Analysis of a Leptospira interrogans locus containing DNA replication genes and a new IS, IS1502

Zuerner, Richard L.

doi:10.1016/s0378-1097(02)00935-7

Cited by 4 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…L. interrogans genome data varied widely from pathogenic spirochetes, Treponema pallidum and Borrelia burgdorferi, although they show considerable similarities in the genes that are responsible for their unique morphological features. Comparative genomics revealed both overall genetic similarities and significant structural differences, including a large chromosomal inversion and extensive variation in the number and distribution of insertion sequence elements, thus confirming genome plasticity as suggested by several previous studies [161,170,171,180,181]. The genomes of L. borgpetersenii showed approximately 700 kb reduction compared to L. interrogans and revealed substantial differences in genetic content and organization.…”

Section: Whole Genome Sequencingsupporting

confidence: 84%

Molecular Approaches in the Detection and Characterization of Leptospira

Ahmed¹,

Grobusch²

2012

J Bacteriol Parasitol

View full text Add to dashboard Cite

Conventional diagnosis of leptospirosis and characterization of Leptospira spp. relies mainly on serology. A major drawback of serology in diagnosis is that it needs sufficient levels of anti-Leptospira antibodies, thus jeopardizing confirmation in the early acute phase of disease. The cross agglutinin absorption test (CAAT) that determines Leptospira serovars is a technically demanding and laborious method and therefore is only performed at a few laboratories. Novel molecular diagnostic tests mainly rely on the polymerase chain reaction (PCR). PCR perfectly complements serological testing, since it is especially sensitive in the first 5 days after onset of symptoms. Real time PCR is rapid and has been validated for their high clinical accuracy. The introduction of molecular techniques for defining Leptospira species revolutionized the categorization of strains in this genus, as species and serogroups appeared to show little correlation. The reference test in molecular speciation is based on determining DNA homology. This approach is tedious and user-unfriendly and therefore is increasingly replaced by other techniques. To date, a wealth of molecular typing methods is available. Most attractive are those techniques that provide direct digital and electronically portable data. Such techniques comprise fluorescent amplified fragment length polymorphism (FAFLP), array typing, multilocus variable number of tandem repeats analysis (MLVA) and sequence-based phylogeny, and to some extent pulsed field gel electrophoresis (PFGE). Multilocus sequence typing (MLST) is the most robust method for determining Leptospira strain diversity and in the future will probably only be surpassed by phylogeny on whole genome sequences.

show abstract

Section: Whole Genome Sequencingsupporting

confidence: 84%

Molecular Approaches in the Detection and Characterization of Leptospira

Ahmed¹,

Grobusch²

2012

J Bacteriol Parasitol

View full text Add to dashboard Cite

show abstract

“…IS1500 (3) is equally distributed in both genomes (eight copies), the only difference being that in serovar Lai one copy invaded the CII replicon, which has not previously been described. Reminiscent insertions of IS1533 (51) and IS1502 (52) are found at the same relative locations in both genomes.…”

Section: Resultsmentioning

confidence: 90%

Comparative Genomics of Two Leptospira interrogans Serovars Reveals Novel Insights into Physiology and Pathogenesis

et al. 2004

View full text Add to dashboard Cite

Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in accidental hosts, including humans. Complete genome sequencing of Leptospira interrogans serovar Copenhageni and comparative analysis with the available Leptospira interrogans serovar Lai genome reveal that despite overall genetic similarity there are significant structural differences, including a large chromosomal inversion and extensive variation in the number and distribution of insertion sequence elements. Genome sequence analysis elucidates many of the novel aspects of leptospiral physiology relating to energy metabolism, oxygen tolerance, two-component signal transduction systems, and mechanisms of pathogenesis. A broad array of transcriptional regulation proteins and two new families of afimbrial adhesins which contribute to host tissue colonization in the early steps of infection were identified. Differences in genes involved in the biosynthesis of lipopolysaccharide O side chains between the Copenhageni and Lai serovars were identified, offering an important starting point for the elucidation of the organism's complex polysaccharide surface antigens. Differences in adhesins and in lipopolysaccharide might be associated with the adaptation of serovars Copenhageni and Lai to different animal hosts. Hundreds of genes encoding surface-exposed lipoproteins and transmembrane outer membrane proteins were identified as candidates for development of vaccines for the prevention of leptospirosis.

show abstract

“…Our analysis of the L. interrogans genome revealed the presence of the previously described insertion sequence (IS) elements IS1500, IS1501 (58), reminiscent insertions of IS1533 (59) and IS1502 (60) and an IS recently identified that we designated ISlin1 (5) ( Table 2). IS1500, IS1501 and ISlin1 account for 24 insertions distributed throughout chromosome I.…”

Section: Comparative Genomics and Insertion Sequencesmentioning

confidence: 88%

Genome features of Leptospira interrogans serovar Copenhageni

Nascimento

Verjovski-Almeida

Sluys

et al. 2004

Braz J Med Biol Res

203

183

View full text Add to dashboard Cite

We report novel features of the genome sequence of Leptospira interrogans serovar Copenhageni, a highly invasive spirochete. Leptospira species colonize a significant proportion of rodent populations worldwide and produce life-threatening infections in mammals. Genomic sequence analysis reveals the presence of a competent transport system with 13 families of genes encoding for major transporters including a three-member component efflux system compatible with the long-term survival of this organism. The leptospiral genome contains a broad array of genes encoding regulatory system, signal transduction and methyl-accepting chemotaxis proteins, reflecting the organism's ability to respond to diverse environmental stimuli. The identification of a complete set of genes encoding the enzymes for the cobalamin biosynthetic pathway and the novel coding genes related to lipopolysaccharide biosynthesis should bring new light to the study of Leptospira physiology. Genes related to toxins, lipoproteins and several surface-exposed proteins may facilitate a better understanding of the Leptospira pathogenesis and may serve as potential candidates for vaccine.

show abstract

Analysis of a Leptospira interrogans locus containing DNA replication genes and a new IS, IS1502

Cited by 4 publications

References 29 publications

Molecular Approaches in the Detection and Characterization of Leptospira

Molecular Approaches in the Detection and Characterization of Leptospira

Comparative Genomics of Two Leptospira interrogans Serovars Reveals Novel Insights into Physiology and Pathogenesis

Genome features of Leptospira interrogans serovar Copenhageni

Contact Info

Product

Resources

About