Analysis of amines in plant materials

Bouchereau, Alain; Guénot, Pierre; Larher, F.

doi:10.1016/s0378-4347(00)00286-3

Cited by 95 publications

(46 citation statements)

References 182 publications

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“…However, fluorescent and UV monitoring obliges to fully resolve signal peaks since interfering compounds or derivatization side-products could be superimposed over the signal peaks of the polyamines. Mass spectrometry is widely recognized as a very reliable detection and characterization system and when, coupled with GC or HPLC separation, is probably the most powerful approach of analyte monitoring available (Boucherau et al, 2000). Although it is generally not necessary to fully resolve signal peaks when using mass spectrometry, a good separation will facilitate detection because of higher machine dwell time and higher signal-to-noise ratio's.…”

Section: A New Methods To Analyze Pavettamine and Other Polyaminesmentioning

confidence: 99%

Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont

Elst¹,

Wyk²,

Schultz³

et al. 2013

Phytochemistry

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Plant poisoning of livestock is responsible for considerable economic losses in southern Africa.Six plant species of the Rubiaceae family are known to cause gousiekte, a cardiac syndrome of ruminants induced by ingestion of the toxic compound pavettamine. Progress in understanding the aetiology of this disease is largely hampered by the variable toxicity of the plants and the -2 -absence of a quantification method for pavettamine. The pavettamine concentration in leaf samples of Fadogia homblei, a known gousiekte causing plant, was analyzed by massspectrometry. In the most apical leaf pair, the highest concentration of pavettamine was detected. Distal leaves contained progressively less pavettamine. Besides a significant amount of free pavettamine, most pavettamine was found to occur in a conjugated form. To which molecules the pavettamine is conjugated remains unknown as is the function of conjugated pavettamine in the development of gousiekte. All know gousiekte-causing plants contain symbiotic bacteria in their leaves; it was hypothesized that these bacteria might be involved in the production of pavettamine. However, analysis of in vitro cultures of the Fadogia homblei endosymbiont revealed no production of pavettamine. Pavettamine is therefore not produced by the bacteria alone. It is either the product of the interaction with the plant or solely produced by the host.

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Section: A New Methods To Analyze Pavettamine and Other Polyaminesmentioning

confidence: 99%

Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont

Elst¹,

Wyk²,

Schultz³

et al. 2013

Phytochemistry

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“…This has been achieved by freeze clamping, immediate freezing in liquid nitrogen, or by acidic treatments using perchloric or nitric acid (ap Rees and Hill, 1994). Although advantageous for extraction of amines (Bouchereau et al, 2000), acidic treatments pose severe problems for many analytical methods that follow, and harvesting and depositing plant tissues into tubes for liquid nitrogen freezing may take up to 15 s. Compared to freeze clamping techniques, freezing samples in liquid nitrogen is therefore a slower process that could potentially produce artefacts caused by wound responses, rapid activation of touch-inducible genes, etc. This might especially be the case if samples are weighed before freezing, and if no attention is paid to this special problem.…”

Section: Metabolomic Sample Preparationmentioning

confidence: 99%

Metabolomics — the link between genotypes and phenotypes

Fiehn

2002

Functional Genomics

1,625

1,337

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Metabolites are the end products of cellular regulatory processes, and their levels can be regarded as the ultimate response of biological systems to genetic or environmental changes. In parallel to the terms 'transcriptome' and 'proteome', the set of metabolites synthesized by a biological system constitute its 'metabolome'. Yet, unlike other functional genomics approaches, the unbiased simultaneous identification and quantification of plant metabolomes has been largely neglected. Until recently, most analyses were restricted to profiling selected classes of compounds, or to fingerprinting metabolic changes without sufficient analytical resolution to determine metabolite levels and identities individually. As a prerequisite for metabolomic analysis, careful consideration of the methods employed for tissue extraction, sample preparation, data acquisition, and data mining must be taken. In this review, the differences among metabolite target analysis, metabolite profiling, and metabolic fingerprinting are clarified, and terms are defined. Current approaches are examined, and potential applications are summarized with a special emphasis on data mining and mathematical modelling of metabolism.

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“…These methods have been widely used in the extraction of metabolites from animal and plant tissues (Shryock et al, 1986;Kopka et al, 1995;Chen et al, 1998;Adams et al, 1999;Bouchereau et al, 2000;Conneely et al, 2002) and microorganisms (Cordeiro and Freire, 1996;Weuster-Botz, 1997;Hajjaj et al, 1998;Schaefer et al, 1999;Vaseghi et al, 1999;Letisse and Lindley, 2000;Martins et al, 2001;Buchholz et al, 2002;Buziol et al, 2002;Maharjan and Ferenci, 2003). Acidic extraction is commonly used for the extraction of nucleotides and water-soluble metabolites (Vaseghi et al, 1999;Martins et al, 2001;Buchholz et al, 2002), but for metabolome studies it is evident that not all metabolites are stable at the extreme pH, and destruction of some nucleotides has been reported during acidic extractions (Hajjaj et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

“…Sample preparation for global metabolite analysis 1167 (Bouchereau et al, 2000), and the data acquisition method should be carefully chosen in order to eliminate the interferences of high salt concentrations. According to Maharjan and Ferenci (2003), destruction of compounds such as pyruvate, nucleotides and phosphorylated sugars under extreme acidic or alkaline pH is well documented.…”

mentioning

confidence: 99%

Global metabolite analysis of yeast: evaluation of sample preparation methods

Villas‐Bôas

Højer-Pedersen

Åkesson

et al. 2005

Yeast

366

363

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Sample preparation is considered one of the limiting steps in microbial metabolome analysis. Eukaryotes and prokaryotes behave very differently during the several steps of classical sample preparation methods for analysis of metabolites. Even within the eukaryote kingdom there is a vast diversity of cell structures that make it imprudent to blindly adopt protocols that were designed for a specific group of microorganisms. We have therefore reviewed and evaluated the whole sample preparation procedures for analysis of yeast metabolites. Our focus has been on the current needs in metabolome analysis, which is the analysis of a large number of metabolites with very diverse chemical and physical properties. This work reports the leakage of intracellular metabolites observed during quenching yeast cells with cold methanol solution, the efficacy of six different methods for the extraction of intracellular metabolites, and the losses noticed during sample concentration by lyophilization and solvent evaporation. A more reliable procedure is suggested for quenching yeast cells with cold methanol solution, followed by extraction of intracellular metabolites by pure methanol. The method can be combined with reduced pressure solvent evaporation and therefore represents an attractive sample preparation procedure for high-throughput metabolome analysis of yeasts.

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Analysis of amines in plant materials

Cited by 95 publications

References 182 publications

Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont

Production of toxic pavettamine and pavettamine conjugates in the gousiekte-causing Fadogia homblei plant and its relation to the bacterial endosymbiont

Metabolomics — the link between genotypes and phenotypes

Global metabolite analysis of yeast: evaluation of sample preparation methods

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