Analysis of Ah receptor pathway activation by brominated flame retardants

Brown, D. J. A.; Overmeire, Ilse Van; Goeyens, Léo; Denison, Michael S.; Vito, Michael J. De; Clark, George C.

doi:10.1016/j.chemosphere.2003.10.019

Cited by 83 publications

(34 citation statements)

References 36 publications

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“…Given the heterogenicity and variety of AhR ligands [179,180,181,182,183,184,185,186,193,194,196,197,198,199,200,201,202,203,204,205,206,207,208,209], using easily executed screening assays to identify its ligands only makes sense. Several in vitro test systems that screen for AhR ligands have been reported.…”

Section: Introductionmentioning

confidence: 99%

Potential Health-modulating Effects of Isoflavones and Metabolites via Activation of PPAR and AhR

2010

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Isoflavones have multiple actions on cell functions. The most prominent one is the activation of estrogen receptors. Other functions are often overlooked, but are equally important and explain the beneficial health effects of isoflavones. Isoflavones are potent dual PPARα/γ agonists and exert anti-inflammatory activity, which may contribute to the prevention of metabolic syndrome, atherosclerosis and various other inflammatory diseases. Some isoflavones are potent aryl hydrocarbon receptor (AhR) agonists and induce cell cycle arrest, chemoprevention and modulate xenobiotic metabolism. This review discusses effects mediated by the activation of AhR and PPARs and casts a light on the concerted action of isoflavones.

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Section: Introductionmentioning

confidence: 99%

Potential Health-modulating Effects of Isoflavones and Metabolites via Activation of PPAR and AhR

2010

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“…Polybrominated diphenyl ethers (PBDEs) are flame retardants commonly used in an array of products, including construction materials, electronics, furnishings, plastics, polyurethane foams, and textiles (Brown et al 2004). PBDEs are found in various environmental media (Bi et al 2006; Guan et al 2009; Thomsen et al 2010) and are health hazards.…”

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confidence: 99%

Effects of Decabrominated Diphenyl Ether (PBDE-209) in Regulation of Growth and Apoptosis of Breast, Ovarian, and Cervical Cancer Cells

Liu

Wang

et al. 2012

Environ Health Perspect

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Background: Polybrominated diphenyl ethers (PBDEs), commonly used in building materials, electronics, plastics, polyurethane foams, and textiles, are health hazards found in the environment.Objective: In this study we investigated the effects of PBDE-209, a deca-PBDE, on the regulation of growth and apoptosis of breast, ovarian, and cervical cancer cells as well as the underlying protein alterations.Methods: We used MCF-7 and MCF-7/ADR (multidrug-resistant MCF-7) breast cancer cell lines, the HeLa cervical cancer cell line, the OVCAR-3 ovarian cancer cell line, and the normal CHO (Chinese hamster ovary) cell line to assess the effects of PBDE-209 using cell viability, immunofluorescence, and flow cytometric assays. Western blot assays were used to detect changes in protein expression. To assess the effects of PBDE-209 on apoptosis, we used the protein kinase Cα (PKCα) inhibitor Gö 6976, the extracellular signal-regulated kinase (ERK) inhibitor PD98059, and tamoxifen.Results: Our data indicate that PBDE-209 increased viability and proliferation of the tumor cell lines and in CHO cells in a dose- and time-dependent manner. PBDE-209 also altered cell cycle distribution by inducing the S phase or G2/M phase. Furthermore, PBDE-209 partially suppressed tamoxifen-induced cell apoptosis in the breast cancer cell lines (MCF-7 and MCF-7/ADR) but suppressed Gö 6976- and PD98059-induced apoptosis in all cell lines. At the molecular level, PBDE-209 enhanced PKCα and ERK1/2 phosphorylation in the cell lines.Conclusions: Our data demonstrate that PBDE-209 is able to promote proliferation of various cancer cells from the female reproductive system and normal ovarian CHO cells. Furthermore, it reduced tamoxifen, PKCα, and ERK inhibition-induced apoptosis. Finally, PBDE-209 up-regulated phosphorylation of PKCα and ERK1/2 proteins in tumor cells and in CHO cells.

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“…1 Over the past three decades, PBDE concentrations in various biota and environmental media have doubled every four to six years and their congeners have been classified as highly toxic and recalcitrant environmental contaminants. 2 Hydroxylated PBDEs (OH-PBDEs) and methoxylated PBDEs (MeO-PBDEs) have also been detected in biological samples, such as marine organics, plants, and human blood, 3−5 and abiotic matrices, including air, surface and groundwater, sediment, and soil.…”

Section: ■ Introductionmentioning

confidence: 99%

Phytotoxicity of Brominated Diphenyl Ether-47 (BDE-47) and Its Hydroxylated and Methoxylated Analogues (6-OH-BDE-47 and 6-MeO-BDE-47) to Maize (Zea mays L.)

Huang

Wen

et al. 2015

Chem. Res. Toxicol.

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Polybrominated diphenyl ethers (PBDEs), methoxylated PBDEs (MeO-PBDEs), and hydroxylated PBDEs (OHPBDEs) are widely found in various environmental media, which is of concern given their biological toxicity. In this study, the phytotoxicities of BDE-47, 6-MeO-BDE-47, and 6-OH-BDE-47 to maize (Zea mays L.) were investigated by an in vivo exposure experiment. Results showed that BDE-47, 6-MeO-BDE-47, and 6-OH-BDE-47 inhibited seed germination and seedling development, and elevated malondialdehyde (MDA), carbonyl groups, and phosphorylated histone H2AX levels in maize roots, suggesting the inducement of lipid peroxidation, protein carbonylation, and DNA damage to maize. Exposure to BDE-47, 6-MeO-BDE-47, and 6-OH-BDE-47 caused the overproduction of H 2 O 2 , O 2•− , and •OH, and elevated the activities of antioxidant enzymes in the roots. In addition, 6-OH-BDE-47 caused more severe damage and reactive oxygen species (ROS) generation in maize than did BDE-47 and 6-MeO-BDE-47. These results demonstrated the phytotoxicities of BDE-47, 6-OH-BDE-47, and 6-MeO-BDE-47 to maize, and clarified that overproduction of ROS was the key mechanism leading to toxicity. This study offers useful information for a more comprehensive understanding of the environmental behaviors and toxicities of PBDEs, MeO-PBDEs, and OH-PBDEs.

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Analysis of Ah receptor pathway activation by brominated flame retardants

Cited by 83 publications

References 36 publications

Potential Health-modulating Effects of Isoflavones and Metabolites via Activation of PPAR and AhR

Potential Health-modulating Effects of Isoflavones and Metabolites via Activation of PPAR and AhR

Effects of Decabrominated Diphenyl Ether (PBDE-209) in Regulation of Growth and Apoptosis of Breast, Ovarian, and Cervical Cancer Cells

Phytotoxicity of Brominated Diphenyl Ether-47 (BDE-47) and Its Hydroxylated and Methoxylated Analogues (6-OH-BDE-47 and 6-MeO-BDE-47) to Maize (Zea mays L.)

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