Analysis by Dansylation of Amino Acids Dissolved in Marine and Freshwaters1

Litchfield, Carol D.; Prescott, J. M.

doi:10.4319/lo.1970.15.2.0250

Cited by 18 publications

(2 citation statements)

References 10 publications

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“…The levels of DFAA in sea water have been determined in a number of studies (e.g. Siege1 & Degens, 1966; Bohling, 1970;Litchfield & Prescott, 1970;Riley & Segar, 1970;Pocklington, 1972;Schell, 1974). A wide range of concentrations have been reported, depending on factors such as analytical methods, location, season and depth.…”

Section: )mentioning

confidence: 99%

The detection of dissolved amino acids by the Atlantic cod, Gadus morhua L.

Johnstone

1980

Journal of Fish Biology

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A classical conditioning technique was employed with cod, Gadus morhua L., to determine thresholds for the detection of the L-forms of some a amino acids which are thought to be attractants or feeding stimulants for fish. The amino acids investigated, in order of effectiveness, were tyrosine, cysteine, phenylalanine, glycine and methionine with mean threshold response levels ranging from 2.5 x lo-% to 7.4 x lo-". Histidine and lysine resulted in similar thresholds with a mean value of approximately 3 x lo-% while taurine and leucine were least effective with mean threshold levels of 2.1 x 1 0 -6~ and 2.1 x IO+M respectively. Comparison is made with electrophysiological and behavioural response data from other species. The effect of raising the background level of glycine on the threshold to glycine for cod is described. The results are discussed with reference to data on levels of dissolved free amino acids in shallow sea waters which may have a bearing on determining chemosensory threshold levels. To detect a specific amino acid against a background level of the same substance the difference in level for detection is proportionally greater for higher background concentrations.

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Section: )mentioning

confidence: 99%

The detection of dissolved amino acids by the Atlantic cod, Gadus morhua L.

Johnstone

1980

Journal of Fish Biology

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“…Attempts to characterize DON in fresh and brackish water have employed evaporation followed by microKjeldahl digestion (Domogalla, Juday and Peterson, 1925;Domogalla and Fred, 1926;Birge and Juday, 1934;Rainwater and Thatcher, 1960;Robertson and Powers, 1968); phosphotungstic acid precipitation (Domogalla et al, 1925); --sodium decomposition (Shapiro, 1957); evaporation, gelfiltration (Sephadex), ion exchange or dialysis followed by colormetric or chromatographic amino acid analysis (Peterson, Fred and Domogalla, 1925;Schurmann, 1964;Brehm, 1967;Sieburth and Jensen, 1968;Litchfield and Prescott, 1970;Gocke, 1970;Wetzel, unpublished); kinetic bioassay of added amino acids (Hobbie, Crawford, and Webb, 1968;Hobbie and Crawford, 1969); and N labeling 15 (Jones andStewart, 1969a, 1969b).…”

Section: Previous Don Analytical H Techniquesmentioning

confidence: 99%

Interactions of dissolved and particulate nitrogen in lake metabolism

Manny¹

1971

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Weekly measurements of dissolved ammonia, nitrite, and nitrate for 22 months and organic nitrogen for 12 months were made in two hardwater lakes and seasonally in four other lakes representing a chemical and trophic spectrum in glaciated Michigan. Seston at 1 m depth was fractionated weekly for 14 months using 10 um Nitex netting in two lakes representing extremes of hardwater productivity.Net and nanno fractions were analyzed by micro-Kjeldahl digestion for organic nitrogen. Seasonal changes in all nitrogenous parameters were subsequently related to changes in fifteen other chemical and biological parameters assayed simultaneousl y.More than half of total sestonic pigments, algal cell volume, and organic nitrogen were present all year in the nannosestonic fraction in the unproductive and productive lake. These data supported the hypothesis that nannophytoplankton contain more nitrogen per unit cell volume than netphytoplankton. DISTRIBUT ION OF THIS DOCUMEN T IS UNLIMITEDfl DISCLAIMER This report was prepared as an account of work sponsored by an agency of the United States Government. Neither the United States Government nor any agency Thereof, nor any of their employees, makes any warranty, express or implied, or assumes any legal liability or responsibility for the accuracy, completeness, or usefulness of any information, apparatus, product, or process disclosed, or represents that its use would not infringe privately owned rights. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise does not necessarily constitute or imply its endorsement, recommendation, or favoring by the United States Government or any agency thereof. The views and opinions of authors expressed herein do not necessarily state or reflect those of the United States Government or any agency thereof. DISCLAIMERPortions of this document may be illegible in electronic image products. Images are produced from the best available original document.

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Organic Compounds

Analysis of Seawater

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Analysis by Dansylation of Amino Acids Dissolved in Marine and Freshwaters1

Cited by 18 publications

References 10 publications

The detection of dissolved amino acids by the Atlantic cod, Gadus morhua L.

The detection of dissolved amino acids by the Atlantic cod, Gadus morhua L.

Interactions of dissolved and particulate nitrogen in lake metabolism

Organic Compounds

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