Analysis of antibiotics by CE and their use as chiral selectors: An update

Domínguez‐Vega, Elena; Montealegre, Cristina; Marina, Marı́a Luisa

doi:10.1002/elps.201500359

Cited by 37 publications

(23 citation statements)

References 66 publications

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“…Hence, the chiral analysis and stereoisomer separations of peptides, especially peptide pharmaceuticals, are of great importance. Thanks to a high resolution power, speed and low sample and chemicals consumption, CE and CEC methods have become very powerful and popular methods for chiral analysis and stereoisomer separation in general including chiral and stereoselective separations of peptides .…”

Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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The review brings a comprehensive overview of recent developments and applications of high performance capillary and microchip electroseparation methods (zone electrophoresis, isotachophoresis, isoelectric focusing, affinity electrophoresis, electrokinetic chromatography, and electrochromatography) to analysis, microscale isolation, purification, and physicochemical and biochemical characterization of peptides in the years 2015, 2016, and ca. up to the middle of 2017. Advances in the investigation of electromigration properties of peptides and in the methodology of their analysis (sample preseparation, preconcentration and derivatization, adsorption suppression and EOF control, and detection) are described. New developments in particular CE and CEC methods are presented and several types of their applications to peptide analysis are reported: qualitative and quantitative analysis, determination in complex (bio)matrices, monitoring of chemical and enzymatical reactions and physical changes, amino acid, sequence and chiral analysis, and peptide mapping of proteins. Some micropreparative peptide separations are shown and capabilities of CE and CEC methods to provide important physicochemical characteristics of peptides are demonstrated.

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Section: Applicationsmentioning

confidence: 99%

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Kašička

2017

Electrophoresis

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“…ILs have unique performance, such as minimal vapor pressure, good thermal and chemical stability, high solubility power, also it can be designed with the composition of anions and cations. ILs have been successfully applied to CE as BGE or coating reagents of the capillary wall . Nevertheless, only few papers have reported the application of chiral ILs in CE for enantioseparation, and most of the chiral ILs were designed based on amino acid .…”

Section: Introductionmentioning

confidence: 99%

Investigation of the enantioselectivity of tetramethylammonium‐lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis

Sun

Liu

et al. 2019

Electrophoresis

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Recently, chiral ionic liquids (CILs) have received increasing interest in chiral separation by CE. Nevertheless, the lack of deep perception of the specific mechanism about CILs in CE for enantioseparation still perplexes a legion of researchers, despite the strenuous efforts. In this paper, a lactobionic acid based ionic liquid, tetramethylammonium‐lactobionate (TMA‐LA) was applied for the first time in CE to establish dual selector system with clindamycin phosphate (CP) for enantiomeric separation. Compared to single CP system or single TMA‐LA system significantly improved separations of seven tested rameric drugs (propranolol, nefopam, citalopram, chlorphenamine, metoprolol, bisoprolol, and esmolol) were observed in the dual selector systems. Several crucial parameters such as type and proportion of organic modifier, buffer composition and pH, and concentration of TMA‐LA/CP were systematically investigated to achieve satisfied enantioseparation. Meanwhile, molecular modeling was applied to demonstrate the chiral recognition mechanism of the TMA‐LA/CP dual‐selector separation system using the molecular docking software Autodock, which well supported the experimental results. The existence of TMA‐LA/CP complex may give rise to a higher discriminatory ability against the enantiomers, indicating the reason of improved separation in TMA‐LA/CP system. All the influence factors evaluated by means of Statistical Product and Service Solutions (SPSS) to research the influences on the chiral separation system.

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“…CE has proved to be one of the most effective methods for enantiomers' separation due to its high efficiency, robustness, low cost, simplicity, and diversity regarding different modes, such as ACE , MEKC , and NACE . The enantioseparation can be achieved using different types of chiral selectors, such as cyclodextrin and its derivatives , cyclofructans , crown ether , DNA , macrocyclic antibiotics , ionic liquids , chiral metal‐ion complexes , and proteins . However, when using selectors with UV absorbances, such as proteins and DNA, or when the separation is combined with mass spectrometric detection, the bad effects of selectors on detection must be considered.…”

Section: Introductionmentioning

confidence: 99%

Predefine resolution of enantiomers in partial filling capillary electrophoresis and two discontinuous function plugs coupling in‐capillary

et al. 2018

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In common partial filling CE (PF-CE), the capillary contains the selectors plug between the injection and detector end to avoid the selector going into the detector zone. To expand this method, we propose a mode of two discontinuous function plugs coupling in-capillary, named as plug-plug PF-CE (ppPF-CE). Initially, we present the method to predefine the effective length of chiral selector to meet the requirement of enantiomers' resolution, which could avoid some experimental procedures. With α-CD as a chiral selector, a satisfactory resolution of enantiomers d,l-tryptophan and d,l-tyrosine was obtained with a partial filling α-CD plug of optimal length and concentration. Subsequently, a second plug containing hydroxypropyl methylcellulose, organic solvents (acetonitrile and methanol), anionic and cationic surfactants (SDS and CTAB), and different concentrations of sodium phosphate buffer was inserted after the selector plug. Effects of plug-plug filling on enantiomers' migration and resolution are discussed. The ppPF-CE might be a new flexible mode for CE application.

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Analysis of antibiotics by CE and their use as chiral selectors: An update

Cited by 37 publications

References 66 publications

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Recent developments in capillary and microchip electroseparations of peptides (2015–mid 2017)

Investigation of the enantioselectivity of tetramethylammonium‐lactobionate chiral ionic liquid based dual selector systems toward basic drugs in capillary electrophoresis

Predefine resolution of enantiomers in partial filling capillary electrophoresis and two discontinuous function plugs coupling in‐capillary

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