Analysis of Bacterial Communities in White Clover Seeds via High-Throughput Sequencing of 16S rRNA Gene

Gao, Wen; Zheng, Chunsheng; Lei, Yahong; Kuang, Weigang

doi:10.1007/s00284-018-1607-9

Cited by 6 publications

(5 citation statements)

References 40 publications

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“…Overall, it could be noted that RISA is a reliable method that can complement other used tools for the identification of the dominant microorganism to the species level. However, stronger molecular tools such as Next Generation Sequencing (NGS) are needed for the complete characterization of the microbial diversity and fingerprinting of complex microbial communities [41,42].…”

Section: Genetic Diversity and Similarities Of Microbial Populationsmentioning

confidence: 99%

Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture

Ντάϊκου

Koumelis

Kamilari

et al. 2019

International Journal of Biobased Plastics

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Section: Genetic Diversity and Similarities Of Microbial Populationsmentioning

confidence: 99%

Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture

Ντάϊκου

Koumelis

Kamilari

et al. 2019

International Journal of Biobased Plastics

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“…Another well-described example of seed transmission concerned symbiotic fungi of the genus Epichloë, that produce bioactive alkaloids, which protect plant against herbivores but ultimately impact livestock health (Gagic et al 2018). Finally, diversity surveys of seed microbial communities have detected many bacterial and fungal phyla across seeds of various plant families including Amaranthaceae (Lopez-Velasco et al 2013), Asteraceae (Leff et al 2017), Cucurbitaceae (Adam et al 2018), Brassicaceae (Links et al 2014;Rezki et al 2016;Rybakova et al 2017;Rochefort et al 2019), Fabaceae (Klaedtke et al 2016;S ánchez-L ópez 2018;Gao et al 2019), Poaceae (Links et al 2014;Yang et al 2017;Escobar Rodriguez et al 2020) and Solanaceae (Bergna et al 2018;Chen et al 2020).…”

Section: Introductionmentioning

confidence: 99%

Temporal dynamics of bacterial communities during seed development and maturation

Chesneau,

Torres-Cortes,

Briand

et al. 2020

FEMS Microbiology Ecology

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Seed microbiota acts as a starting point for the assembly of the plant microbiota and contributes to successful plant establishment. To date, the order and timing of microbial taxa immigration during seed development and maturation remained unknown. We investigated the temporal dynamics of seed bacterial communities in bean and radish. A high phylogenetic turnover was observed for both plant species with few taxa associated with all seed developmental stages. Greater heterogeneity in community structure within each stage was observed for radish. While about one-third of radish seed bacterial taxa were detected in buds, flowers and fruits, very few taxa seem to be transmitted by the floral route in bean. In the latter species, bacterial populations belonging to the P. fluorescens species complex were found either in buds, flowers and fruits or in seeds. The relative phylogenetic proximity of these bacterial populations combined with their habitat specificity led us to explore the genetic determinants involved in successful seed transmission in bean. Comparative genomic analyses of representatives bacterial strains revealed dozens of coding sequences specifically associated with seed-transmitted strains. This study provided a first glimpse on processes involved in seed microbiota assembly, which could be used for designing plant-beneficial microbial consortia.

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“…It has been reported that Bacillus is also present in other plant seeds as a dominant bacteria. In the study of different seeds, such as Medicago sativa [ 31 ], Trifolium repens L. [ 27 ], white clover [ 32 ] etc., it was found that the microorganisms carried by the seeds were diverse. This conclusion is consistent with the results of previous reports Truyens [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

“…This conclusion is consistent with the results of previous reports Truyens [ 27 ]. For the study of Achnatherum inebrians [ 33 ], Elymus sibiricus and Elymus dahuricus [ 32 ], Avena sativa L. [ 34 ], etc., it was found that the dominant bacteria of most plants were Bacillus. Through the study and analysis of culturable bacteria in the Achnatherum splendens seed belt, it was found that they also had diversity.…”

Section: Discussionmentioning

confidence: 99%

“…Smith et al [ 41 ] found that newly discovered bacteria can produce bioactive substances through the investigation of endophytic bacteria in tropical plants, which has potential significance in the fields of medicine, agriculture and industry. In 2013, Shi et al [ 32 ] studied the insecticidal activity of endophytic bacteria from A. splendens , and found that the isolates had good insecticidal activity against cotton aphids, which could be used to develop new natural insecticides. In this study, the characteristics of motility, biofilm formation and antibiotic resistance of 41 strains isolated from the seeds of A. splendens were qualitatively or quantitatively studied.…”

Section: Discussionmentioning

confidence: 99%

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Diversity and Biological Characteristics of Seed-Borne Bacteria of Achnatherum splendens

Yang,

Xie,

Chen

et al. 2024

Microorganisms

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As a high-quality plant resource for ecological restoration, Achnatherum splendens has strong adaptability and wide distribution. It is a constructive species of alkaline grassland in Northwest China. The close relationship between seed-borne bacteria and seeds causes a specific co-evolutionary effect which can enhance the tolerance of plants under various stresses. In this study, 272 bacterial isolates were isolated from the seeds of Achnatherum splendens in 6 different provinces of China. In total, 41 dominant strains were identified, and their motility, biofilm formation ability and antibiotic resistance were analyzed. The results showed that the bacteria of Achnatherum splendens belonged to 3 phyla and 14 genera, of which Firmicutes was the dominant phylum and Bacillus was the dominant genus. The motility and biofilm formation ability of the isolated strains were studied. It was found that there were six strains with a moving diameter greater than 8 cm. There were 16 strains with strong biofilm formation ability, among which Bacillus with biofilm formation ability was the most common, accounting for 37.5%. The analysis of antibiotic resistance showed that sulfonamides had stronger antibacterial ability to strains. Correlation analysis showed that the resistance of strains to aminoglycosides (kanamycin, amikacin, and gentamicin) was significantly positively correlated with their biofilm formation ability. This study provides fungal resources for improving the tolerance of plants under different stresses. In addition, this is the first report on the biological characteristics of bacteria in Achnatherum splendens.

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Analysis of Bacterial Communities in White Clover Seeds via High-Throughput Sequencing of 16S rRNA Gene

Cited by 6 publications

References 40 publications

Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture

Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture

Temporal dynamics of bacterial communities during seed development and maturation

Diversity and Biological Characteristics of Seed-Borne Bacteria of Achnatherum splendens

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