Analysis Methods of Phenolic Acids

Xu, Zhimin

doi:10.1002/9781118229378.ch3

Cited by 12 publications

(16 citation statements)

References 128 publications

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“…Our results were in agreement with previous studies that purified the phenolic compounds by adsorption chromatography using a silica gel column and HPLC techniques [25,26,44]. There are many techniques for phenolics purification depending on the type of these compounds and their chemical structures.…”

Section: Resultssupporting

confidence: 93%

“…The resulting concentrated crude extract from P. dactylifera pits was partially purified using adsorption chromatography by silica gel (mesh 60-120) column. The slurry of silica gel was prepared by soaking with hexane [24][25][26], subsequently with absolute ethanol and then poured into the column (25 cm×2.5 cm) and washed with ethanol for 1 hr to obtain better packing. Concentrated plant extract (5 ml) was loaded into silica gel column and eluted successively with different solvents using batch ways (500 ml for each) 95% ethanol, 70% acetone, 30% acetone, and deionized water.…”

Section: Phenolics Of P Dactylifera Purificationmentioning

confidence: 99%

“…The solvent was run at 4 ml/min and 5 ml fraction volume was collected. Frequently, each fraction was checked by absorbance at 275 nm for phenolic acids and isoflavonoids and 340 nm for flavonoids and coumestans using a spectrophotometer (PD-303 ultraviolet [UV], APEL Co., Ltd., Japan) [24][25][26]. then estimating phenolic and flavonoid compounds content.…”

Section: Phenolics Of P Dactylifera Purificationmentioning

confidence: 99%

“…The dried sample of 70% acetone fraction was further purified by preparative high-performance liquid chromatography (PHPLC). They were dissolved in methanol, filtered through a 0.45 µm nylon filter, and injected (2 ml) into the PHPLC system (JASCO-FC2088-30)/Japan, on a C 18 (300 mm×50 mm, 10 µm) column using mobile phase acetonitrile at a flow rate of 1 ml/min and with a detection wavelength of 254 nm [24][25][26].…”

Section: Phenolics Of P Dactylifera Purificationmentioning

confidence: 99%

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Preventive Effects of Phoenix Dactylifera Polyphenols Against 7,12-Dimethylbenz(a)anthracene-Induced Mammary Cancer

Omran

Al-taee

Hashim

et al. 2017

Asian J Pharm Clin Res

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Objectives: Polyphenols are vital micronutrients, in our diet, which have a role in the prevention of progressive cancer and cardiovascular diseases developing. The main objective of this research was to evaluate the hemopreventive effects of some polyphenols of Phoenix dactylifera pits on 7,12-dimethylbenz(a)anthracene (DMBA)-induced mammary cancer of female albino rats.Methods: The phenolics of P. dactylifera pits (Zahidi cultivar) were prepared by successive steps; extraction by ethanol:methanol:HCl:H2O, adsorption chromatography using a silica gel column and preparative high performers chromatography. The cytotoxic activity of the phenolics was detected against human breast cancer cell line (MCF-7). The acetone phenolic fraction, 50 female albino rats, and DMBA carcinogen were used to study the preventive effects.Results: The acetone phenolic fraction contained considerable amounts of the total phenolics and having antioxidant activity, which inhibit cancer cell line MCF-7 growth, and the inhibitory concentration 50% of cells (IC50) at 24 hrs was 202 μg/ml for MCF-7 and 480 μg/ml for WRL-68. The purified phenolic compounds may be related to genistein and quercetin derivatives that inhibited MCF-7 cell line growth and IC50 were 1030 and 203.9 μg/ml, respectively, compared with negligible effects on normal cell line. The acetone phenolic fraction prevented mammary cancer formation in the DMBA-induced rat model.Conclusions: The phenolics of date palm had chemopreventive effects against DMBA-induced mammary cancer, and they required further research to clarify the possible mechanisms that might have contributed to the preventive effects against mammary cancer.

