Analysis of Ascorbic Acid by Ion Exclusion Chromatography with Electrochemical Detection

Kim, H.-J.; Kim, Y.-K.

doi:10.1111/j.1365-2621.1988.tb09315.x

Cited by 15 publications

(3 citation statements)

References 14 publications

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“…Zielinska et al [ 38 ] used cyclic voltammetry with glassy carbon working electrode to monitor the total antioxidant capacity and flavonoid content in onions. H. J. Kim and I. K. Kim [ 39 ] evaluated ascorbic acid content (after isolation on an anion exclusion column) by amperometric detection at a Pt working electrode operating at 0.6 V (versus Ag/AgCl). The vitamin C content in apple juice has been monitored by cyclic voltammetry by means of a Pt working electrode [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Ascorbic Acid Determination in Commercial Fruit Juice Samples by Cyclic Voltammetry

Pisoschi

Danet

Kalinowski

2008

Journal of Automated Methods and Management in Chemistry

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A method was developed for assessing ascorbic acid concentration in commercial fruit juice by cyclic voltammetry. The anodic oxidation peak for ascorbic acid occurs at about 490 mV on a Pt disc working electrode (versus SCE). The influence of the potential sweep speed on the peak height was studied. The obtained calibration graph shows a linear dependence between peak height and ascorbic acid concentration in the domain (0.1–10 mmol·L−1). The equation of the calibration graph was y = 6.391x + 0.1903 (where y represents the value of intensity measured for the anodic peak height, expressed as μA and x the analyte concentration, as mmol·L−1, r2 = 0.9995, r.s.d. = 1.14%, n = 10, Cascorbic acid = 2 mmol·L−1). The developed method was applied to ascorbic acid assessment in fruit juice. The ascorbic acid content determined ranged from 0.83 to 1.67 mmol·L−1 for orange juice, from 0.58 to 1.93 mmol·L−1 for lemon juice, and from 0.46 to 1.84 mmol·L−1 for grapefruit juice. Different ascorbic acid concentrations (from standard solutions) were added to the analysed samples, the degree of recovery being comprised between 94.35% and 104%. Ascorbic acid determination results obtained by cyclic voltammetry were compared with those obtained by the volumetric method with dichlorophenol indophenol. The results obtained by the two methods were in good agreement.

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Section: Introductionmentioning

confidence: 99%

Ascorbic Acid Determination in Commercial Fruit Juice Samples by Cyclic Voltammetry

Pisoschi

Danet

Kalinowski

2008

Journal of Automated Methods and Management in Chemistry

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“…However, these methods lack the adequate selectivity to analyze vascular and nonvascular plant extracts ( , ). Specific procedures are based on the oxidation of AA in the presence of ascorbate oxidase ( − ) and on separation techniques including gas chromatography with precolumn derivatization (), reversed-phase ( − ), ion exclusion ( − ), ion pair () and ion exchange HPLC (), isotachophoresis ( , ), and capillary electroseparation ( − ).…”

Section: Introductionmentioning

confidence: 99%

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

Herrero‐Martínez¹,

Simó‐Alfonso²,

Ramis‐Ramos³

et al. 2000

Environ. Sci. Technol.

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Simultaneous determination of ascorbic acid (AA), glutathione (GSH), and oxidized glutathione (GSSG) in vascular plants has been achieved by capillary zone electrophoresis. A background electrolyte containing a phosphate buffer of pH 7 and hexadimethrin bromide for dynamic control of electroosmotic flow was used. Separation was performed in less than 5 min. The signal was simultaneously monitored at 265 and 200 nm. Dehydroascorbic acid (DHA) was determined in another aliquot of the same plant extract upon reduction with dl-homocysteine. Limits of detection of AA, GSH, and GSSG ranged from 0.2 to 0.8 μg mL-1, which corresponded from 0.04 to 0.16 mg per 100 g fresh weight of tomato, watermelon, and potato leaves. Plants exposed to a high ozone concentration (1600 μg m-3) in a laboratory fumigation system during 1, 2, 3, and 5 h showed progressive ozone-induced oxidative stress. This could be seen as a simultaneous decrease of the AA/(AA+DHA) and GSH/(GSH+GSSG) ratios; however, the decline rate was higher for AA/(AA+DHA) than for GSH/(GSH+GSSG) in plants cultivated in the field, in open-top chambers, during ca. 50 days with much lower ozone concentrations (mean concentrations, 10 and 80 μg m-3).

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“…The basic principles followed were first to measure peak parameters and then extract from these the band extracolumn contribution on the basis of hypotheses regarding either the column or the band-broadening contribution. The numerical procedure employed here to determine the peak parameters-the Edgeworth-Cramér (EC) series fitting method-not only more powerful because it is able to give peak parameters with definite precision but, in addition, is also a self-validating method, able to give accurate peak parameter values under definite conditions (10)(11)(12)(13)(14). The purpose of this study is to 0003-2700/89/0361-1489S01.50/0 © 1989 American Chemical Society…”

Section: Resultsmentioning

confidence: 99%

Determination of nitrite in drinking water and environmental samples by ion exclusion chromatography with electrochemical detection

Kim¹,

Kim²

1989

Anal. Chem.

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An extremely sensitive determination of nitrite in drinking water (tap water and underground water) and environmental samples (rain, lake water, and soil) was achieved by ion exclusion chromatography (IEC) with electrochemical (EC) detection. Potential interferences in the determination of nitrite by the standard spectrophotometric method or by the ion exchange chromatographic method with either conductivity detection or UV detection were eliminated. The detection limit was 0.1 ppb without preconcentration. No nitrite was observed from tap water or underground drinking water. The recoveries of nitrite added to tap water at 0.02, 0.1, and 1 ppm levels were between 96 and 104.5%. The average coefficient of variation was 4.7%. The recovery results were in good agreement with those obtained by the standard spectrophotometric method. Nitrite concentrations between 0.068 and 0.19 ppm were observed in rain within a week period. A greater variation, between 0.015 and 0.26 ppm, was observed in lake water. Amounts of 19.1 ppm and 0.50 ppm nitrite were observed from fertilized and unfertilized soil, respectively.

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Analysis of Ascorbic Acid by Ion Exclusion Chromatography with Electrochemical Detection

Cited by 15 publications

References 14 publications

Ascorbic Acid Determination in Commercial Fruit Juice Samples by Cyclic Voltammetry

Ascorbic Acid Determination in Commercial Fruit Juice Samples by Cyclic Voltammetry

Simultaneous Determination ofl-Ascorbic Acid, Glutathione, and Their Oxidized Forms in Ozone-Exposed Vascular Plants by Capillary Zone Electrophoresis

Determination of nitrite in drinking water and environmental samples by ion exclusion chromatography with electrochemical detection

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