Analysis of an outbreak of staphylococcal scalded skin syndrome: strategies for typing ‘non-typable’ strains

Dowsett, E.G.; Petts, D N; Baker, Shiarron; DeSaxe, M.J.; Coe, Allan E.; Naidoo, Jay; Noble, W. C.

doi:10.1016/0195-6701(84)90007-0

Cited by 19 publications

(10 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Phage-typing was carried out at the Institute laboratories using the International set of phages and standard techniques, and also by the Division of Hospital Infection, Central Public Health Laboratory. Plasmid profiling was carried out as described in Dowsett et al (1984). Curing ofresistance was attempted by growing staphylococci at 42 'C with repeated subculture (Asheshov, 1966).…”

Section: Methodsmentioning

confidence: 99%

Transmissible mupirocin resistance inStaphylococcus aureus

1989

View full text Add to dashboard Cite

SUMMARYThe spread of two strains of Staphylococcus aureus with high level resistance to mupirocin is described. The resistance proved to be easily transferred to other S. aureus strains by filter mating experiments and on the skin of mice. No plasmid band corresponding to the resistance could be demonstrated by agarose gel electrophoresis or by caesium chloride gradient centrifugation but cleavage of 'chromosomal' DNA from resistant recipients showed bright bands of DNA absent from sensitive controls.

show abstract

Section: Methodsmentioning

confidence: 99%

Transmissible mupirocin resistance inStaphylococcus aureus

1989

View full text Add to dashboard Cite

show abstract

“…Plasmid profiles were constructed by lysing cells and separating chromosomal and plasmid DNA by gel electrophoresis (See Dowsett et al 1984 for details).…”

Section: Methodsmentioning

confidence: 99%

“…We have found this technique valuable in studies of the epidemiology of scalded skin syndrome (Dowsett et al 1984), in tracing methicillin resistant S. aureus (Cookson et al 1985) and for coagulase negative staphylococci in CAPD patients (Degener et al 1986). However in the absence ofother characteristics for typing strains its validity cannot be established.…”

Section: Introductionmentioning

confidence: 99%

Plasmid profiling of epidemic staphylococci from around 1960: a comparison of epidemiological techniques

Noble

Rahman

1986

J. Hyg.

View full text Add to dashboard Cite

SUMMARYPlasmid profiles have been established for 68 isolates of Staphylococcus aureus from 13 episodes of epidemic spread in hospital wards between 1958 and 1962. Despite the original lack of care in preservation of strains the profiles give, in general, the same epidemiological patterns as were established originally on the basis of phage type, antibiotic sensitivity, ward and date of isolation.

show abstract

“…In the first outbreak, a separate toxigenic strain typing weakly with 55 was recovered from an affected baby and a staff carrier. Heat treatment, in vivo toxin assays and plasmid profiles were needed by Dowsett and colleagues [19] to show that five babies yielding a toxigenic non-typable strain related to group III formed an outbreak. At the same time another strain typing 3A/3C/55/71 was recovered from an affected baby, a sibling and a staff member and several mother-baby pairs were detected.…”

Section: Discussionmentioning

confidence: 99%

“…Lawns of Corynebacterium renale NCTC 11140 [19] and S. aureus NCTC 10804 (502A [10]) were prepared on nutrient agar. Wells were cut out with a cork borer and filled with supernate from a centrifuged overnight broth culture of the test isolate.…”

Section: Reference Laboratory Methodsmentioning

confidence: 99%

A double outbreak of exfoliative toxin–producing strains ofStaphylococcus aureusin a maternity unit

Dave¹,

Reith

Nash

et al. 1994

Epidemiol. Infect.

View full text Add to dashboard Cite

SummaryThis report describes a double outbreak of staphylococcal scalded skin syndrome (SSSS) in which two distinct tetracycline-resistant strains ofStaphylococcus aureusproducing different exfoliative toxins were involved. In the first phase the daytime staff of the delivery unit and eczematous skin conditions in midwives were implicated as the probable source. In the second phase a source within a post-natal ward was suggested with local cross-infection. In the final phase both sources were epidemiologically linked to cases of SSSS. Because early discharge was the policy of the unit many cases presented in the community rather than in the hospital.Confirmation of epidemiological findings was provided by additional laboratory studies. Two distinct strains ofS. aureuscould be defined, differing in phage-typing patterns, the exfoliative toxin produced, plasmid profile, cadmium resistance and bacteriocin production. Strict care in hand washing with a chlorhexidine-containing detergent was an important control measure.

show abstract

Analysis of an outbreak of staphylococcal scalded skin syndrome: strategies for typing ‘non-typable’ strains

Cited by 19 publications

References 3 publications

Transmissible mupirocin resistance inStaphylococcus aureus

Transmissible mupirocin resistance inStaphylococcus aureus

Plasmid profiling of epidemic staphylococci from around 1960: a comparison of epidemiological techniques

A double outbreak of exfoliative toxin–producing strains ofStaphylococcus aureusin a maternity unit

Contact Info

Product

Resources

About