Analysis Concentration of &amp;lt;i&amp;gt;Toxoplasma gondii&amp;lt;/i&amp;gt; on Anti-Toxoplasma IgG-IgM Antibody Levels, and the Outcomes of Pregnancy in Mice Balb/c

Simanjuntak, Tigor Peniel; Hatta, Mochammad; Sirait, Robert Hotman; Karo, Marni Br; Sirait, Lenny Irmawaty; Aritonang, Tetty Rina; Rauf, Syahrul; Dwiyanti, Ressy

doi:10.4236/ojog.2017.73030

Cited by 5 publications

(4 citation statements)

References 27 publications

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“…Our previous study also found that antitoxoplasma IgM and IgG antibody levels increased significantly 24 hours and 48 hours after injection of 10 T. gondii tachyzoites 5 The expression of FOXP3 gene in this study decreased significantly (p < 0.05) 3 days after injection of 10 T. gondii tachyzoites. The other researchers also reported decreased the FOXP3 gene expression during acute infection [9][10] .…”

Section: Discussionsupporting

confidence: 73%

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Effectiveness Curcuma Longa to Prevent Cells Damage in Early Pregnant Mice with Acute Toxoplasmosis

Simanjuntak,

Hatta,

Rauf

et al. 2019

Obgynia

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Objective: To prove the effectiveness of Curcuma longa to prevent damage cells by analyzing FOXP3 gene expression, TNF-α level, and histopathology of placental tissue. Method: This study was conducted in 20 early pregnant mice, divided in 5 groups (K1-K5). K1-K4 were injected with tachyzoites. K1 and K2 were intervened with Curcuma longa 125 and 500 mg/kg/day. K3 were intervened with spiramycin 60 mg/kg/day, K4 were intervened with 0,2 distilled water, and K5 was not injected and intervened. IgG-IgM levels, FOXP3 mRNA expression, TNF-α level was examined serially, and placental mice were taken for histopathology examination 7 days after intervention.Result: FOXP3 mRNA expression in K1 and K2 increased significantly 7 days after intervention compared to K4 (p < 0.05), however the increased of expression in two groups has no significant difference. TNF-α level in K1 and K2 decreased significantly 7 days after intervention compared to K4 (p < 0.05), however the decrease of level in two groups has no significant difference. Hemorrhagic and necrotic cells were not found in K1 and K2, but were found in K3 (75%) and K4 (100%). Conclusion: Curcuma longa 125 mg is effective to prevent cells damage in early pregnant mice with acute toxoplasmosis. Efektivitas Ekstrak Curcuma Longa Mencegah Kerusakan Sel Pada Mencit Hamil Muda dengan Toksoplasmosis Akut.AbstrakTujuan: Membuktikan efektivitas Curcuma longa mencegah kerusakan sel dengan menganalisis ekspresi gen FOXP3, kadar TNF-α, dan histopatologi jaringan plasenta.Metode: Penelitian dilakukan pada 20 tikus hamil muda, dibagi dalam 5 kelompok (K1-K5). K1-K4 diinjeksi 10 takizoid toksoplasma intra abdominal. Tiga hari pasca injeksi takizoid, K1 dan K2 diintervensi dengan Curcuma longa 125 dan 500 mg / kg / hari. K3 diintervensi dengan spiramisin 60 mg / kg / hari, K4 diintervensi dengan 0,2 ml air suling dan K5 tidak diinjeksi dan tidak diintervensi. Kadar IgG-IgM, ekspresi mRNA FOXP3, kadar TNF-α diperiksa secara serial dan plasenta tikus diambil untuk pemeriksaan histopatologi 7 hari pasca intervensi.Hasil: Ekspresi mRNA FOXP3 pada K1 dan K2 meningkat bermakna (p< 0.05) 7 hari pasca intervensi dibandingkan dengan K4 (p <0,05), tetapi peningkatan ekspresi pada kedua kelompok tidak terdapat perbedaan bermakna. Kadar TNF-α pada K1 dan K2 menurun bermakna 7 hari pasca intervensi dibandingkan dengan K4 (p <0,05), tetapi penurunan kadar pada dua kelompok tidak terdapat perbedaan bermakna. Hemoragik dan nekrotik sel tidak ditemukan pada K1 dan K2, tetapi ditemukan pada K3 (75%) dan K4 (100%).Kesimpulan: Curcuma longa 125 mg efektif untuk mencegah kerusakan sel pada tikus hamil muda dengan toksoplasmosis akut.Key words: Curcuma longa, kerusakan sel, hamil muda, gene FOXP3, TNF-α, toxoplasmosis

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Section: Discussionsupporting

confidence: 73%

“…Toxoplasmosis is an infection caused by the parasite toxoplasma gondii [1][2][3] . Toxoplasma infection will stimulate the cellular and humoral immune response of the body [4][5][6] . The Fork head box P3 (FOXP3) gene as a regulator of the balance of cellular immunity through T-helper cell 7 .…”

