Analysis by reflectance spectroscopy of nucleotides resolved on chromatoplates

Lieu, Van T.; Frodyma, Michael M.; Higashi, Lois S.; Kunimoto, Lenora H.

doi:10.1016/0003-2697(67)90236-9

Cited by 12 publications

(6 citation statements)

References 9 publications

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“…The data agreed well with the results reported by Lieu et al 245 The wavelength values for the maxima (Table 10) were found to fluctuate by no more than 2nm, even over extended periods of up to six days and with concentrations varying from 0.5 to 5.0 /ig/spot. The spectra were recorded directly from the plate by focusing the light beam to the center of the spot.…”

Section: Nucko Derivativessupporting

confidence: 91%

“…2 ' 6 Simple heating of the plates will occasionally result in the formation of a colored product 93 and if a fluorescent compound is available, observation under UV light enables location of the spot. 245 For many UV-active compounds detection of the spots as dark areas is possible on a fluorescent background. This fluorescent background can be produced by incorporating a luminous pigment into the adsorbent, 9 4 ' 2 4 6 ' 2 4 7 or by just taking advantage of the natural fluorescence of adsorbents such as silica gel or cellulose.…”

Section: General Experimental Procedures a Detection Of Spotsmentioning

confidence: 99%

“…245 The spots were located under a UV lamp (254 nm) by the fluorescence quenching method, removed from the plate, and packed in a windowless cell (section III A) for measurement. Accuracies were reported to range between 2 and 6%.…”

Section: Nucko Derivativesmentioning

confidence: 99%

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Diffuse Reflectance Spectroscopy

Frei¹,

Zeitlin²

1971

C R C Critical Reviews in Analytical Chemistry

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Section: Nucko Derivativessupporting

confidence: 91%

Section: General Experimental Procedures a Detection Of Spotsmentioning

confidence: 99%

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Diffuse Reflectance Spectroscopy

Frei¹,

Zeitlin²

1971

C R C Critical Reviews in Analytical Chemistry

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“…Diffuse reflectance spectrometry has been used for quantification of spots in paper chromatography (3-5) and in TLC (6)(7)(8)(9)(10)(11)(12), and a review of the TLC techniques has recently appeared (13). For quantitative analysis, TLC zones were usually removed from the plates before making reflectance measurements.…”

Section: Resultsmentioning

confidence: 99%

Thin-layer chromatography of steroidal pharmaceuticals

Hara¹,

Mibe²

1968

Anal. Chem.

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mentioning

confidence: 99%

Determination of reflectance of pesticide spots on thin-layer chromatograms using fiber optics

Beroza¹,

Hill²,

Norris³

1968

Anal. Chem.

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An instrument and method have been devised for scanning thin-layer chromatograms by diffuse reflectance. The heart of the instrument is a fiber-optic scanning head containing randomly mixed glass fibers; half of the fibers conducts light to a small defined area of the thin-layer plate being scanned at a constant rate, and the other half conducts the reflected light to a photosensing cell that has its response recorded. In dual-beam operation a second fiber-optic, which scans the blank area adjacent to the spots, is used to correct for background differences on the plate. In typical analyses, variation attributable to chromatographic technique was between 11 and 16%, while instrumental variation was only 1 to 2%. Data on the performance of the instrument in determining chlorinated and thiophosphate pesticides are presented.

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Analysis by reflectance spectroscopy of nucleotides resolved on chromatoplates

Cited by 12 publications

References 9 publications

Diffuse Reflectance Spectroscopy

Diffuse Reflectance Spectroscopy

Thin-layer chromatography of steroidal pharmaceuticals

Determination of reflectance of pesticide spots on thin-layer chromatograms using fiber optics

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