Analysis of a dispersed repetitive DNA sequence in isogenic lines of Drosophila

Pierce, Dorothy A.; Lucchesi, John C.

doi:10.1007/bf00295007

Cited by 32 publications

(12 citation statements)

References 34 publications

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“…This confirms previous observations of other Drosophila populations on the same and other mobile elements (Pierce and Lucchesi, 1981;Montgomery and Langley, 1983;Belyaeva et aL, 1984;Ronsseray and Anxolabéhère, 1986).…”

Section: Discussionsupporting

confidence: 91%

Localisation polymorphism of mdg-1, copia, I and P mobile elements in genomes of Drosophila melanogaster, from data of inbred lines

Biémont

Gautier

1988

Heredity

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Using data from in situ hybridisation of the giant chromosomes from salivary glands, mdg-1, I, copia, and P mobile element polymorphism was studied in 17 highly-inbred lines of Drosophila melanogaster and in the expected hybrids obtained from the theoretically-crossed inbred lines. The mean copy numbers of each element on the inbred lines were close and equal to 16-8 for mdg-1, 17•5 for I, 178 for copia and 186 for P (these values included the insertions in the centromeric regions). The P element differed from the other three for variance in copy number and distribution of the number of insertion site occurrences. A low frequency of X-linked copia element insertions (as compared with frequency of insertions in the other chromosomes) was reported, suggesting that natural selection acts against insertional deleterious mutations of this element. Such a low frequency of X-linked insertions was not observed for the other three elements. The mdg-1, I and copia element copy numbers were positively correlated, thus leading to lines with high or low copy number. No correlation was observed between the P element copy number and the numbers of mdg-1, I and copia elements. Moreover, a genomic control of copy number was detected for mdg-1, I and copia, but not for P. The number of labelling sites, the number of copies, the number of heterozygous insertion sites, and various indices of genetic variability were estimated from the expected hybrids obtained from the theoretically-crossed inbred lines; the calculated numbers of labelling sites for mdg-1 and I did not differ from those obtained directly from the initial wild-derived population (we had no estimate of the number of labelling sites available in this population for copia and P). An average proportion of heterozygous sites of 086, 092, 0•90 and 094, and an average number of copies of 33, 35, 38 and 37 were found in the theoretical hybrids for the mdg-1, I, copia and P elements, respectively.

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Section: Discussionsupporting

confidence: 91%

Localisation polymorphism of mdg-1, copia, I and P mobile elements in genomes of Drosophila melanogaster, from data of inbred lines

Biémont

Gautier

1988

Heredity

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“…The division 42B for example is very impressive. All four nomadic segments of Young & Schwartz (1981), the four elements mdg-1, I, copia and P in the study of and many other sequences (297, 412, copia, mdg-I, mdg-3, roo;see Potter et al, 1979;Strobel et al, 1979;Pierce & Lucchesi, 1981;Ananiev et al, 1984;Belyaeva et aL, 1984;Birmont, 1986;Bi~mont & Aouar, 1987;Leigh Brown & Moss, 1987;Montgomery et al, 1987; hybridize to this chromosomal region.…”

Section: The Regions Of Insertionmentioning

confidence: 99%

Population genetics of transposable DNA elements

Biémont

1992

Genetica

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This paper is an attempt to bring together the various, dispersed data published in the literature on insertion polymorphism of transposable elements from various kinds of populations (natural populations, laboratory strains, isofemale and inbred lines). Although the results deal mainly with Drosophila, data on other organisms have been incorporated when necessary to illustrate the discussion. The data pertinent to the regions of insertion, the rates of transposition and excision, the copy number regulation, and the degree of heterozygosity were analysed in order to be confronted with the speculations made with various theoretical models of population biology of transposable elements. The parameters of these models are very sensitive to the values of the transposable element characteristics estimated on populations, and according to the difficulties of these estimations (population not at equilibrium, particular mutations used to estimate the transposition and excision rates, trouble with the in situ technique used to localize the insertions, undesired mobilization of TEs in crosses, spontaneous genome resetting, environmental effects, etc.) it cannot be decided accurately which model better accounts for the population dynamics of these TEs. Tendencies, however, emerge in Drosophila: the copia element shows evidence for deficiency of insertions on the X chromosomes, a result consistent with selection against mutational effects of copia insertions; the P element repartition does not significantly deviate from the neutral assumption, in spite of a systematic copy number of insertions higher on the X than on the autosomes. Data on other elements support either the neutral model of TE containment, neither of the two models, or both. Prudence in conclusion should then be de rigueur when dealing with such kind of data. Finally the potential roles of TEs in population adaptation and evolution are discussed.

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“…We also observed hobo insertion in division 42B on the 2R arm; the four elements mdg-1, I, copia and P in the study of and many other sequences (297, 412, mdg-3, roo) hybridize to this chromosomal region (Potter et al, 1979;Strobel, Dunsmuir & Rubin, 1979;Pierce & Lucchesi, 1981;Ananiev et al, 1984;Belyaeva, Ananiev & Gvozdev, 1984;Bi6mont & Aouar, 1987;Leigh-Brown & Moss, 1987;Montgomery, Charlesworth & Langley, 1987;Bi6mont & Gautier, 1989). It is thus likely that some regions of the genome have particular chromosomal environments (DNA sequence, local chromatin structure) that are more suitable for the insertion of different elements (Bi6mont, 1992).…”

Section: Discussionmentioning

confidence: 72%

Invasion of thehobo transposable element studied byin situ hybridization on polytene chromosomes ofDrosophila melanogaster

et al. 1994

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The invasion kinetics of hobo transposable element in the Drosophila melanogaster genome was studied by in situ hybridization on the polytene chromosomes. Six independent lines of Drosophila melanogaster flies that had been previously transformed by microinjection of the pHFL1 plasmid containing a complete hobo element were followed over 50 generations. We observed that hobo elements were scattered on each of the chromosome arms, with more insertion sites on the 3R arm. The total number of insertion sites remains quite small, between four and six, at generation 52. On the 2R arm, a short inversion appeared once at generation 52. Most of the integration sites reported here were already described for several transposons but some of them appear to be hotspots for hobo elements.

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Analysis of a dispersed repetitive DNA sequence in isogenic lines of Drosophila

Cited by 32 publications

References 34 publications

Localisation polymorphism of mdg-1, copia, I and P mobile elements in genomes of Drosophila melanogaster, from data of inbred lines

Localisation polymorphism of mdg-1, copia, I and P mobile elements in genomes of Drosophila melanogaster, from data of inbred lines

Population genetics of transposable DNA elements

Invasion of thehobo transposable element studied byin situ hybridization on polytene chromosomes ofDrosophila melanogaster

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