Analysis of aqueous humour proteins in patients with retinoblastoma

Hadjistilianou, Theodora; Giglioni, Stefania; Micheli, L.; Vannoni, Davide; Brogi, Elena; Cevenini, Gabriele; Cortelazzo, Alessio; Francesco, Sonia De; Menicacci, Felice; Leoncini, Roberto

doi:10.1111/j.1442-9071.2011.02711.x

Cited by 11 publications

(12 citation statements)

References 33 publications

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“…Recently, proteomic analysis has been performed in various ocular diseases including diabetic retinopathy and cataract, which allows the simultaneous detection of a large number of proteins in specific cells, tissues or body fluids at a given time [10][11][12][13][14]. The main objective of the proteomic approach in these studies is to identify protein or peptide disease biomarkers for study of disease mechanisms, diagnosis and novel therapies.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of aqueous humor proteins associated with cataract development

Xiao

et al. 2015

Clinical Biochemistry

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Section: Introductionmentioning

confidence: 99%

Proteomic analysis of aqueous humor proteins associated with cataract development

Xiao

et al. 2015

Clinical Biochemistry

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“…To date, proteome analyses have been used in various ocular diseases, including diabetic retinopathy, glaucoma and cataracts. It allows the simultaneous identification of a large number of proteins at a given time in specific cells, tissues or body fluids, which aims to identify proteomic peptide biomarkers of disease for the diagnosis of eye diseases, and elucidating the disease mechanism for providing novel therapies (12)(13)(14)(15)(16). For example, Izzotti et al (6) investigated the association between aqueous humor (AH) protein content and the pathogenesis of open-angle glaucoma (POAG).…”

Section: Introductionmentioning

confidence: 99%

Identification of proteins in the aqueous humor associated with cataract development using iTRAQ methodology

Xiang¹,

Zhang²,

Li³

et al. 2017

Molecular Medicine Reports

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Proteins in the aqueous humor (AH) are important in the induction of cataract development. The identification of cataract-associated proteins assists in identifying patients and predisposed to the condition and improve treatment efficacy. Proteomics analysis has previously been used for identifying protein markers associated with eye diseases; however, few studies have examined the proteomic alterations in cataract development due to high myopia, glaucoma and diabetes. The present study, using the isobaric tagging for relative and absolute protein quantification methodology, aimed to examine cataract-associated proteins in the AH from patients with high myopia, glaucoma or diabetes, and controls. The results revealed that 445 proteins were identified in the AH groups, compared with the control groups, and 146, 264 and 130 proteins were differentially expressed in the three groups of patients, respectively. In addition, 44 of these proteins were determined to be cataract‑associated, and the alterations of five randomly selected proteins were confirmed using enzyme-linked immunosorbent assays. The biological functions of these 44 cataract-associated proteins were analyzed using Gen Ontology/pathways annotation, in addition to protein‑protein interaction network analysis. The results aimed to expand current knowledge of the pathophysiologic characteristics of cataract development and provided a panel of candidates for biomarkers of the disease, which may assist in further diagnosis and the monitoring of cataract development.

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“…Hadjistilianou et al, analyzed aqueous humor proteins in patients with RB and reported an increase in protein content in eyes with RB (13) . In order to investigate malignant cells, the samples were collected and a histopathological analysis was performed.…”

Section: Discussionmentioning

confidence: 99%

Phacoemulsification and foldable acrylic IOL implantation in children with treated retinoblastoma

Tartarella¹,

Britez-Colombi²,

Motono³

et al. 2012

Arq. Bras. Oftalmol.

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RESUMO ABSTRACTPurpose: To study the results of cataract surgery in children with radiation-induced cataract after treatment for retinoblastoma. Methods: Retrospective interventional case series. Six consecutive patients diagnosed with secondary cataracts due to radiation therapy for retinoblastoma. Intervention: Phacoemulsification and foldable acrylic intraocular lens implantation. Outcomes mea sured: Visual acuity, binocular indirect ophthalmoscopy and slit-lamp biomicroscopy. Aspirated lens material and aqueous humor samples were collected during surgery. Results: Six uniocular children between 3 to 5 years of age at time of surgery were studied. The mean time interval between radiotherapy and cataract diagnosis was 22.3 months. The mean follow-up after surgery was 17.2 months (range: 12 to 23 months). All eyes achieved a clear visual axis after surgery allowing monitoring of the tumor status. None developed recurrence or retinoblastoma dissemination. His topathological analysis of the aspired material showed no tumoral cells in all sam ples. All patients improved vision after cataract surgery. Conclusions: Phacoemulsification with acrylic intraocular lens implantation seems to be a safe, feasible, and effective method for the removal of radiation-induced cataracts in patients with treated retinoblastoma.

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Analysis of aqueous humour proteins in patients with retinoblastoma

Cited by 11 publications

References 33 publications

Proteomic analysis of aqueous humor proteins associated with cataract development

Proteomic analysis of aqueous humor proteins associated with cataract development

Identification of proteins in the aqueous humor associated with cataract development using iTRAQ methodology

Phacoemulsification and foldable acrylic IOL implantation in children with treated retinoblastoma

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