Analysis of annexin-A1 in the macrophages and apoptotic cells of patients with cutaneous leishmaniasis

Pona, Mariana Nascimento; Dietrich, Jordhana Mendonça; Silva, Joselina Maria da; Silva, Helen Aguiar Lemes da; Hueb, Márcia; Damazo, Amílcar Sabino

doi:10.1590/0037-8682-0756-2020

Cited by 4 publications

(4 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Notably, most patients had ECR lesions, indicating early disease detection. Similar findings have been described previously 16 , 17 , 21 . These patients had uncomplicated CL and were treated with meglumine antimoniate, a drug used for CL treatment 1 , 22 , 23 .…”

Section: Discussionsupporting

confidence: 92%

“…Most of the patients were male, with diverse skin colors. Similar results have been previously described for this region 16 , 17 , 21 .…”

Section: Discussionsupporting

confidence: 91%

“…Additionally, ANXA1 can modulate important cytokines (e.g, TNF-α, IL-1β, and IL-10) 18 , potentially influencing differentiation towards M2 macrophages. Several studies have reported the presence of ANXA1 in the CL 16 , 17 , 21 . One of these works related to ANXA1 action in the phagocytic activity of neutrophils infected with L. braziliensis 21 .…”

Section: Discussionmentioning

confidence: 99%

“…Several studies have reported the presence of ANXA1 in the CL 16 , 17 , 21 . One of these works related to ANXA1 action in the phagocytic activity of neutrophils infected with L. braziliensis 21 . Another study identified the differentiation of ANXA1 expression in macrophages subtypes 16 .…”

Section: Discussionmentioning

confidence: 99%

See 3 more Smart Citations

Analysis of clinical cure outcome, macrophages number, cytokines levels and expression of annexin-A1 in the cutaneous infection in patients with Leishmania braziliensis

Silva,

Sarmento

et al. 2024

Rev. Soc. Bras. Med. Trop.

View full text Add to dashboard Cite

Background: Leishmania braziliensis , a protozoan prevalent in Brazil, is the known causative agent of cutaneous leishmaniasis (CL). The activation of M1 macrophages is a pivotal factor in the host's ability to eliminate the parasite, whereas M2 macrophages may facilitate parasite proliferation. This study analyzed the clinical outcomes of CL and the patients' immunological profiles, focusing on the prevalence of M1 and M2 macrophages, cytokine production, and annexin-A1 (ANXA1) expression in the lesion. Methods: Data were obtained by polymerase chain reaction (PCR) and histopathological, immunofluorescence, and cytokine analyses. Results: Patients with exudative and cellular reaction-type (ECR)-type lesions that healed within 90 days showed a significant increase in M1. Conversely, patients with ECR and exudative and granulomatous reaction (EGR)types, who healed within 180 days, showed an elevated number of M2. Cytokines interferon (IFN)-γ and tumor necrosis factor (TNF)-α were higher in ECR lesions that resolved within 90 days (P<0.05). In contrast, IL-9 and IL-10 levels significantly increased in both ECR and EGR lesions that healed after 180 days (P<0.001). The production of IL-21, IL-23 and TGF-β was increased in patients with ECR or EGR lesions that healed after 180 days (P<0.05). The expression of ANXA1 was higher in M2 within ECR-type lesions in patients who healed after 180 days (P<0.05). Conclusions: These findings suggest that the infectious microenvironment induced by L. braziliensis affects the differentiation of M1 and M2 macrophages, cytokine release, and ANXA1 expression, thereby influencing the healing capacity of patients. Therefore, histopathological and immunological investigations may improve the selection of CL therapy.

show abstract