Analysis of aneuploidy rate in antral and ovulated mouse oocytes during female aging

Zuccotti, Maurizio; Boiani, Michele; Garagna, Silvia; Redi, Carlo Alberto

doi:10.1002/(sici)1098-2795(199807)50:3<305::aid-mrd6>3.0.co;2-n

Cited by 36 publications

(16 citation statements)

References 23 publications

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“…Aging is also correlated with an increase in the aneuploidy rate of oocytes (17)(18)(19)(20). Our study illustrates that the age of the female can perturb oocyte maturation, which is corroborated by the Jones study (20).…”

supporting

confidence: 84%

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Zhang

et al. 2011

Fertility and Sterility

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supporting

confidence: 84%

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Zhang

et al. 2011

Fertility and Sterility

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“…MII oocytes were processed for chromosome counting by air-drying and Hoechst dye staining, as described (Zuccotti et al 1998). As chromosome spreads with fewer than 20 chromosomes may be the result of chromosome loss during the spreading procedure, only supernumerary chromosomes are considered reliable.…”

Section: Karyotype Analysismentioning

confidence: 99%

Maternal age effect on mouse oocytes: new biological insight from proteomic analysis

Schwarzer¹,

Siatkowski²,

Pfeiffer³

et al. 2014

Reproduction

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The long-standing view of 'immortal germline vs mortal soma' poses a fundamental question in biology concerning how oocytes age in molecular terms. A mainstream hypothesis is that maternal ageing of oocytes has its roots in gene transcription. Investigating the proteins resulting from mRNA translation would reveal how far the levels of functionally available proteins correlate with mRNAs and would offer novel insights into the changes oocytes undergo during maternal ageing. Gene ontology (GO) semantic analysis revealed a high similarity of the detected proteome (2324 proteins) to the transcriptome (22 334 mRNAs), although not all proteins had a cognate mRNA. Concerning their dynamics, fourfold changes of abundance were more frequent in the proteome (3%) than the transcriptome (0.05%), with no correlation. Whereas proteins associated with the nucleus (e.g. structural maintenance of chromosomes and spindle-assembly checkpoints) were largely represented among those that change in oocytes during maternal ageing; proteins associated with oxidative stress/damage (e.g. superoxide dismutase) were infrequent. These quantitative alterations are either impoverishing or enriching. Using GO analysis, these alterations do not relate in any simple way to the classic signature of ageing known from somatic tissues. Given the lack of correlation, we conclude that proteome analysis of mouse oocytes may not be surrogated with transcriptome analysis. Furthermore, we conclude that the classic features of ageing may not be transposed from somatic tissues to oocytes in a one-to-one fashion. Overall, there is more to the maternal ageing of oocytes than mere cellular deterioration exemplified by the notorious increase of meiotic aneuploidy.

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“…Chromatin attains a so called "surrounded nucleolus" (SN) configuration within the GV of competent oocytes of most mammals including the human. When GV stage oocytes of mouse with a SN nuclear morphology are isolated from antral follicles past the time they are ready to resume maturation (preovulatory overmaturity) and are matured in vitro to metaphase II, they show increased aneuploidy compared to controls isolated from follicles at the appropriate time after stimulation of follicular development (unaged cohort) (Zuccotti et al, 1998). This suggests that prolonged transcriptional suppression and events prior to resumption of maturation may interfere with scheduled gene expression patterns, spindle formation and chromosome behaviour at maturation thus leading to meiotic errors.…”

Section: Relevance Of Altered Expression In Aged Oocytesmentioning

confidence: 99%

Oocyte ageing and its cellular basis

Eichenlaub-Ritter

2012

Int. J. Dev. Biol.

104

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Aneuploidy is extremely high in aged human oocytes. Its cellular origin has been elusive. Trisomy data implicate predominantly meiosis I errors in the genesis of oocyte aneuploidy. Susceptible recombination patterns increase risks for nondisjunction. Cytogenetic analyses of aged human oocytes and embryos from assisted reproduction (ART) suggest that aneuploidy primarily relates to precocious chromatid separation. Oocytes express a spindle assembly checkpoint (SAC), but do not arrest maturation in the presence of improperly attached or single, unattached chromosomes. The SAC may be more permissive by altered gene expression in aged oocytes. Aged oocytes frequently exhibit precocious loss of chromosome cohesion. In experimental models, cohesion cannot be restored once lost, a process possibly occurring during long meiotic arrest. Maternal age, hormonal stimulation, disturbed metabolism, and depletion of the follicle pool contribute to mitochondrial dysfunction, spindle aberrations, and errors in chromosome segregation. Caloric restriction and antioxidants reduce mitochondrial dysfunction and aneuploidy in aged rodent's oocytes. Loss of chromosome cohesion appears to be a major risk factor for aneuploidy by disturbing the sequential separation of homologs and chromatids. A permissive SAC, the presence of risky meiotic exchanges, changes in expression, and failures to resolve improper chromosome attachments, as well as mitochondrial dysfunction may synergistically increase susceptibility to meiotic errors. A healthy life style, mild stimulation and an optimal environment may delay ageing and sustain control over chromosome disjunction, whereas loss of cohesion appears to be irreversible.

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Analysis of aneuploidy rate in antral and ovulated mouse oocytes during female aging

Cited by 36 publications

References 23 publications

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Age-related changes in the localization of DNA methyltransferases during meiotic maturation in mouse oocytes

Maternal age effect on mouse oocytes: new biological insight from proteomic analysis

Oocyte ageing and its cellular basis

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