Analysis of a 9.6 kb sequence from the 3' end of canine coronavirus genomic RNA

Horsburgh, Brian C.; Brierley, Ian; Brown, T. D. K.

doi:10.1099/0022-1317-73-11-2849

Cited by 65 publications

(90 citation statements)

References 44 publications

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“…More than 10 coronavirus N genes have now been sequenced, including those of HCV 229E, TGEV, PRCV, FIPV, CCV, murine hepatitis virus (MHV), HCV OC43, bovine coronavirus (BCV) and infectious bronchitis virus (IBV) (Schreiber et al, 1989;Kapke & Brian, 1986;Britton et al, 1991 ;Vennema et al, 1991 ;Horsburgh et al, 1992;Parker & Masters, 1990;Kamahora et al, 1989;Lapps et al, 1987;Boursnell et al, 1985). Many of these viruses have been sequenced by more than one group; the references quoted here were those used in the sequence analyses shown in Fig.…”

Section: Discussionmentioning

confidence: 99%

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Bridgen

Duarte

Tobler

et al. 1993

Journal of General Virology

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The nucleotide sequence of 1.7 kbp cDNA, comprising the region nearest the 3' end of the genome of the porcine epidemic diarrhoea virus (PEDV), has been independently determined for two European isolates of PEDV. Almost identical results were obtained for the two isolates, which were derived from cases of PEDV infection in Belgium and Britain in 1977 and 1987, respectively. The sequences contained a 1323 nucleotide (nt) open reading frame (ORF), which showed moderate identity to the nucleocapsid (N) gene of other coronaviruses. The greatest similarity at both the nucleic acid and protein levels was to the human coronavirus 229E.The PEDV N gene was, however, notably larger than that of the human 229E and porcine transmissible gastroenteritis viruses. This reflects the presence of a putative insertion of approximately 135 nt located towards the middle of the N gene. A second 336 nt ORF, which might encode a leucine-rich protein similar to, but shorter than, the bovine coronavirus internal protein was found within the PEDV N gene. Several RNA motifs typical of coronaviruses were also observed. These results confirm the earlier provisional classification of PEDV as a coronavirus.

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Section: Discussionmentioning

confidence: 99%

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Bridgen

Duarte

Tobler

et al. 1993

Journal of General Virology

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“…The S protein of strain CB/05 had the highest identity to FCoV type II strain 79-1683 (Figure 2). Comparison with strain CB/05 was possible only with CCoV type II strains Insavc-1 ( 10 ) and BGF ( 11 ) and CCoV type I strains Elmo/02 and 23/03 ( 3 , 12 ) because of a lack of data on the 3´ end of the CCoV genome in the genes encoding for nonstructural proteins (NSPs) 3a, 3b, 3c, 7a, and 7b. NSPs 3a, 7a, and 7b were not altered.…”

Section: The Studymentioning

confidence: 99%

Canine Coronavirus Highly Pathogenic for Dogs

Buonavoglia

Decaro

Martella

et al. 2006

Emerg. Infect. Dis.

180

176

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“…Ten µl RNA, 50 mM KCl, 10 mM Tris-HCl (pH 8), 400 µM dNTP, and 10 units RNAse inhibitor were added to a reaction mixture. CCV RNA was detected by semi-nested RT-PCR using three primers created from the 3' end of spike gene of CCV strain Insavc-1 (Horsburgh et al, 1992). In the first amplification step, 2 µl cDNA sample were amplified in a 50 µl reaction volume containing 50 mM KCl, 1.5 mM MgCl 2 , 10 mM Tris-HCl (pH 8), 200 µM dNTP, 1.5 units Taq DNA polymerase (QIAGEN, Germany) and 15 pM each of outer sense primer; CCV1 (located at position 1691: 5' GGC GTA ACT GAT GGA CCA CG 3') and outer anti-sense primer; CCV2 (located at position 2451: 5' CTT GTA CGG GCG GCA ACA TC 3').…”

Section: Reverse Transcription-polymerase Chain Reaction Detection Ofmentioning

confidence: 99%

Prevalence of canine coronavirus and parvovirus infections in dogs with gastroenteritis in Thailand

Sakulwira¹,

Vanapongtipagorn²,

Theamboonlers³

et al. 2003

Vet. Med. - Czech

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Canine coronavirus (CCV) and canine parvovirus type 2 (CPV-2) are the causative agents of gastroenteritis in dogs. Seventy fecal samples from dogs with signs of gastroenteritis (vomiting and diarrhea), twenty-five fecal samples from healthy dogs and one CPV-2 vaccine strain were amplified by semi-nested polymerase chain reaction (PCR) and semi-nested reverse transcriptase polymerase chain reaction (RT-PCR), aimed at specifically studying the gene encoding the most abundant capsid protein VP2 of CPV-2 and spike protein of CCV. The specificity of the CCV RT-PCR product was evaluated by sequencing. Positive specimens comprised 44 samples (62.8%) and 9 samples (12.8%) for CPV-2 and CCV, respectively. In nine CCV positive samples, seven displayed co-infection between CCV and CPV-2. Our CCV sequence (AF482001) showed a 94.9% nucleotide identity to CCV reported in GenBank accession number D13096. High prevalence of CCV and CPV-2 infections was found in 1–2 month- and 3–6 month-old dogs, respectively. Molecular biology of these viruses is important primarily for epidemic control and preventive measures.

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Analysis of a 9.6 kb sequence from the 3' end of canine coronavirus genomic RNA

Cited by 65 publications

References 44 publications

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Sequence determination of the nucleocapsid protein gene of the porcine epidemic diarrhoea virus confirms that this virus is a coronavirus related to human coronavirus 229E and porcine transmissible gastroenteritis virus

Canine Coronavirus Highly Pathogenic for Dogs

Prevalence of canine coronavirus and parvovirus infections in dogs with gastroenteritis in Thailand

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