Analysis at the genomospecies level of microbial populations changes in activated sludge: the case of Aeromonas

Nsabimana, E

doi:10.1016/s0043-1354(99)00298-5

Cited by 12 publications

(3 citation statements)

References 39 publications

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“…The probes were used to quantify the abundance of Desulfotomaculum in thermophilic anaerobic digesters, soil, human feces, and pig colon samples. Nsabimana et al (2000) used ribotyping to produce rRNA restriction patterns of Aeromonas in activated sludge. They found that Aeromonas species were more diverse when a municipal activated sludge plant was receiving influent with high BOD, COD, ammonia and phosphate levels.…”

Section: Filamentous Microorganisms Microorganisms Demonstrating Mormentioning

confidence: 99%

Molecular Methods in Biological Systems

Oerther¹,

Reyes²

2001

Water Environment Research

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los Reyes III important phosphorus accumulating organism (PAO) involved in EBPR. FISH with domain-, subdivision-, and genus-level probes to show that alpha-and beta-subclass Proteobacteria were dominant in an EBPR system (3-stage Phoredox process) (Mudaly et al., 2000). You et al. (2000) used restriction fragment length polymorphism (RFLP) and denaturing gradient gel electrophoresis (DGGE) to compare the microbial communities in suspended growth and biofilm nutrient removal processes. The cloning procedure used resulted in more clones from the b-Proteobacteria in the suspended growth process, and they concluded that the differences in microbial community structure were due to the configuration (suspended vs. attached growth) rather than to COD/P ratios. DGGE and biomarkers (respiratory quinines and cellular fatty acids) were used to characterize the microbial community changes in lab-scale EBPR systems in response to altering sludge content . The authors found that EBPR systems had similar microbial community structures, and these were different from systems with no EBPR activity. They concluded that the sludge P content had an effect on the composition and abundance of predominant microbial populations, though specific phylotypes could not be associated with EBPR.

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Section: Filamentous Microorganisms Microorganisms Demonstrating Mormentioning

confidence: 99%

Molecular Methods in Biological Systems

Oerther¹,

Reyes²

2001

Water Environment Research

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“…Members of the genus Aeromonas prevail in various aquatic habitats (Huang et al, 2019; Huys, 2014; Janda & Abbott, 2010; Nsabimana et al, 2000; Sanglas et al, 2017; VandeWalle et al, 2012) and play important roles in biological wastewater treatment and environmental remediation due to their metabolic diversities (Cao et al, 2012; Chung & Okahe, 2009; Park et al, 2008). They can respire with various organic and inorganic electron acceptors, including chlorinated organic compounds, azo dyes, nitrate, vanadium (V(V)), cobalt (Co(III)), selenium (Se(VI)), chromium (Cr(VI)), and iron oxides (Cao et al, 2012; Knight & Blakemore, 1998; T. X. Liu et al, 2014; X. S. Wu et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Extracellular electron transfer via multiple electron shuttles in waterborneAeromonas hydrophilafor bioreduction of pollutants

Min

Liu

et al. 2021

Biotech & Bioengineering

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Members of the genus Aeromonas prevail in aquatic habitats and have a great potential in biological wastewater treatment because of their unique extracellular electron transfer (EET) capabilities. However, the mediated EET mechanisms of Aeromonas have not been fully understood yet, hindering their applications in biological wastewater treatment processes. In this study, the electron shuttles in Aeromonas hydrophila, a model and widespread strain in aquatic environments and wastewater treatment plants, were explored. A. hydrophila was found to produce both flavins and 2-amino-3-carboxy-1,4-naphthoquinone (ACNQ) as electron shuttles and utilize them to accelerate its EET for the bioreduction of various pollutants.The Mtr-like respiratory pathway was essential for the reduction of flavins, but not involved in the ACNQ reduction. The electron shuttle activity of ACNQ for pollutant bioreduction involved the redox reactions that occurred inside the cell. These findings deepen our understanding about the underlying EET mechanisms in dissimilatory metal reducing bacteria and provide new insights into the roles of the genus Aeromonas in biological wastewater treatment.

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“…Members of the genus Aeromonas are widely present in aquatic environments, − and many of them are critically involved in transformation of environmental pollutants including metals, dyes, organic arsenic compounds, and even persistent organic pollutants., − Many Aeromonas species are capable of extracellular respiration and utilizing diverse carbon sources (at least 11 types). − Such a metabolism diversity renders them with unique advantages over most extracellular respiration bacteria including Geobacter sulfurreducens and Shewanella oneidensis in environmental adaptability and lays a foundation for their important roles in wastewater treatment, environmental remediation, and geochemical processes. − However, biomolecular mechanisms underpinning their diverse metabolic pathways for pollutant transformation remain poorly understood to date.…”

Section: Introductionmentioning

confidence: 99%

CRISPRi System as an Efficient, Simple Platform for Rapid Identification of Genes Involved in Pollutant Transformation by Aeromonas hydrophila

Cheng

Min

et al. 2020

Environ. Sci. Technol.

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Aeromonas species are indigenous in diverse aquatic environments and play important roles in environmental remediation. However, the pollutant transformation mechanisms of these bacteria remain elusive, and their potential in pollution control is largely unexploited so far. In this work, we report an efficient and simple genome regulation tool to edit Aeromonas hydrophila and identify its biomolecular pathways for pollutant transformation. The genome regulation system, which is based on the type II clustered regularly interspaced short palindromic repeat interference (CRISPRi) system from Streptococcus pyogenes, can serve as a reversible and multiplexible platform for gene knockdown in A. hydrophila. A single-plasmid CRISPRi system harboring both dCas9 and the sgRNA was constructed in A. hydrophila and used to silence diverse genes with varied sizes and expression levels. With this system, up to 467-fold repression of gfp expression was achieved, and the function of the essential gene-ftsZ was identified quickly and accurately. Furthermore, simultaneous transcriptional repression of multiple targeted genes was realized. We discovered that the ars operon played an essential role in arsenic detoxification, and the extracellular electron transfer (EET) pathway was involved in methyl orange reduction, but not in vanadium reduction by A. hydrophila. Our method allows better insights and effective genetic manipulation of the pollutant transformation processes in Aeromonas, which might facilitate more efficient utilization of the Aeromonas species and other microbial species for environmental remediation applications.

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Analysis at the genomospecies level of microbial populations changes in activated sludge: the case of Aeromonas

Cited by 12 publications

References 39 publications

Molecular Methods in Biological Systems

Molecular Methods in Biological Systems

Extracellular electron transfer via multiple electron shuttles in waterborneAeromonas hydrophilafor bioreduction of pollutants

CRISPRi System as an Efficient, Simple Platform for Rapid Identification of Genes Involved in Pollutant Transformation by Aeromonas hydrophila

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