Analysis of a 36·2 kb DNA sequence including the right telomere of chromosome VI fromSaccharomyces cerevisiae

Abstract

The nucleotide sequence of a 36·2‐kb distal region containing the right telomere of chromosome VI was determined. Both strands of DNA cloned into cosmid clone 9965 and plasmid clone pEL174P2 were sequenced with an average redundancy of 7·9 per base pair, by both dye primer and dye terminator cycle sequencing methods. The G + C content of the sequence was found to be 37·9%. Eighteen open reading frames (ORFs) longer than 100 amino acids were detected. Four of these ORFs (9965orfR017, 9965orfF016, 9965orfR009 and 9965orfF003) were found to encode previously identified genes (YMR31, PRE4, NIN1 and HXK1, respectively). Six ORFs (9965orfR013, 9965orfF018, 9965orfF006, 9965orfR014, 9965orfF013 and 9965orfR020) were found to be homologous to hypothetical 121·4‐kDa protein in the BCK 5′ region, Bacillus subtilis DnaJ protein, hypothetical Trp‐Asp repeats containing protein in DBP3‐MRPL27, putative mitochondrial carrier YBR291C protein, Salmonella typhimurium nicotinate‐nucleotide pyrophosphorylase, and Escherichia coli cystathionine β‐lyase, respectively. The putative proteins encoded by 9965orfF018, 9965orfR014 and 9965orfR020 were found to be, respectively, a new member of the family of DnaJ‐like proteins, the mitochondrial carrier protein and cystathionine lyase. The nucleotide sequence reported here has been deposited in the DDBJ/GenBank/EMBL data library under Accession Number D44597.

Mitochondrial ribosomal proteins (MRPs) of yeast

“…p This name is preliminary, since no 2D PAGE position according to [11] has yet been established. [42,62] assume that at least 10 more MRPs remain to be found in the large subunit. Taken together, the yeast mitoribosome appears to contain at least 90 proteins, and the actual number may exceed 100.…”