Analysis of antimicrobial peptides from porcine neutrophils

Wessely-Szponder, Joanna; Majer‐Dziedzic, Barbara; Smolira, A.

doi:10.1016/j.mimet.2010.07.010

Cited by 31 publications

(44 citation statements)

References 28 publications

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“…Previous studies revealed that cathelicidins are cytotoxic against eukaryotic cells at high concentrations (Aarbiou et al, 2006). Our previous research estimated that there were no cytotoxic effects on studied cell lines in concentrations up to 20 mg/mL (Wessely-Szponder et al, 2010).…”

Section: Discussionmentioning

confidence: 89%

“…Moreover, indolicidin displays major antiendotoxic properties, whereas Bac2A is a more potent chemotactic agent than indolicidin (Bowdish et al, 2005). The PF used in our study for the stimulation of rabbit neutrophils were obtained according to the previously described method (Wessely-Szponder et al, 2010). The usefulness of these antimicrobial peptides isolated from porcine neutrophils as a therapeutic that modulates inflammatory response during and after bone graft surgical procedure has not yet been evaluated.…”

Section: Discussionmentioning

confidence: 99%

“…A PF mixture was isolated from neutrophils derived from fresh porcine blood collected with anticoagulant 3.8% citrate at an abattoir as described previously (Wessely-Szponder et al, 2010). Briefly, a crude extraction of blood neutrophils was obtained after red blood cells were lysed by the addition of 0.83% ammonium chloride.…”

Section: Isolation Of Propheninsmentioning

confidence: 99%

“…Prophenin (proline-phenylalanine-rich peptides, PF) belongs to cathelicidins, which are expressed in porcine neutrophils and exert properties such as antimicrobial activity (Zang et al, 2000;Wessely-Szponder et al, 2010). Previous reports estimated that some cathelicidins could act immunomodulatorily by increasing or diminishing immune response, including the influence on neutrophils.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Chou et al (2010) conducted experiments with the use of 2 antimicrobial peptides, magainin 2 and synthetic pexiganan acetate, to prevent implant infection in rabbit animal models. It is known that the prophenin used in earlier studies had antibacterial activity at concentrations from 20 mg/mL (Wessely-Szponder et al, 2010), but its influence on inflammatory cells has not been evaluated to date.…”

Section: Introductionmentioning

confidence: 99%

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The influence of porcine prophenin on neutrophils isolated from rabbit blood during implantation of calcium sulphate graft material into bone tissue

2012

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ABSTRACT:Immune dysfunction induced by surgical trauma may comprise either an inappropriately exaggerated inflammatory response or a profound suppression of cell-mediated immunity. Neutrophils are the leading cells in the first response to trauma. It is known that they mediate initial resistance to bacterial infection. Activated neutrophils can degranulate and release some enzymes such as elastase and myeloperoxidase (MPO). The function of elastase is, among others, to kill bacterial, whereas MPO is a specific enzyme of primary granules of neutrophils and a marker of in vivo neutrophil activation. Previous reports estimated that some cathelicidins could act to increase or diminish an innate immune response in which neutrophils participate. The aim of this study was to evaluate prophenins (PF) isolated from porcine leukocytes in respect to neutrophil activity and survival during implantation of calcium sulphate bone grafts substitution in rabbits. Obtained results pointed out that neutrophils responded to PF depending upon concentration. Thirty min from implantation of calcium sulphate graft, we observed the greatest release of elastase (57.01±0.49% of maximal release) in cultures stimulated with 10 mg/mL of PF, at 0 mg/mL was 51.15±0.23%, while after 24 h of incubation the greatest response was at a concentration of 20 mg/mL. MPO release after 30 min from surgery decreased significantly at 10 mg/mL. In higher concentrations, the inhibition was less pronounced. Moreover, we estimated that PF causes cytotoxicity in the highest concentration as well as the apoptosis of neutrophils.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Isolation Of Propheninsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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