Analysis of anticancer drugs using thin layer chromatography- A review

Uğur, Duygu Yeniceli; Uğur, Alper

doi:10.12991/jrp.2018.72

Cited by 3 publications

(2 citation statements)

References 51 publications

(55 reference statements)

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“…In chromatographic methods, confirmation of identity is obtained by comparing the retention times or retardation factors (TLC) of the test substance and the reference substance [1,2]. TLC combined with densitometry is also a great tool for investigating the content of biologically active substances in a drug, impurities present in the drug, but also for investigating many physicochemical properties, including lipophilicity of biologically active substances [3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Pyka-Pająk

2024

Preprint

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An easy and short-time TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyzes were performed on chromatographic plates precoated with silica gel 60F254 using the optimal mobile phase: chloroform + methanol + diethylamine in a volume ratio of 9:1:1. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopoeial method. The presented method is fast, sensitive, precise, selective, accurate and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

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Section: Introductionmentioning

confidence: 99%

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Pyka-Pająk

2024

Preprint

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“…The most commonly used methods for examining the identity of drugs are infrared absorption spectrophotometry, high-performance liquid chromatography (HPLC), thin-layer chromatography (TLC), and gas chromatography (GC) [1,2]. TLC combined with densitometry is also a great tool for investigating the content of biologically active substances in a drug and any impurities present in the drug, as well as for investigating many physicochemical properties, including the lipophilicity of biologically active substances [3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

Pyka-Pająk

2024

Processes

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A sensitive, easy-to-use, fast, and cost-effective TLC-densitometric method was developed for the separation of metronidazole, secnidazole, ornidazole, tinidazole, and 2-methyl-5-nitroimidazole and for the determination of metronidazole and tinidazole in Metronidazole Polpharma and Tinidazolum Polpharma tablets. Analyses were performed on chromatographic plates precoated with silica gel 60F254 using chloroform + methanol + diethylamine in a volume ratio of 9:1:1 as the optimal mobile phase. The method has been validated. The intraday and interday precision values for the three different concentrations ranged from 0.99% to 1.48% and 0.89% to 1.76%, and the precision values ranged from 1.13% to 2.48% and 0.95% to 2.49% for metronidazole and tinidazole, respectively. The limit of quantification (LOQ) was 0.036 and 0.066 µg/spot for metronidazole and tinidazole, respectively. The mean recovery was 103.1% and 100.6% for metronidazole and tinidazole, respectively. The content of metronidazole and tinidazole in tablets in relation to the content declared by the manufacturer was 101.3% and 99.8%, respectively. The obtained results were verified using the pharmacopeial method. The presented method is fast, sensitive, precise, selective, accurate, and robust. It allows for the analysis of several samples on one chromatography plate at the same time.

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UV Spectrophotometric Method Development and Validation for the Determination of Nirmatrelvir Antiviral Drug in Bulk and its In-house Formulation

R. Wani,

Jadhav,

Jain

et al. 2024

AJPA

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UV spectrophotometry was performed by Lab India Analytical UV 3092 using methanol as medium. The resolution process was performed in the range of 200–400nm to determine the maximum absorption (λ max). The λmax of Nirmatrelvir was found to be 210nm. Measure the absorbance of 10, 20, 30, 40, 50, and 60µg/ml drug solution and measure the maximum absorbance at 210 nm with a blank control. Then draw a graph from the concentration on the X-axis and the absorbance on the Y-axis to get a straight line. Validation parameters such as linearity, precision, specificity, LOD and LOQ, accuracy and robustness were evaluated according to ICH guidelines.

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Analysis of anticancer drugs using thin layer chromatography- A review

Cited by 3 publications

References 51 publications

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Pharmaceutical Preparations

Sensitive and Cost-Effective TLC-Densitometric Method for Determination of Metronidazole and Tinidazole in Tablets

UV Spectrophotometric Method Development and Validation for the Determination of Nirmatrelvir Antiviral Drug in Bulk and its In-house Formulation

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