Abstract:The high frequency of infection by Anisakis simplex (A. simplex) has led to an increase in IgE sensitization, turning allergy to this parasite a relevant contemporary health problem. Improving the lack of conventional diagnosis test specificity is crucial to better understand these clinical scenarios. Specific IgE (sIgE) to A. simplex extract by ImmunoCAP (Anisakis-sIgE) was determined in sera from 403 blood donors (BD) from Cantabria (North of Spain) of which 51 subjects resulted sensitized. Among these latte… Show more
“…Regarding the results against the Ani s 1 allergen, no volunteer tested positive. These results are in accordance with the literature 16,30 , taking into account that a positive IgE response to Ani s 1 is mainly associated with symptomatic patients, and more specifically with allergic anisakiasis, a condition defined as an active infection by Anisakis larvae. These results are comparable with the seroprevalence reported in Norway 25 where, in a population with similar characteristics, all sera tested also negative to Ani s 1.…”
The anisakiasis is little known in Colombia and rarely diagnosed. However, reports of fish parasitized by anisakid larvae in South America are increasing. Objectives: The objective was determine the prevalence of sensitization to Anisakis simplex in a healthy population in Colombia Methods: Cross-sectional survey of 150 volunteers from Universidad del Valle’s community, Cali, Colombia. Past medical history, including eating habits associated with fish consumption was assessed. Prevalence of IgE antibodies was determined by the ELISA- Trisakis 170 kit for the recombinant allergens Ani s 1 and Ani s 7. Findings: Two sera (1.3%) of blood tested were positive to the Ani s 7 antigen, while for Ani s 1 all sera were negative. Conclusions: This research is the first exploratory study on Anisakis prevalence conducted in Colombia, the results do not indicate a major problem. Nevertheless, more epidemiological studies are needed.
“…Regarding the results against the Ani s 1 allergen, no volunteer tested positive. These results are in accordance with the literature 16,30 , taking into account that a positive IgE response to Ani s 1 is mainly associated with symptomatic patients, and more specifically with allergic anisakiasis, a condition defined as an active infection by Anisakis larvae. These results are comparable with the seroprevalence reported in Norway 25 where, in a population with similar characteristics, all sera tested also negative to Ani s 1.…”
The anisakiasis is little known in Colombia and rarely diagnosed. However, reports of fish parasitized by anisakid larvae in South America are increasing. Objectives: The objective was determine the prevalence of sensitization to Anisakis simplex in a healthy population in Colombia Methods: Cross-sectional survey of 150 volunteers from Universidad del Valle’s community, Cali, Colombia. Past medical history, including eating habits associated with fish consumption was assessed. Prevalence of IgE antibodies was determined by the ELISA- Trisakis 170 kit for the recombinant allergens Ani s 1 and Ani s 7. Findings: Two sera (1.3%) of blood tested were positive to the Ani s 7 antigen, while for Ani s 1 all sera were negative. Conclusions: This research is the first exploratory study on Anisakis prevalence conducted in Colombia, the results do not indicate a major problem. Nevertheless, more epidemiological studies are needed.
“…2 , this tryptic peptide shows 75% sequency identity (6/8) and 87.5% (7/8) similarity with two UA3-recognized sequences (i.e., P3 and the partial sequence of native Ani s 7.0101, respectively). Moreover, as Ani s 7 is the reported Anisakis specific allergen more frequently recognized by infected patients 20 – 22 , we investigated whether the mAb UA3 is able to recognize an Ani s 7-like molecule in P. decipiens s.s. preparations using a sandwich ELISA. Figure 2 Alignment of the Ani s 7.0101 precursor peptide YGIEFCNR detected in the Pseudoterranova decipiens s.s. excretory-secretory protein extract (ESP) with the amino acid sequence from the protein ABL77410.1 (partial Ani s 7.0101) and the amino acid sequence (P3), both reported to be recognized by mAb UA3 19 .…”
The family Anisakidae, mainly represented by Anisakis simplex s.l. and Pseudoterranova decipiens, encompasses zoonotic nematodes infecting many marine fish. Both are responsible for gastrointestinal disease in humans after ingestion of a live larva by consumption of undercooked fish, and, in the case of A. simplex, an allergic reaction may occur after consuming or even handling infected fish. Due to its phylogenetic relatedness with A. simplex, few studies investigated the allergenic potential of P. decipiens, yet none of them focused on its excretory/secretory (E/S) proteins that easily get missed when working solely on extracts from crushed nematodes. Moreover, these E/S allergens remain behind even when the larva has been removed during fish quality processing. Therefore, the aim was to investigate if Anisakis-like allergens could also be detected in both crushed and E/S P. decipiens protein extract using targeted mass spectrometry analysis and immunological methods. The results confirmed that at least five A. simplex allergens have homologous proteins in P. decipiens; a result that emphasizes the importance of also including E/S protein extracts in proteomic studies. Not only A. simplex, but also P. decipiens should therefore be considered a potential source of allergens that could lead to hypersensitivity reactions in humans.
“…in frozen raw fish fillets samples (N=384), collected in three markets in Medellin and its metropolitan area (Antioquia, Colombia). 1 Buenaventura, Bahía Solano, Santa Marta 2 Ecuador, Chile 3 Central Mayorista de Antioquia, Placita de Flórez 4 Salmo salar (salmon), Thunnus tuna (tuna), Merluccus merlusus (hake). 5 Positivity to parasites of the genus Anisakis spp., according to the visualization technique of larvae by means of the pressing method and ultraviolet light.…”
Introduction: Anisakiasis is a human parasitic disease caused by the consumption of raw fish or shellfish containing larvae of the Anisakidae family. It is currently considered an emerging disease of public health interest.Objective: To identify the presence of larvae of the Anisakidae family in samples of frozen raw fish fillets intended for human consumption in markets in Medellín and its metropolitan area in Antioquia, Colombia.Materials and methods: A cross-sectional study was carried out, in which larvae of the Anisakidae family were detected and identified in frozen raw fish fillets from three representative markets in Medellín and its metropolitan area. A total of 384 ready for consumption fillets were analyzed (197 sawfish, 137 salmon, 37 tuna, and 13 hake), using the pressing and ultraviolet light method. Taxonomic keys were used to identify the collected parasites and to establish its genus. Conventional PCR and Sanger sequencing was performed to determine the species.Results: Four larvae were found in 4 of the 384 (1.04%) fillets (CI95% 1.04 ± 1.01%). The species of fish in which the larvae were found was sawfish (Scomberomorus spp.) and the genus and species of the larvae was established as Anisakis pegreffii.Conclusions: According to the study, the presence of Anisakis parasites in frozen raw fish fillets in the influence area is evident.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
