Analysis of Bacterial Biofilm Formation in Patients with Malignancy Undergoing Double J Stent Indwelling and Its Influencing Factors

Abstract

To analyze the bacterial biofilm (BF) formation in patients with malignancy undergoing double J stent indwelling and its influencing factors. Methods: A total of 167 patients with malignant tumors who received double J stent indwelling in the hospital from January 2018 to January 2021 were included in the study. The urine and double J stent samples were collected for bacterial identification and observed for BF formation on the surface of the urinary catheter under a scanning electron microscope (SEM). Univariate and multivariate logistic regression analyses were used to analyze the influencing factors of BF. Results:The BF formation rate was 34.73% (58/167). The BF formation rate of positive specimens cultured in urine and double J stent was significantly higher than that of negative ones (P<0.05). Staphylococcus was the main BF bacteria in double J stent and urine culture specimens, followed by Enterococcus, Pseudomonas, Enterobacter, and Acinetobacter. Compared with the non-BF group, the number of viable bacteria in the double J stent and urine and the catheterization time in the BF group rose markedly (P<0.05). Advanced age, chemotherapy, anemia, indwelling time ≥90d, and urinary tract infection were risk factors for BF formation in patients with malignancy undergoing double J stent indwelling (P<0.05). Conclusion:There is a high rate of BF formation in patients with malignancy undergoing double J stent indwelling, with Staphylococcus as the dominant species. Treatment requires enhanced urinary catheter management and nutritional status to inhibit BF formation and lower the rate of urinary catheter-related infections.

Analysis of Characteristics, Pathogens and Drug Resistance of Urinary Tract Infection Associated with Long-Term Indwelling Double-J Stent

“…After bacteria adhere to the surface of double-J tube, they can produce polysaccharide protein complex such as fibrin, lipoprotein, polysaccharide matrix on the surface of double-J tube to form a biofilm, which is called bacterial biofilm. [8][9][10] The bacterial biofilm on the double-J tube has extremely strong drug resistance and immune evasion, which is the main reason for repeated and refractory clinical infection. [8][9][10] Previous studies have shown that long-term indwelling of double-J stent is an important risk factor for recurrent urinary tract infection.…”

“…Current research suggests that the repeated urinary tract infection caused by indwelling double-J stent is mainly caused by bacterial biofilm on the surface of double-J stent. [8][9][10] Bacteria can produce polysaccharide protein complexes, such as fibrin, lipoprotein and polysaccharide matrix on the surface of double-J stent to form a biofilm, which is called bacterial biofilm. The formation of bacterial biofilm is a defense reaction for bacteria to adapt to the external environment.…”

“…The formation of bacterial biofilm is a defense reaction for bacteria to adapt to the external environment. [8][9][10] Bacterial biofilm can prevent bacteria from killing by antibacterial agents and promote bacteria to develop drug resistance. [8][9][10] Bacterial biofilm and bacterial colony formation are important factors for the recurrence and treatment difficulty of double-J stent associated infection.…”

“…[8][9][10] Bacterial biofilm can prevent bacteria from killing by antibacterial agents and promote bacteria to develop drug resistance. [8][9][10] Bacterial biofilm and bacterial colony formation are important factors for the recurrence and treatment difficulty of double-J stent associated infection. [8][9][10][11] In view of the refractory and high recurrence of double-J stent-associated infection, it is important to explore the clinical characteristics and pathogenic characteristics of double-J stent-associated infection for the treatment of double-J stent-associated infection.…”

“…[8][9][10] Bacterial biofilm and bacterial colony formation are important factors for the recurrence and treatment difficulty of double-J stent associated infection. [8][9][10][11] In view of the refractory and high recurrence of double-J stent-associated infection, it is important to explore the clinical characteristics and pathogenic characteristics of double-J stent-associated infection for the treatment of double-J stent-associated infection. Accordingly, this study analyzed the characteristics of urinary tract infection caused by long-term indwelling double-J stent, and explored the bacterial species and sensitive drugs related to double-J stent infection, so as to provide reference for clinical intervention and treatment.…”