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Section: Resultssupporting

confidence: 93%

Section: Phenolics Of P Dactylifera Purificationmentioning

confidence: 99%

Section: Phenolics Of P Dactylifera Purificationmentioning

confidence: 99%

Section: Phenolics Of P Dactylifera Purificationmentioning

confidence: 99%

See 2 more Smart Citations

Preventive Effects of Phoenix Dactylifera Polyphenols Against 7,12-Dimethylbenz(a)anthracene-Induced Mammary Cancer

Omran

Al-taee

Hashim

et al. 2017

Asian J Pharm Clin Res

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“…The content of total flavonoids was determined using the spectrophotometer method described in the study of Jayaprakasha et al . () The contents of catechin and caffeine in the fortified products were quantified using the methods of Xu (2012b).…”

Section: Methodsmentioning

confidence: 99%

Fortification of the Health Benefit of Buckwheat (F agopyrum tataricum ) Tea

Qin

Zeng

2013

Journal of Food Processing and Preservation

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Three extracts from Zijuan (Camellia sinensis), Kudingcha (Ilex kudingcha) and Mingri (Angelica keiskei) leaves were used to fortify the health promoting function of traditional buckwheat tea. Three fortified teas with similar sensory property to the original tea and higher level of total flavonoids and catechin were chosen to evaluate their capability in lowering hyperglycemic and hyperlipidemic stress using mouse models. The blood glucose level in the hyperglycemic mice was significantly reduced by 10.84 to 34.12% after oral gavage of the three fortified teas (0.4 mL of 20% in solution) each day for 7 days, while it only lowered 4.56% in the original buckwheat tea treatment (P < 0.05). Also, the three fortified teas had effective response to lower the boosted blood glucose level in fasted mice. In the hyperlipidemic mouse model, the mice fed with the fortified teas for 30 days had lower serum total cholesterol and triglyceride levels and higher high‐density lipoprotein‐cholesterol level. Practical Applications This study demonstrated a potential method to enhance the health benefit of buckwheat tea without loss of its sensory properties. The information would be valuable in developing various health‐promoting food products from buckwheat and other grains.

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Identification and antioxidant activity of sinapic acid derivatives in Brassica napus L. seed meal extracts

Siger

Czubiński

Dwiecki

et al. 2013

Euro J Lipid Sci & Tech

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Sinapic acid and its derivatives are main phenolic compounds present in rapeseed. Identification and quantitative determination of those compounds were carried out in crude extracts as well as extracts after basic and acidic hydrolysis. The total phenolic content in the crude extracts, expressed as sinapic acid equivalent, ranged from 1577 to 1705 mg/100 g for cv. Visby and Bellevue, respectively. An increase in total phenolics contents was observed in extracts after hydrolysis in comparison to crude extracts. The predominant phenolic acids in rapeseed samples were trans‐sinapic and cis‐sinapic acids. The highest concentration of sinapic acid methyl ester (2533–2702 mg/100 g) was detected in the extract after basic hydrolysis. In turn, two sinapic acid derivatives, i.e., kaempferol 3‐dihexoside‐7‐sinapoyl‐hexoside and 3‐hexoside‐7‐sinapoyl‐hexoside, were detected in the hydrolysed extracts. Total peroxyl radical‐trapping potential (TRAP) of the investigated crude extracts amounted to 20.21 and 27.05 μM Trolox Eq/g in the case of cv. Visby and Bellevue, respectively. The increase in antioxidant activity of the analyzed extracts after basic and acidic hydrolysis was probably a result of the release of free phenolic compounds from their conjugates (esters, glycosides). Practical applications: The content and qualitative composition of the phenolic compounds in rapeseed have much influence on the stability, sensory, and nutritional characteristics of the products. During oil production process phenolic compounds may be transferred into final products and inhibit lipid oxidation. Rapeseed oil, among all commercially available oils, has the highest content of phenolic compounds, especially sinapic acid and their derivatives. That is why the antioxidant activity of phenolic compounds was investigated in the presented study. Determination of polar phenolics before and after hydrolysis indicates which compounds are responsible for antioxidant effect in oils and which are available for the human organism after digestion in the digestive tract.

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Analysis Methods of Phenolic Acids

Cited by 12 publications

References 128 publications

Preventive Effects of Phoenix Dactylifera Polyphenols Against 7,12-Dimethylbenz(a)anthracene-Induced Mammary Cancer

Preventive Effects of Phoenix Dactylifera Polyphenols Against 7,12-Dimethylbenz(a)anthracene-Induced Mammary Cancer

Fortification of the Health Benefit of Buckwheat (F agopyrum tataricum ) Tea

Identification and antioxidant activity of sinapic acid derivatives in Brassica napus L. seed meal extracts

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