrophilic 0.2 μm membrane, operated in concentration mode. The OBM fermentations produced higher titers of erythromycin but required longer fermentation times due to increased lag phases and slower maximum‐growth rates. The OBM also increased the loading on the membrane; at maximum product titers residual oil concentrations of 3 g · L−1, antifoam concentrations of 2 g · L−1 and flour concentrations estimated at approximately 10 g/L−1 were typical. It was found that both the permeate flux and erythromycin transmission were affected by the choice of medium. The OBM had significantly lower values for both parameters (12.8 Lm−2 h−1 and 89.6% respectively) than the SCM (35.9 Lm−2 h−1 and 96.7% respectively) when the fermentations were harvested at maximum erythromycin titers. Transmission of erythromycin stayed approximately constant as a function of fermentation time for both media, however, for the OBM the permeate flux decreased with time which correlated with an increase in broth viscosity. The relatively poor microfiltration performance of the OBM medium was, however, offset by the higher titers of erythromycin that were achieved during the fermentation. The filtration characteristics of the SCM broth did not show any correlation with either broth viscosity or fermentation time. Image‐analysis data suggested that there was a correlation between hyphal morphology (main hyphal length) and permeate flux (no such correlation was found for the OBM broth). Moreover, it has been shown for the OBM broth that the residual flour had a profound effect on the microfiltration characteristics. The influence of the residual flour was greater than that imposed by the morphology and concentration of the biomass. The understanding of the factors governing the interaction of the fermentation and microfiltration operations obtained in this work provides a first step towards optimization of the overall process sequence. © 2000 John Wiley & Sons, Inc. Biotechnol Bioeng 69: 429–439, 2000.

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Section: Relationship Between Permeate Flux Broth Viscosity and Hypmentioning

confidence: 99%

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

Davies

Baganz

Ison

et al. 2000

Biotechnol. Bioeng.

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“…They solved the problem of flux decline by the design of inlet/outlet, disk structure, and the distance between rotating disk and membrane, mixing rate and TMP. Dynamic Membrane Filter (DMF, Pall Corp., Cortland, NY) appeared as the first commercial system [125,147,182]. This filtration unit houses a 6-in.-diameter stainlesssteel solid disk with a maximum rotating speed of 3450 rpm rotating in the clockwise direction only.…”

Section: Rotating Diskmentioning

confidence: 99%

Extensive review about industrial and laboratory dynamic filtration modules: Scientific production, configurations and performances

Cheng

Xie

Schmitz

et al. 2021

Separation and Purification Technology

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“…This will, of course, be membrane‐specific. Meyer and colleagues (60) observed a flux reduction of 15% when cells were stored for 5 h at 4 °C and 30% reduction with freezing and thawing.…”

Section: Cell Harvestingmentioning

confidence: 99%

Bioprocess Engineering Issues That Would Be Faced in Producing a DNA Vaccine at up to 100 m³ Fermentation Scale for an Influenza Pandemic

Hoare¹,

Levy²,

Bracewell³

et al. 2005

Biotechnology Progress

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The risk of a pandemic with a virulent form of influenza is acknowledged by the World Health Organization (WHO) and other agencies. Current vaccine production facilities would be unable to meet the global requirement for vaccine. As a possible supplement a DNA vaccine may be appropriate, and bioprocess engineering factors bearing on the use of existing biopharmaceutical and antibiotics plants to produce it are described. This approach addresses the uncertainty of timing of a pandemic that precludes purpose-built facilities. The strengths and weaknesses of alternative downstream processing routes are analyzed, and several gaps in public domain information are addressed. The conclusion is that such processing would be challenging but feasible.

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Analysis and simulation of complex interactions during dynamic microfiltration ofEscherichia coli suspensions

Cited by 12 publications

References 22 publications

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

Extensive review about industrial and laboratory dynamic filtration modules: Scientific production, configurations and performances

Bioprocess Engineering Issues That Would Be Faced in Producing a DNA Vaccine at up to 100 m³ Fermentation Scale for an Influenza Pandemic

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Analysis and simulation of complex interactions during dynamic microfiltration ofEscherichia coli suspensions

Cited by 12 publications

References 22 publications

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

Studies on the interaction of fermentation and microfiltration operations: Erythromycin recovery fromSaccharopolyspora erythraea fermentation broths

Extensive review about industrial and laboratory dynamic filtration modules: Scientific production, configurations and performances

Bioprocess Engineering Issues That Would Be Faced in Producing a DNA Vaccine at up to 100 m3 Fermentation Scale for an Influenza Pandemic

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Product

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Bioprocess Engineering Issues That Would Be Faced in Producing a DNA Vaccine at up to 100 m³ Fermentation Scale for an Influenza Pandemic