To study the influence of tip clearance on the working characteristics of high-altitude fans, this paper takes the MIX-140 high-altitude fan as the research object. Five different tip clearance (TC) models are selected. The CFD method is used to analyze the changes in the working characteristics of the fan under different flow rates and TCs in ground and high-altitude environments. The reliability of the numerical method is verified through a fan test bench. The results show that the tip leakage flow caused by the TC will continuously deteriorate the working characteristics of the fan. Under the rated flow rate in the ground environment, for every doubling of the blade’s TC, the static pressure difference will decrease by 5%, the efficiency will decrease by 2%, and the power will decrease by 3%. In a high-altitude environment, the flow rate corresponding to the maximum shaft power point of the fan will continuously increase with an increase in the tip clearance, which will bring about additional energy consumption. For high-altitude fans, the deformation caused by the high-speed rotation of the impeller needs to be taken into account. Undoubtedly, it is advantageous to choose the smallest possible tip clearance value. The results of the analysis and test methods in this paper will provide a basis for designing the tip clearance of high-altitude fans.