Analysis of anthocyanins and flavonols in petals of 10 Rhododendron species from the Sygera Mountains in Southeast Tibet

Liu, Lin; Zhang, Liangying; Wang, Shuli; Niu, Xinyu

doi:10.1016/j.plaphy.2016.03.036

Cited by 50 publications

(49 citation statements)

References 30 publications

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“…In ten Rhododendron species from Tibet, cyanidin and malvidin glycosides were detected (Liu et al . ). In this work, glycosides of cyanidin, delphinidin and malvidin were also identified.…”

Section: Discussionmentioning

confidence: 97%

“…; Liu et al . ). However, Dp3Arb5G and Dp3G were identified for the first time in Rhododendron flowers.…”

Section: Discussionmentioning

confidence: 97%

“…; Liu et al . ). However, the anthocyanins detected from evergreen azaleas have not been comprehensively identified and quantified; therefore, it is difficult to compare the present results with those from previous studies individually, and it is possible that not all peaks detected were anthocyanins.…”

Section: Discussionmentioning

confidence: 97%

“…The results showed generally low total anthocyanin (TA) content and high total flavonol (TF) content, indicating co‐pigmentation effects in these species (Liu et al . ). Overall, the floral colouration and anthocyanin composition of a large number of Rhododendron species remains unexplored.…”

Section: Introductionmentioning

confidence: 97%

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Characterisation of flower colouration in 30 Rhododendron species via anthocyanin and flavonol identification and quantitative traits

Lai

Wang

et al. 2017

Plant Biol J

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Floral colour is a key reproductive character, often associated with environmental adaptation, and subject to human intervention. A large number of Rhododendron species differ widely in flower colour, providing a good model for flower colouration. The chromatic features and anthocyanin compositions of 30 species from seven subgenera were systematically analysed. The Royal Horticultural Society Colour Chart and CIE L*a*b* system were employed to describe and investigate flower colours. The UPLC-PDA/ESI-MS system was used to identify and quantify anthocyanins in petal extracts. The flower colours of 30 Rhododendron species were categorised into four groups - red, purplish pink, purple and white. Seven anthocyanins were identified and quantified in petals: delphinidin, cyanidin and malvidin 3-O-arabinoside-5-O-glucosides, cyanidin 3,5-di-O-glucoside, 3-O-galactoside and 3-O-arabinoside, and delphinidin 3-O-glucoside. The red-flowered species mainly contained cyanidin monoglycosides and had much higher total anthocyanin content than purplish pink- and purple-flowered species. Purplish pink- and purple-flowered species had similar anthocyanin types and content. The chromatic differences were significant among groups, except the purplish pink and purple groups. Statistical analysis showed that Cy3Gal and Cy3Arb are characteristic for red-flowered species, and Mv3Arb5G and Dp3Arb5G play important roles in purple colouration; their contents were major components that greatly affected the chromatic parameters. In total, 21 flavonol derivates were identified. However, total flavonol content and co-pigmentation index showed no significant difference or correlation among/with colour groups, suggesting that flavonols might not play a major role in colouration. These results enhance our knowledge of the biochemical basis of flower colouration in Rhododendron species, and provide a foundation for genetic variation studies and aid in breeding cultivars with novel flower colours.

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“…In ten Rhododendron species from Tibet, cyanidin and malvidin glycosides were detected (Liu et al . ). In this work, glycosides of cyanidin, delphinidin and malvidin were also identified.…”

Section: Discussionmentioning

confidence: 97%

“…; Liu et al . ). However, Dp3Arb5G and Dp3G were identified for the first time in Rhododendron flowers.…”

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

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Characterisation of flower colouration in 30 Rhododendron species via anthocyanin and flavonol identification and quantitative traits

Lai

Wang

et al. 2017

Plant Biol J

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“…(2016) descreveram a preparação de nanopartículas a base de quercetina, reduzindo os efeitos colaterais do Alzheimer (13). A literatura descreve a detecção de quercetina em várias plantas, utilizando diversos métodos e técnicas de análise (14)(15)(16)(17)(18)(19), dentre elas a espectroscopia de ultravioleta (16,20).…”

Section: Introductionunclassified

Uso De Espectroscopia De Ultravioleta Visível (Uv-Vis) Para a Detecção De Quercetina Em Plantas Por Meio Da Formação Do Complexo Quercetina-Alumínio

Bernardi

Nicolini

2017

Infarma

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Nine plant samples were studied from complexation reaction that occurs between aluminum from aluminum chloride (AlCl 3 ) and quercetin. The reaction shows yellow solution, which was spectroscopically evidenced by the bathochromic shift from 360-370 nm to 420-430 nm. Four samples of petals were investigated: white and magenta azalea, burnt yellow lily and dandelion; three leaf samples: aloe, orange and lime; and two peel samples: orange and lime fruits. Among the analyzed samples, only azalea (Rhododendron sp.) white and magenta petals had sufficient quercetin concentration to be detected by UV-Vis spectroscopy and analyzed by quercetin-aluminum complex formation. The interaction between aluminum and quercetin showed a stoichiometry of 2:1, with a binding constant of 3.94 ± 0.34 x 10 3 mol L -1 .

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Identification and quantification of anthocyanins, flavonoids, and phenolic acids in flowers ofRhododendron arboreumand evaluation of their antioxidant potential

Bhatt

Sendri

Swati

et al. 2022

J of Separation Science

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The current study aimed to investigate the anthocyanins, non‐anthocyanins (flavonoids and phenolic acids), and free radicals scavenging potential in the flowers of Rhododendron arboreum using ultra high performance liquid chromatography with ion mobility quadrupole time of flight tandem mass spectrometry. A total of 25 constituents including nine anthocyanins, six phenolic acids, and ten flavonoids were identified in the flower extract. The major anthocyanins identified were cyanidin‐3‐O‐β‐galactoside (1), cyanidin‐3‐O‐α‐arabinoside (4), and cyanidin‐3‐O‐rhamnoside (8), while quercetin glycosides were the main identified flavonoids in R. arboreum flowers. Additionally, ultra high performance liquid chromatography methods were developed and validated for the quantification of nine compounds (anthocyanins, flavonoid glycosides, and phenolic acids); five of them were quantified using internal standards. The extracts were analyzed for total phenolics (123.6 mg GAE/g), anthocyanin content (1.76% w/w), and evaluated for antioxidant properties against 2,2‐diphenyl‐1‐picrylhydrazyl radical (IC50: 102.06 and 96.92 μg/mL) and 2,2′‐azino‐bis (3‐ethylbenzothiazoline‐6‐sulfonic acid) radical cation (112.25 and 45.59 μM TE/g) assays. The profiling of R. arboreum for anthocyanins is reported for the first time. The findings suggest that the flowers are a promising source of bioactive constituents and could be used as functional food, antioxidants, and nutraceuticals.

show abstract

Analysis of anthocyanins and flavonols in petals of 10 Rhododendron species from the Sygera Mountains in Southeast Tibet

Cited by 50 publications

References 30 publications

Characterisation of flower colouration in 30 Rhododendron species via anthocyanin and flavonol identification and quantitative traits

Characterisation of flower colouration in 30 Rhododendron species via anthocyanin and flavonol identification and quantitative traits

Uso De Espectroscopia De Ultravioleta Visível (Uv-Vis) Para a Detecção De Quercetina Em Plantas Por Meio Da Formação Do Complexo Quercetina-Alumínio

Identification and quantification of anthocyanins, flavonoids, and phenolic acids in flowers ofRhododendron arboreumand evaluation of their antioxidant potential

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