Analysis of 76 veterinary pharmaceuticals from 13 classes including aminoglycosides in bovine muscle by hydrophilic interaction liquid chromatography–tandem mass spectrometry

Determination of four main components of gentamicin in animal tissues after solid‐phase extraction by high‐performance liquid chromatography/tandem mass spectrometry

“…During the entire sample preparation process, the use glassware must be avoided since gentamicin is a kind of aminoglycoside antibiotic composed of amino sugars and amino cyclitols, both of which include many hydroxy groups that would form hydrogen bonds with silicon hydroxyl groups on the surface of the glass. Gentamicin extraction has most often been done by acetonitrile/water, hydrochloric acid, 10 mmol/L phosphate buffer (including 0.4 mmol/L EDTA) and 5% TCA, 2% perchloric acid (including 0.4 mmol/L EDTA and 10 mmol/L monopotassium phosphate) and 10 mmol/L ammonium acetate buffer (including 2% TCA, 0.4 mmol/L EDTA and 1% NaCl) . Gentamicin can be extracted from animal tissues under a high or a low pH .…”

“…The cleanup of gentamicin from animal matrix has been reported using C 18 , HLB, strong cation‐exchange SPE and weak cation‐exchange SPE. The reversed‐phase C 18 and HLB SPE methods must use an ion‐pairing agent to ensure retention of gentamicin, and the residue of the ion‐pairing agent in analytical samples may contaminate the ESI source and cause a loss in sensitivity in the negative ion mode.…”

MS-Based Analytical Techniques: Advances in Spray-Based Methods and EI-LC-MS Applications

“…Since 2010, numerous researches have used this methodology to detect pesticides in several fruits and vegetables, such as tomato [ 134 – 137 ], orange [ 138 ], mandarin [ 139 ], rice and red pepper [ 139 – 141 ], avocado [ 142 , 143 ], apples and cucumbers [ 144 , 145 ], mango [ 146 ], tea [ 147 ], lettuce [ 148 ], grains and cereals [ 149 – 152 ], soybean products [ 153 ], groundnut oil [ 154 ], and wines [ 155 ]. The same methodology was also used in the identification and quantification of veterinary drug residues in shellfish [ 156 ], meat [ 157 , 158 ], eggs and milk [ 158 ], and contaminants from food contact materials [ 159 ].…”

Dual‐layer column filtration cleanup and gas chromatography‐tandem mass spectrometry detection for the analysis of 39 pesticide residues in porcine meat

“…Owing to the high fat content and other components with large molecular weight, meat is regarded as a complex matrix. These interferences were expected to be excluded from the analyte solution to avoid significant matrix effects (MEs), or causing positive/negative qualitative analysis in chromatography separation followed by MS detection . Conventionally, SPE cleanup combined with freezing‐out or hydrophobic solvent exchange is necessary to remove the fatty lipids in meat extracts .…”