Analysis of antennal transcriptome and odorant binding protein expression profiles of the recently identified parasitoid wasp, Sclerodermus sp.

Zhou, Chang-Xiang; Min, Shui-Fa; Tang, Yanting; Wang, Man‐Qun

doi:10.1016/j.cbd.2015.06.003

Cited by 30 publications

(38 citation statements)

References 51 publications

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“…Three conserved antennal IRs were previously found in the parasitoid wasp Sclerodermus sp. (Zhou, Bi, et al., ; Zhou, Min, et al., ). Our results showed that some IRs are missing in the antennal transcriptomes of both P. solenopsis and A. bambawalei .…”

Section: Discussionmentioning

confidence: 99%

“…In addition, there are only 10 predicted OBP genes in Sclerodermus sp. (Zhou, Bi, et al., ; Zhou, Min, et al., ) and 22 predicted OBP genes in Cotesia vestalis (Nishimura, Brillada, Yazawa, Maffei, & Arimura, ), while 90 OBP genes in N. vitripennis (Vieira et al., ). From the phylogenetic tree of candidate OBPs, we found that six PsolOBPs (PsolOBP1, 2, 3, 4, 5 and 8) were grouped in the same clusters with AbamOBPs.…”

Section: Discussionmentioning

confidence: 99%

“…High expression of OBPs in non‐olfactory tissues was also reported in other insect species including Sogatella furcifera (He & He, ), Sclerodermus sp. (Zhou, Min, et al., ) and Tenebrio molitor (Liu et al., ). These studies suggest that OBPs may have other non‐olfactory functions.…”

Section: Discussionmentioning

confidence: 99%

“…The behavioural response of A. bambawalei also varies widely among different host stages of P. solenopsis (He, Feng, Li, Zhou, & Xu, 2015;Zhang, Zhang, Wang, & Kong, 2014). Aenasius bambawalei is obviously attracted to plants damaged by P. solenopsis (Zhou, Bi, et al, 2015;Zhou, Min, Yan, & Wang, 2015), suggesting that chemical cues are involved in host location of A. bambawalei to P. solenopsis. Olfactory sensation may play an important role in mediating the interactions among three trophic levels.…”

Section: The Mealybug Phenacoccus Solenopsis Tinsley (Hemipteramentioning

confidence: 99%

See 3 more Smart Citations

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Nie

et al. 2017

J Applied Entomology

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Olfaction is fundamental to insect survival due to its roles in host location and interspecific communications. Here, we analysed the transcriptomes of odorant binding proteins (OBPs) from the invasive mealybug Phenacoccus solenopsis and its endopara- AbamOBP30 were expressed at high levels in male antenna. This study for the first time identified the chemosensory genes and determined their expression patterns in different tissues of the two species. Our results provide important molecular information for the exploration of chemosensory mechanisms in these two species.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: The Mealybug Phenacoccus Solenopsis Tinsley (Hemipteramentioning

confidence: 99%

See 2 more Smart Citations

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Nie

et al. 2017

J Applied Entomology

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“…The open reading frames (ORFs) and the associated amino acid sequences were determined by using GeneDoc 2.7.0 software. The phylogenetic tree for OBP and CSP was constructed based on MmedOBP/CSP sequences and orthologues in other Hymenoptera species [15, 27–29]. Amino acid sequences were aligned using the program Clustal X 2.0 [30].…”

Section: Methodsmentioning

confidence: 99%

Identification of odorant binding proteins and chemosensory proteins in Microplitis mediator as well as functional characterization of chemosensory protein 3

Peng

Wang

et al. 2017

PLoS ONE

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Odorant binding proteins (OBPs) and chemosensory proteins (CSPs) play important roles in transporting semiochemicals through the sensillar lymph to olfactory receptors in insect antennae. In the present study, twenty OBPs and three CSPs were identified from the antennal transcriptome of Microplitis mediator. Ten OBPs (MmedOBP11–20) and two CSPs (MmedCSP2–3) were newly identified. The expression patterns of these new genes in olfactory and non-olfactory tissues were investigated by real-time quantitative PCR (qPCR) measurement. The results indicated that MmedOBP14, MmedOBP18, MmedCSP2 and MmedCSP3 were primarily expressed in antennae suggesting potential olfactory roles in M. mediator. However, other genes including MmedOBP11–13, 15–17, 19–20 appeared to be expressed at higher levels in body parts than in antennae. Focusing on the functional characterization of MmedCSP3, immunocytochemistry and fluorescent competitive binding assays were conducted indoors. It was found that MmedCSP3 was specifically located in the sensillum lymph of olfactory sensilla basiconca type 2. The recombinant MmedCSP3 could bind several types of host insects odors and plant volatiles. Interestingly, three sex pheromone components of Noctuidae insects, cis-11-hexadecenyl aldehyde (Z11-16: Ald), cis-11-hexadecanol (Z11-16: OH), and trans-11-tetradecenyl acetate (E11-14: Ac), showed high binding affinities (Ki = 17.24–18.77 μM). The MmedCSP3 may be involved in locating host insects. Our data provide a base for further investigating the physiological roles of OBPs and CSPs in M. mediator, and extend the function of MmedCSP3 in chemoreception of M. mediator.

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Antennal transcriptome analysis of Psyttalia incisi (silvestri) (Hymenoptera: Braconidae): identification and tissue expression profiling of candidate odorant-binding protein genes

Yang,

Li,

Jiang

et al. 2024

Mol Biol Rep

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Analysis of antennal transcriptome and odorant binding protein expression profiles of the recently identified parasitoid wasp, Sclerodermus sp.

Cited by 30 publications

References 51 publications

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Antennal transcriptome and odorant binding protein expression profiles of an invasive mealybug and its parasitoid

Identification of odorant binding proteins and chemosensory proteins in Microplitis mediator as well as functional characterization of chemosensory protein 3

Antennal transcriptome analysis of Psyttalia incisi (silvestri) (Hymenoptera: Braconidae): identification and tissue expression profiling of candidate odorant-binding protein genes

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