Analysis of amino acids in cell culture supernatant using capillary electrophoresis with contactless conductivity detection

Abstract

CE-C 4 D methods for the analysis of amino acids (AAs) are presented. Combining the results from two methods with acetic acid and cyclodextrin-based BGEs, 20 proteinogenic AAs could be analyzed using CE. CE-C 4 D was also, for the first time, applied to analyze free AAs in samples of mammalian cell culture supernatant. After dilution as only sample preparation, combining the results of the two CE methods allowed monitoring the concentration changes of 17 AAs in samples taken during the cultivation of CHO cells.

Overview of chromatographic and electrophoretic methods for the determination of branched‐chain amino acids

“…Even though the BCAAs separation was not fully achieved, it was compared with wet blood chemistry, and both were highly correlated [182]. A combination of two methods employing AcOH and cyclodextrin-based BGEs has been used to analyze 20 AAs using CE-C 4 D. Despite this, the individual methods did not achieve the separation of BCAAs [183].…”

Quantification of amino acids secreted by yeast cells by hydrophilic interaction liquid chromatography‐tandem mass spectrometry

“…Moreover, AAs play a crucial role in facilitating the growth and development of cells, the genesis of new cellular entities, and the preservation of preexisting cellular structures within the field of biochemistry. Of note, AAs are essential for coordinating the normal proliferation and maturation of cells [10]. As it pertains to the regulation of cell growth and metabolism, monitoring the levels of AAs in mammalian or bacterial cell cultures provides invaluable information [11], among others in biopharmaceutical development [12].…”