Analysis of 2-Alkylcyclobutanones in Cashew Nut, Nutmeg, Apricot Kernel, and Pine Nut Samples: Re-evaluating the Uniqueness of 2-Alkylcyclobutanones for Irradiated Food Identification

Leung, Elvis Ming Kit; Tang, Phyllis Nya Yang; Ye, Yuran; Chan, Wan

doi:10.1021/jf403500j

Cited by 16 publications

(3 citation statements)

References 26 publications

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“…Analytical results of showed that 2-ACBs were only detected in irradiated samples. The authors concluded that 2-ACBs either do not exist in non-irradiated nuts or that they may be present at very low concentrations for reliable detection (Leung et al 2013). …”

Section: Chemistry Of Major Secondary Metabolites Of Nutmegmentioning

confidence: 99%

Chemical diversity and pharmacological significance of the secondary metabolites of nutmeg (Myristica fragrans Houtt.)

Abourashed

El‐Alfy

2016

Phytochem Rev

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Nutmeg is a valued kitchen spice that has been used for centuries all over the world. In addition to its use in flavoring foods and beverages, nutmeg has been used in traditional remedies for stomach and kidney disorders. The antioxidant, antimicrobial and central nervous system effects of nutmeg have also been reported in literature. Nutmeg is a rich source of fixed and essential oil, triterpenes, and various types of phenolic compounds. Many of the secondary metabolites of nutmeg exhibit biological activities that may support its use in traditional medicine. This article provides an overview of the chemistry of secondary metabolites isolated from nutmeg kernel and mace including common methods for analysis of extracts and pure compounds as well as recent approaches towards total synthesis of some of the major constituents. A summary of the most significant pharmacological investigations of potential drug leads isolated from nutmeg and reported in the last decade is also included.

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Section: Chemistry Of Major Secondary Metabolites Of Nutmegmentioning

confidence: 99%

Chemical diversity and pharmacological significance of the secondary metabolites of nutmeg (Myristica fragrans Houtt.)

Abourashed

El‐Alfy

2016

Phytochem Rev

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“…The application of liquid chromatography-tandem mass spectrometry (LC-MS/MS) to determine 2-ACBs has been recently reported [16,[22][23][24][25]. Although the instrument is more Electronic supplementary material The online version of this article (doi:10.1007/s00216-016-9455-6) contains supplementary material, which is available to authorized users.…”

Section: Introductionmentioning

confidence: 99%

Determination of 2-alkylcyclobutanones by combining precolumn derivatization with 1-naphthalenyl hydrazine and ultra-performance liquid chromatography with fluorescence detection

et al. 2016

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2-Alkylcyclobutanones (2-ACBs) are uniquely formed when triglycerides-containing food products are exposed to ionizing radiation. Thus, 2-ACBs have been used as marker molecules to identify irradiated food. Most methods to determine 2-ACBs involve mass spectrometric detection after chromatographic separation. The spectrofluorometer is rarely used to determine 2-ACBs because these molecules do not fluoresce. In this study, we developed an ultra-performance liquid chromatography (UPLC) method to determine 2-ACBs. 2-ACBs were converted into fluorophores after reacting with 1-naphthalenyl hydrazine to facilitate their sensitive and selective detection using a fluorescence detector (FLD). Analysis of 2-ACBs using our developed UPLC-FLD method allows sensitive determination of 2-ACBs at a detection limit of 2 ng 2-ACBs per g of fat (30 pg/injection), which is significantly lower than that of existing analytical methods. After validation for trueness and precision, the method was applied to γ-irradiated chicken samples to determine their 2-ACB content. Comparative studies employing liquid chromatography-tandem mass spectrometric method revealed no systematic difference between the two methods, thereby demonstrating that the proposed UPLC-FLD method can be suitably used to determine 2-ACBs in irradiated foodstuffs. Graphical Abstract Determination of radiation-induced food-borne 2-dodecylcyclobutanone and 2-tetradecylcyclobutanone by combining 1-naphthalenyl hydrazine derivatization and ultra-performance liquid chromatography with fluorescence detection.

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“…A variety of analytical methods including HPLC with photodiode array detection (PDA), high‐performance TLC, GC–MS, ultra fast LC, HPLC with postcolumn derivatization (PCD) and fluorescence detection (FLD), NMR spectroscopy, and LC–MS have been reported for identification and determination of major bioactive constituents and mycotoxins in fruit extract of M. fragrans . The reported analytical methods (HPLC–PDA, high‐performance TLC, GC–MS, NMR spectroscopy, and HPLC–PCD–FLD) contributed significantly to the current state of knowledge of constituents in the fruits but these have drawbacks of long analysis time, high solvent consumption, low sensitivity, and the need for derivatization.…”

Section: Introductionmentioning

confidence: 99%

Ultra high performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of multiple bioactive constituents in fruit extracts of Myristica fragrans and its marketed polyherbal formulations using a polarity switching technique

Pandey

Rameshkumar

Kumar

2015

J of Separation Science

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Fruits of Myristica fragrans Houtt. are the source of two valuable spices: nutmeg and mace, traditionally used for its flavoring and medicinal properties and found as an ingredient in many marketed polyherbal formulations and food products. In this study, a sensitive and efficient ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry method was developed and validated for the rapid determination of 16 bioactive constituents in different parts of the fruit of M. fragrans and its marketed polyherbal formulations using a polarity switching technique. Chromatographic separation was achieved on an Aquity UPLC BEH C18 column in 9.4 min. Quantitative analysis was performed using multiple reaction monitoring mode with continuous polarity switching in a single analysis. The developed method was found to be accurate with overall recovery in the range from 95.95 to 102.07% (RSD ≤ 1.91%), precise (RSD ≤ 1.98%), and linear (r(2) ≥ 0.9992) over the concentration range of 0.1-200 ng/mL. Quantitative analysis indicated that the total content of the 16 bioactive constituents was highest in the mace of M. fragrans. Thus, this rapid and sensitive method could be utilized as a promising reference method for the quality control of M. fragrans and its marketed herbal formulations/food products.

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Analysis of 2-Alkylcyclobutanones in Cashew Nut, Nutmeg, Apricot Kernel, and Pine Nut Samples: Re-evaluating the Uniqueness of 2-Alkylcyclobutanones for Irradiated Food Identification

Cited by 16 publications

References 26 publications

Chemical diversity and pharmacological significance of the secondary metabolites of nutmeg (Myristica fragrans Houtt.)

Chemical diversity and pharmacological significance of the secondary metabolites of nutmeg (Myristica fragrans Houtt.)

Determination of 2-alkylcyclobutanones by combining precolumn derivatization with 1-naphthalenyl hydrazine and ultra-performance liquid chromatography with fluorescence detection

Ultra high performance liquid chromatography tandem mass spectrometry method for the simultaneous determination of multiple bioactive constituents in fruit extracts of Myristica fragrans and its marketed polyherbal formulations using a polarity switching technique

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