Analysis of Aldo–Keto Reductase Gene Family and Their Responses to Salt, Drought, and Abscisic Acid Stresses in Medicago truncatula

Abstract

Salt and drought stresses are two primary abiotic stresses that inhibit growth and reduce the activity of photosynthetic apparatus in plants. Abscisic acid (ABA) plays a key role in abiotic stress regulation in plants. Some aldo–keto reductases (AKRs) can enhance various abiotic stresses resistance by scavenging cytotoxic aldehydes in some plants. However, there are few comprehensive reports of plant AKR genes and their expression patterns in response to abiotic stresses. In this study, we identified 30 putative AKR genes from Medicago truncatula. The gene characteristics, coding protein motifs, and expression patterns of these MtAKRs were analyzed to explore and identify candidate genes in regulation of salt, drought, and ABA stresses. The phylogenetic analysis result indicated that the 52 AKRs in Medicago truncatula and Arabidopsis thaliana can be divided into three groups and six subgroups. Fifteen AKR genes in M. truncatula were randomly selected from each group or subgroup, to investigate their response to salt (200 mM of NaCl), drought (50 g·L−1 of PEG 6000), and ABA (100 µM) stresses in both leaves and roots. The results suggest that MtAKR1, MtAKR5, MtAKR11, MtAKR14, MtAKR20, and MtAKR29 may play important roles in response to these stresses.

Novel virocell metabolic potential revealed in agricultural soils by virus‐enriched soil metagenome analysis

“…Other AMGs having key roles in plant health were also observed. For example, the Aspartyl and Cysteine proteases have a role in biocontrol activity (Liu and Yang, 2007; Harrison and Bonning, 2010; Deng et al ., 2018; Yamaya‐Ito et al ., 2018; Wang et al ., 2019) while the aldo/keto reductase increases protection against biotic and abiotic stress (Turóczy et al ., 2011; Yu et al ., 2020). The lytic effect of viruses targeting pathogenic rhizobacteria as well as their impact on plant‐associated microbial community assembly has been reported (Buttimer et al ., 2017; Morella et al ., 2018), although viruses may contribute to rhizosphere processes by multiple mechanisms yet to be uncovered.…”

“…Biocontrol activity (Harrison and Bonning, 2010) ASFRV147 Unknown Aldo/keto reductase, SCO4109 family Increase protection against abiotic stress (Turóczy et al, 2011;Yu et al, 2020) ASFRV202 Rhizobium…”

Genome-Wide Identification and Analysis of the Polycomb Group Family in Medicago truncatula

“…Promoters contain important regions that regulate gene expression. The response patterns of the target genes to the external environment can be predicted by analyzing the types of cis-acting elements involved [ 106 , 107 ]. To further study the regulatory mechanism of M. truncatula PcG genes in the processes of growth, development, and environmental stress, we predicted and analyzed the cis-acting elements in the promoter region.…”

Mapping Resistance to Argentinean Fusarium (Graminearum) Head Blight Isolates in Wheat

“…AKRs (KR18A1 gene) have been reported as promising agents for the control of Fusarium pathogens and detoxification of mycotoxins in plants and in food/feed products [52]. More recently, the role of AKRs has also been highlighted in salt, drought and abscisic acid stresses in other plant species [53]. Two more genes associated with FI_Type_II_2009 QTL on chromosome 3D were EEF1A lysine methyltransferase 2-like and cell division cycle protein 123 homolog.…”