Analysis and stability of the constituents of St. John's wort oils prepared with different methods

Isacchi, Benedetta; Bergonzi, Maria Camilla; Carnevali, Fiorella; Esch, S; Vincieri, F. F.; Bilia, Anna Rita

doi:10.1016/j.jpba.2007.08.025

Cited by 34 publications

(17 citation statements)

References 14 publications

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“…Numerous papers have been published in qualitative analysis of bioactive constituents in the extract of Hypericum perforatum by HPLC[14–15], HPLC-MS[16–19], HPLC-SPE-NMR[20–21], and capillary electrophoresis[22]. Some reports are related to their extraction and isolation including enrichment of hyperforin by supercritical fluid extraction[23], extraction of hyperoside using CTAB reversed micelles[24], purification of hypericin and pseudohypericin using liquid-liquid extraction and SEC[25], purification of total flavonoid glycosides by macroporous adsorption resin[26], separation of naphthodianthrones by preparative high-performance liquid chromatography[27] etc..…”

Section: Introductionmentioning

confidence: 99%

Isolation and purification of series bioactive components from Hypericum perforatum L. by counter-current chromatography

Cao

Wang

et al. 2011

Journal of Chromatography B

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High-speed counter-current chromatography (HSCCC) combined with pre-separation by ultrasonic solvent extraction was successively used for the separation of series bioactive compounds from the crude extract of Hypericum perforatum L. The petroleum ether extract was separated by the solvent system of n-heptane-methanol-acetonitrile (1.5:0.5:0.5, v/v) and n-heptane-methanol (1.5:1, v/v) in gradient elution, yielding a phloroglucinol compound, hyperforin with HPLC purity over 98%. The ethyl acetate extract was separated by using the solvent system composed of hexane-ethyl acetate-methanol-water (1:1:1:1 and 1:3:1:3, v/v) in gradient through both reverse phase and normal phase elution mode, yielding a naphthodianthrone compound, hypericin with HPLC purity about 95%. The n-butanol extract was separated with the solvent system composed of n-butanol-ethyl acetate–water (1:4:5 and 1.5:3.5:5, v/v) in elution and back-extrusion mode, yielding two of flavones, rutin and hyperoside, with HPLC purity over 95%. HPLC-MS, reference sample and UV spectrum were selectively used in separation to search for target compounds from HPLC-DAD profiles of different sub-extracts. The structures of isolated compounds were further identified by ESI-MS, 1HNMR and 13CNMR.

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Section: Introductionmentioning

confidence: 99%

Isolation and purification of series bioactive components from Hypericum perforatum L. by counter-current chromatography

Cao

Wang

et al. 2011

Journal of Chromatography B

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“…Alcohols are generally used for the extraction of a wide spectrum of polar and non-polar compounds. Methanol is the most commonly used solvent for the extraction and analysis of H. perforatum on a laboratory scale (Isacchi et al., 2007 ; Filippini et al., 2010 ; Tawaha et al., 2010 ). However, since this is classified as toxic, ethanol is preferable in pharmaceutical preparations.…”

Section: Resultsmentioning

confidence: 99%

“…The oil preparations have been extensively studied, but the literature lacks details on the content of active constituents and formulation of the hydroalcoholic preparations (EMEA, 2009 ). Moreover there is a great variability in the concentrations of these active constituents depending on the geographical origin of the plant, the harvest time and the method of extraction (Isacchi et al., 2007 ). Therefore, in order to ensure reproducibility of the in vivo experiments, detailed description of the extraction method should be provided and a chromatographic finger print of the used extracts must be established.…”

Section: Discussionmentioning

confidence: 99%

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An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system

Ali¹,

Motaal

Ahmed

et al. 2018

Drug Delivery

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The active compounds present in Hypericum perforatum L. (Hypericaceae) include hyperforin, hypericins and flavonoids, which are assumed to be responsible for the activity of the extract in the treatment of wounds and scars. The present study aimed to incorporate H. perforatum extract standardized to a known content of phloroglucinols, naphthodianthrones and polyphenolic compounds into an effective transdermal drug delivery system capable of entrapping both lipophilic and hydrophilic constituents in the form of niosomal gels for wound treatment. An 80% ethanol extract (HE) was prepared on a pilot scale using DIG-MAZ. An HPLC-DAD holistic profile was established for HE and was standardized to contain 3.4 ± 4 rutin, 1.1 ± 3 chlorogenic acid, 0.5 ± 2 quercitrin, 2.8 ± 2 hyperforin, and 0.51 ± 3% w/w total hypericins. Niosomes were prepared using the modified reverse phase evaporation technique (REV). The wound healing effect of the gel was tested on 16 adult mongrel dogs. A significant decrease in the inflammatory cell count (18.4 ± 5.3) was recorded in the niosomal gel 1.5% NaCMC-treated group at the 7th day post wounding. It induced a marked regression in the inflammatory phase and enhanced the early beginning of the proliferative phase of wound healing. After 21 days, it showed complete re-epithelization, formation of new matrix fibers and significant reduction in the wound size, compared to the control and the Panthenol® 2% cream treated groups. This is the first study of H. perforatum in a niosomal topical drug delivery system.

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“…Data concerning Oleum Hyperici chemical composition and pharmacological activity are limited. Studies proved the presence of hyperforins and its analogues as well as of flavonoids, quercetin and I3,II8-biapigenin, but the absence of the naphthodianthrones (hypericin) [ 8 , 9 ], although Isacchi et al claimed only the presence of phloroglucinol derivates and I3,II8-biapigenin [ 10 ]. Investigation of St. John’s Wort oil preparations showed that a mixture of St. John’s Wort and Calendula arvensis oil extracts used externally had positive effects on epithelial reconstruction of surgical wounds [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

Hypericum Perforatum L. Hypericaceae/Guttiferae Sunflower, Olive and Palm Oil Еxtracts Attenuate Cold Restraint Stress – Induced Gastric Lesions

et al. 2010

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Three Hypericum perforatum L., Hypericaceae (St. John’s Wort) oil extracts (HPE) were prepared according to the prescriptions from traditional medicine – fresh flowering tops were macerated in three different vegetable oils: sunflower (E1), olive (E2) and palm oil (E3) for 40 days, exposed to the sunlight. The aim of the study was to investigate the gastroprotective activity of the obtained extracts in respect to their quercetin content. HPLC analysis confirmed the presence of quercetin in all of the investigated HPEs, but in different amounts: 15.1, 5.8 and 21.7 μg/mL in E1, E2, and E3, respectively. Gastroprotective activity was evaluated using cold-restraint stress (CRS) induced rat gastric mucosa lesions test. All of the HPEs showed gastroprotective activity, which was close to that achieved by the one of the most studied anti-ulcer flavonoids, quercetin [percentages of inhibition of ulcer index (UI) were 35, 62 and 40 % in E1, E2 and E3, respectively]. Contrary to the lowest quercetin content, HPE prepared with olive oil (E2) offered the highest protection against gastric damaging action of CRS. It may be assumed that this is due to other constituents of E2, which probably play an additional role in complex gastroprotective activity.

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Analysis and stability of the constituents of St. John's wort oils prepared with different methods

Cited by 34 publications

References 14 publications

Isolation and purification of series bioactive components from Hypericum perforatum L. by counter-current chromatography

Isolation and purification of series bioactive components from Hypericum perforatum L. by counter-current chromatography

An in vivo study of Hypericum perforatum in a niosomal topical drug delivery system

Hypericum Perforatum L. Hypericaceae/Guttiferae Sunflower, Olive and Palm Oil Еxtracts Attenuate Cold Restraint Stress – Induced Gastric Lesions

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