Analysis of 3-(N-Oxalyl)-L-2,3-diaminopropanoic Acid and Its .alpha.-Isomer in Grass Pea (Lathyrus sativus) by Capillary Zone Electrophoresis

Arentoft, Anne Marie; Greirson, Brendon N.

doi:10.1021/jf00052a018

Cited by 32 publications

(17 citation statements)

References 7 publications

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“…The polyphenolic compound (tannins) level was estimated using the method described by Waniska et al (1992). The neurotoxin concentration (b-ODAP) was measured by the technique of capillary electrophoresis on the apparatus Spectra Phoresis 1000 following the protocol of Arentoft and Greirson (1995) with glass capillary 70 cm 9 35 lm, 200 mM phosphate buffer of pH 8.6, and voltage 25 kV, temperature 26.5-35°C and the wave length 195 nm.…”

Section: Chemical Analysesmentioning

confidence: 99%

Variability of phenotypic and morphological characteristics of some Lathyrus sativus L. and Lathyrus cicera L. accessions and nutritional traits of their seeds

Grela

Rybiński

Matras

et al. 2012

Genet Resour Crop Evol

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The object of the study comprised 54 grass pea (Lathyrus sativus) accessions originating from Czech Republic, Hungary, Slovak Republic, Poland, Ukraine and Russia together with 18 red pea (Lathyrus cicera) accessions from Greece, Spain and Italy. The plants were grown under the same conditions in Poland. Some phenological and morphological traits of plants as well as some seed morphological properties and nutritive value were investigated. Lathyrus sativus seeds contained more protein (average 300 g vs. 255 g kg -1 dry matter (DM)), whereas the differences in the average contents of the other basic nutrients between both these species as well as between particular accessions were not substantial. Except for a low level of methionine, the protein of both species showed fairly high concentration of other exogenic amino acids (EAAI around 63-64%). Fat of both L. sativus and L. cicera seeds was found to contain a high level of polyunsaturated fatty acids (66.9 and 58.6%, respectively), mainly linoleic (57. DM, respectively. Average content of this ANF in L. sativus significantly correlated with the colour of both, flowers and seeds; with generally, a darker colour associated with higher levels of tannins.

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Section: Chemical Analysesmentioning

confidence: 99%

Variability of phenotypic and morphological characteristics of some Lathyrus sativus L. and Lathyrus cicera L. accessions and nutritional traits of their seeds

Grela

Rybiński

Matras

et al. 2012

Genet Resour Crop Evol

View full text Add to dashboard Cite

show abstract

“…The polyphenolic compound (tannins) level was estimated according to the method described by Waniska et al (1992). The content of neurotoxin (b-ODAP) was measured by the technique of capillary electrophoresis on the apparatus Spectra Phoresis 1000 following the protocol of Arentoft and Greirson (1995) with glass capillary 70 cm 9 35 um, 200 mM phosphate buffer of pH 8.6, and voltage 25 kV, temperature 26.5-35°C and the wave length 195 nm.…”

Section: Chemical Analysismentioning

confidence: 99%

Morphological characteristics of some accessions of grass pea (Lathyrus sativus L.) grown in Europe and nutritional traits of their seeds

Grela

Rybiński

Klebaniuk

et al. 2010

Genet Resour Crop Evol

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Thirty-one European accessions of grass pea (Lathyrus sativus L.) originating from Italy, Spain, Northern France, Germany and Poland grown in the same soil-climatic conditions in Poland were investigated. The morphological traits as well as the seed morphology and nutrient contents were studied. Substantial differentiation in some morphological characteristics was noticed. The plants could be assigned to two groups: one group comprising the lines from the Mediterranean basin (Italy and Spain) and the other group containing the accessions from West-central Europe (Northern France, Germany and Poland). The grass pea plants produced from the seeds obtained from West-central Europe appeared to be about 10 cm taller than the Mediterranean accessions and had predominantly white flowers. Their seeds were more than two times smaller than seeds of the accessions of Italian or Spanish origin. There were not noted any considerable differences in nutrient contents or antinutritional factors in seeds between these two groups of Lathyrus accessions. A negative correlation between b-ODAP and tannin contents (r = -0.379, P B 0.05) and a positive correlation (r = 0.843, P B 0.01) between a tannin content and flower color intensity were found. A white or crème colour was associated with a low tannin level in the seeds, while the colourful blooming accessions had higher tannin contents.

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“…While HPLC methods require laborious procedures for the derivatisation step, capillary zone electrophoresis allows selective and rapid measurements. The first capillary electrophoretic method developed for the separation and determination of a and b-ODAP seems to belong to Arentoftt and Greirson (1995), who used 20 mM Na 2 HPO 4 (pH 7.8) as running buffer solution, applied a constant voltage of 25 kV and kept the column temperature at 40°C. Zhao et al (1999) preferred to use 1 mM Na 2 B 4 O 7 /5 mM Na 2 SO 4 mixture as run buffer solution, and used H 3 PO 4 for pH adjustment to 9.20.…”

Section: Resultsmentioning

confidence: 99%

Homoarginine, β-ODAP, and asparagine contents of grass pea landraces cultivated in Turkey

et al. 2014

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Analysis of 3-(N-Oxalyl)-L-2,3-diaminopropanoic Acid and Its .alpha.-Isomer in Grass Pea (Lathyrus sativus) by Capillary Zone Electrophoresis

Cited by 32 publications

References 7 publications

Variability of phenotypic and morphological characteristics of some Lathyrus sativus L. and Lathyrus cicera L. accessions and nutritional traits of their seeds

Variability of phenotypic and morphological characteristics of some Lathyrus sativus L. and Lathyrus cicera L. accessions and nutritional traits of their seeds

Morphological characteristics of some accessions of grass pea (Lathyrus sativus L.) grown in Europe and nutritional traits of their seeds

Homoarginine, β-ODAP, and asparagine contents of grass pea landraces cultivated in Turkey

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