Section: Introductionmentioning

confidence: 99%

Effectiveness Curcuma Longa to Prevent Cells Damage in Early Pregnant Mice with Acute Toxoplasmosis

Simanjuntak,

Hatta,

Rauf

et al. 2019

Obgynia

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“…Humoral immunity such as anti-Toxoplasma IgG-IgM antibody level increase in the event of infection of T. gondii . [21] In our study, after injection of T. gondii tachyzoites, the anti-toxoplasma IgG-IgM antibody level increased significantly ( P < 0.05) after 3 days, 6 days, and 10 days Figure 1 and Figure 2.…”

Section: Discussionsupporting

confidence: 50%

Analysis of anti-toxoplasma immunoglobulin G and immunoglobulin M antibody levels after intervention with Curcuma Longa extract on early pregnant mice with acute toxoplasmosis

Simanjuntak

Hatta

Tahir

et al. 2019

J Global Infect Dis

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Background: Curcuma longa has strong anti-inflammatory effect. This study aims to evaluated the level of anti-Toxoplasma immunoglobulin G and immunoglobulin M (IgG-IgM) antibody after intervention with C. longa extract in early pregnant mice with acute toxoplasmosis. Materials and Methods: We evaluated 20 early pregnant mice that were divided into five groups, four mice in each. Group 1-4 received injections of Toxoplasma gondii tachyzoites. Three days later, G1 and G2 were given orally 125 mg/kg/day and 500 mg/kg/day of C. longa extract, respectively. G3 was given 60 mg/kg/day of spiramycin (positive control), and G4 was given 0.2 ml of distilled water (negative control). G5 underwent no intervention at all. Blood samples were obtained serially (before and 3 days after injection of tachyzoites, 3 days and 7 days after intervention) to assess anti-Toxoplasma IgG-IgM antibody levels by enzyme-linked immunosorbent assay methods. Results: Anti-Toxoplasma IgG-IgM antibody levels increased significantly 3 days after injection of tachyzoites ( P < 0.05), but decreased significantly ( P < 0.05) 3 days, and 7 days after administration of C. longa extract dose 125 mg, 500 mg, and spiramycin 60 mg, and there was no significant difference between these three groups. Anti-Toxoplasma IgG-IgM antibody levels increased significantly ( P < 0.05) 3 days, 6 days, and 10 days after injections of tachyzoites on G4. The IgG-IgM antibody levels fluctuated on G5 and considered as insignificant ( P > 0.05). Conclusion: The administration of C. longa extract at a dose of 125 mg/kg/day for 7 days effectively decreased anti-Toxoplasma IgG-IgM antibody level in early pregnant mice with acute toxoplasmosis.

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“…the most polyxenous protozoan [1,2] . Approximately one third of the human population are infected with Toxoplasma gondii [3] , thus this parasite ranks third among the most widespread parasitic diseases throughout the world [4]. Toxoplasma gondii has emerged as a major opportunistic pathogen of immunocompromised individuals [5] .…”

Section: Introductionmentioning

confidence: 99%

Sex-Related Effects on Latent Toxoplasmosis Course Induced in Mice

Hegazy

Azab

El-Tantawy

et al. 2015

Mansoura Medical Journal

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Background: Sex-associated hormones influence the severity of Toxoplasma gondii infection especially during pregnancy and if immmunosuppression takes place. Aim of the study: So, the aim of our work is to study sex-related effects on the course of chronic toxoplasmosis in mice. Methods: Twenty male and 20 female mice were artificially infected with cystogenic type II ME49 Toxoplasma strain. The morbidity was assessed in both sexes in acute stage by observing clinical signs of toxoplasmosis besides examination of peritoneal aspirate for tachyzoites. In chronic stage, morbidity was assessed by estimation of brain cyst burden, histopathological examination and monitoring serum anti-Toxoplasma IL-12 using ELISA. Results: There was no significant difference between males and females regarding clinical signs in acute stage. In chronic stage, females have more brain cyst count and more pathological lesions than males. Moreover, the results obtained by measurement of anti-Toxoplasma IL-12 showed high levels in male than female group. Conclusion: Our results demonstrated that male mice are less susceptible to infection while, female ones are more susceptible and showing exaggerated inflammatory

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Analysis Concentration of <i>Toxoplasma gondii</i> on Anti-Toxoplasma IgG-IgM Antibody Levels, and the Outcomes of Pregnancy in Mice Balb/c

Cited by 5 publications

References 27 publications

Effectiveness Curcuma Longa to Prevent Cells Damage in Early Pregnant Mice with Acute Toxoplasmosis

Effectiveness Curcuma Longa to Prevent Cells Damage in Early Pregnant Mice with Acute Toxoplasmosis

Analysis of anti-toxoplasma immunoglobulin G and immunoglobulin M antibody levels after intervention with Curcuma Longa extract on early pregnant mice with acute toxoplasmosis

Sex-Related Effects on Latent Toxoplasmosis Course Induced in Mice

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