Analysis of adenoviruses and polyomaviruses quantified by qPCR as indicators of water quality in source and drinking-water treatment plants

Albinana-Gimenez, Nestor; Miagostovich, Marize Pereira; Calgua, Byron; Huguet, Josep Maria; Matía, L.; Gironés, Rosina

doi:10.1016/j.watres.2009.01.025

Cited by 148 publications

(93 citation statements)

References 40 publications

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“…Although many types of human AdV (HAdV) can be detected using cell culture, they vary with serotype and cell line and are laborious and time-consuming to measure in water (40). HAdV are readily detected and quantified in surface waters using PCR methods (40)(41)(42)(43). With molecular detection techniques, it is possible to detect nucleic acids of many human-pathogenic viruses, such as NoV, HAV, HEV, and others that are difficult or impossible to cultivate, although no conclusions about virus infectivity can be made from these techniques.…”

mentioning

confidence: 99%

Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors

Jones

Brassard

Topp

et al. 2017

Appl Environ Microbiol

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From the years 2008 to 2014, a total of 1,155 water samples were collected (spring to fall) from 24 surface water sampling sites located in a mixed-used but predominantly agricultural (i.e., dairy livestock production) river basin in eastern Ontario, Canada. Water was analyzed for viable F-specific DNA (F-DNA) and F-specific RNA (F-RNA) (genogroup I [GI] to GIV) coliphage and a suite of molecularly detected viruses (norovirus [GI to GIV], torque teno virus [TTV], rotavirus, kobuvirus, adenovirus, astrovirus, hepatitis A, and hepatitis E). F-DNA and F-RNA coliphage were detected in 33 and 28% of the samples at maximum concentrations of 2,000 and 16,300 PFU · 100 ml Ϫ1 , respectively. Animal TTV, human TTV, kobuvirus, astrovirus, and norovirus GIII were the most prevalent viruses, found in 23, 20, 13, 12, and 11% of samples, respectively. Viable F-DNA coliphage was found to be a modest positive indicator of molecularly detected TTV. F-RNA coliphage, unlike F-DNA coliphage, was a modest positive predictor of norovirus and rotavirus. There were, however, a number of significant negative associations among F-specific coliphage and viruses. F-DNA coliphage densities of Ͼ142 PFU · 100 ml Ϫ1 delineated conditions when ϳ95% of water samples contained some type of virus. Kobuvirus was the virus most strongly related to detection of any other virus. Land use had some associations with virus/F-specific coliphage detection, but season and surface water flow were the variables that were most important for broadly delineating detection. Higher relative levels of detection of human viruses and human F-RNA coliphage were associated with higher relative degrees of upstream human land development in a catchment.IMPORTANCE This study is one of the first, to our knowledge, to evaluate relationships among F-specific coliphages and a large suite of enteric viruses in mixed-use but agriculturally dominated surface waters in Canada. This study suggested that relationships between viable F-specific coliphages and molecularly detected viruses do exist, but they are not always positive. Caution should be employed if viable F-specific coliphages are to be used as indicators of virus presence in surface waters. This study elucidates relative effects of agriculture, wildlife, and human activity on virus and F-specific coliphage detection. Seasonal and meteorological attributes play a strong role in the detection of most virus and F-specific coliphage targets.KEYWORDS viruses, F-specific coliphages, pollution, indicators, surface water, microbial source tracking

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mentioning

confidence: 99%

Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors

Jones

Brassard

Topp

et al. 2017

Appl Environ Microbiol

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“…Recently, molecular techniques, specifically nucleic acid amplification procedures, immunocapturing, fluorescence in-situ hybridization (FISH), and polymerase chain reaction (PCR) have provided highly sensitive, rapid and quantitative analytical tools for detecting specific pathogens in environmental samples (Watson et al, 2004). These techniques are used to evaluate the microbiological quality of food and water, as well as microbial source-tracking (Albinana-Gimenez et al, 2009;Field et al, 2003;Hundesa et al, 2006). Most applied molecular techniques are based on protocols of nucleic acid amplification, of which the polymerase chain reaction (PCR) is the most commonly used.…”

Section: Methods Used In Detection Of Bacterial Pathogens In Watermentioning

confidence: 99%

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

Kinge¹,

Mbewe²,

Sithebe³

2012

Polymerase Chain Reaction

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“…Based on the different properties of the viruses, four main principal approaches are used for viral recovery and concentration. These include ultrafiltration, ultracentrifigation, and two-phase separation with polymers or flocculation, and adsorption-elution using filters, membranes, glass wool or glass powder [14,17,18].…”

Section: Rationale For Virological Analysis Of Watermentioning

confidence: 99%

“…The choice of the adsorbing matrix, eluting fluid and processing conditions are influenced by the nature of the sample. Adsorption and elution using charged membranes or filters is applied worldwide for the concentration of different enteric viruses and bacteriophages [18,19].…”

Section: Adsorption-elution Techniquesmentioning

confidence: 99%

“…To avoid loss of nucleic acids that can arise from chromatographic techniques and binding resins, bovine serum albumin (BSA) has been added to PCR mixtures to reduce inhibition by binding to inhibitors [8,11,39]. Methods including the use of internal or external controls are also available for identifying and monitoring inhibition [18,19,36,39]. Another approach to identify inhibition is to analyse shifts in the amplification efficiency of rt qRT-PCR/PCR reactions [33].…”

Section: Detection Of Viruses In Watermentioning

confidence: 99%

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Adsorption-Elution Techniques and Molecular Detection of Enteric Viruses from Water

Ruhanya

Kabego

Gichana

2016

JHVRV

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It is generally accepted by municipalities and public health authorities that monitoring microbial water quality is performed by enumerating faecal coliforms. These bacterial indicators of faecal contamination are used because of the assumption that they have the same behaviour in the water as enteric pathogens, which is not true with regards to viruses. Therefore, there is need for a supplementary viral indicator or to directly detect viruses from water. Detection of viruses in water is complicated because of occurrence of viruses in low numbers. There is need for concentration of the viruses from large volumes water to aliquots that can be used in PCR or cell culture. We present the adsorptionelution techniques for the recovery and concentration of enteric viruses from water and subsequent detection by quantitative real time RT-PCR/PCR.

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Analysis of adenoviruses and polyomaviruses quantified by qPCR as indicators of water quality in source and drinking-water treatment plants

Cited by 148 publications

References 40 publications

Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors

Waterborne Viruses and F-Specific Coliphages in Mixed-Use Watersheds: Microbial Associations, Host Specificities, and Affinities with Environmental/Land Use Factors

Detection of Bacterial Pathogens in River Water Using Multiplex-PCR

Adsorption-Elution Techniques and Molecular Detection of Enteric Viruses from Water

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