Analysis of bio-anode performance through electrochemical impedance spectroscopy

Heijne, Annemiek ter; Schaetzle, Olivier; Giménez, Sixto; Navarro, L. M.; Hamelers, H.V.M.; Fabregat‐Santiago, Francisco

doi:10.1016/j.bioelechem.2015.04.002

Cited by 49 publications

(25 citation statements)

References 22 publications

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“…Additionally, microorganisms may provide temporal charge storage within the microbial cell 15, 16, 17. From a scientific perspective, BESs may function as a platform for fundamental microbiological studies,18, 19, 20 as unique metabolic properties21 can now be studied by using a plethora of electrochemical analyses 22, 23, 24…”

Section: Introductionmentioning

confidence: 99%

“…[15][16][17] From as cientific perspective, BESs may function as ap latform for fundamentalm icrobiological studies, [18][19][20] as unique metabolic properties [21] can now be studied by using ap lethora of electrochemical analyses. [22][23][24] For all current and future foreseen applicationso fB ESs, further optimization is neededb efore they may become economically viable. [25,26] With microorganisms functioning as catalysts in BESs, their growth and activity is inextricably linked to the performance of these systems.…”

Section: Introductionmentioning

confidence: 99%

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In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Molenaar

Sleutels²,

Pereirã³

et al. 2018

ChemSusChem

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Detailed studies of microbial growth in bioelectrochemical systems (BESs) are required for their suitable design and operation. Here, we report the use of optical coherence tomography (OCT) as a tool for in situ and noninvasive quantification of biofilm growth on electrodes (bioanodes). An experimental platform is designed and described in which transparent electrodes are used to allow real‐time, 3D biofilm imaging. The accuracy and precision of the developed method is assessed by relating the OCT results to well‐established standards for biofilm quantification (chemical oxygen demand (COD) and total N content) and show high correspondence to these standards. Biofilm thickness observed by OCT ranged between 3 and 90 μm for experimental durations ranging from 1 to 24 days. This translated to growth yields between 38 and 42 mgCODbiomass gCODacetate −1 at an anode potential of −0.35 V versus Ag/AgCl. Time‐lapse observations of an experimental run performed in duplicate show high reproducibility in obtained microbial growth yield by the developed method. As such, we identify OCT as a powerful tool for conducting in‐depth characterizations of microbial growth dynamics in BESs. Additionally, the presented platform allows concomitant application of this method with various optical and electrochemical techniques.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Molenaar

Sleutels²,

Pereirã³

et al. 2018

ChemSusChem

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“…Typically after at least 1 month of operation, bioanodes converge to similar electrochemical properties and community structure regardless of the inoculum source and operational conditions such as the anode potential (Yates et al, 2012;Zhu et al, 2014;Paitier et al, 2017). Most of these studies agree that exoelectrogens with efficient pathways of extracellular electron transfer (EET), such as members of the genus Geobacter, play a pivotal role in generating current and decreasing anode charge transfer resistance (Marsili et al, 2010;ter Heijne et al, 2015).…”

Section: Introductionmentioning

confidence: 93%

Electrochemical and Microbiological Characterization of Bioanode Communities Exhibiting Variable Levels of Startup Activity

Ortiz-Medina

Call

2019

Front. Energy Res.

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Microbial electrochemical technologies (METs) require the establishment of anode biofilms to generate electrical current. The factors driving bioanode formation and their variability during startup remain unclear, leading to a lack of effective strategies to initiate larger-scale systems. Accordingly, our objective was to characterize the electrochemical properties and microbial community structure of a large set of replicate bioanodes during their first cycle of current generation. To do this, we operated eight bioanode replicates at each of two fixed electrode potentials [−0.15 V and +0.15 V vs. standard hydrogen electrode (SHE)] for one fed-batch cycle. We found that startup time decreased and maximum current generation increased at +0.15 V compared to −0.15 V, but at both potentials the bioanode replicates clustered into three distinct activity levels based on when they initiated current. Despite a large variation in current generation across the eight +0.15 V bioanodes, bioanode resistance and abundance of Geobacter species remained quite similar, differing by only 10 and 12%, respectively. At −0.15 V, current production strongly followed Geobacter species abundance and bioanode resistance, wherein the largest abundance of Geobacter was associated with the lowest charge transfer resistance. Our findings show that startup variability occurs at both applied potentials, but the underlying electrochemical and microbial factors driving variability are dependent on the applied potential.

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“…The experimental setup consisted of six flat-plate MFCs similar to earlier work [40]. Each MFC had two single flow channels (one for the cathode and one for the anode) separated by a cation exchange membrane (Fumasep FKD-PK-75, Fumatech, Braunschweig, Germany).…”

Section: Microbial Fuel Cells (Mfcs) Setupmentioning

confidence: 99%

A Thin Layer of Activated Carbon Deposited on Polyurethane Cube Leads to New Conductive Bioanode for (Plant) Microbial Fuel Cell

et al. 2020

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Large-scale implementation of (plant) microbial fuel cells is greatly limited by high electrode costs. In this work, the potential of exploiting electrochemically active self-assembled biofilms in fabricating three-dimensional bioelectrodes for (plant) microbial fuel cells with minimum use of electrode materials was studied. Three-dimensional robust bioanodes were successfully developed with inexpensive polyurethane foams (PU) and activated carbon (AC). The PU/AC electrode bases were fabricated via a water-based sorption of AC particles on the surface of the PU cubes. The electrical current was enhanced by growth of bacteria on the PU/AC bioanode while sole current collectors produced minor current. Growth and electrochemical activity of the biofilm were shown with SEM imaging and DNA sequencing of the microbial community. The electric conductivity of the PU/AC electrode enhanced over time during bioanode development. The maximum current and power density of an acetate fed MFC reached 3 mA·m−2 projected surface area of anode compartment and 22 mW·m−3 anode compartment. The field test of the Plant-MFC reached a maximum performance of 0.9 mW·m−2 plant growth area (PGA) at a current density of 5.6 mA·m−2 PGA. A paddy field test showed that the PU/AC electrode was suitable as an anode material in combination with a graphite felt cathode. Finally, this study offers insights on the role of electrochemically active biofilms as natural enhancers of the conductivity of electrodes and as transformers of inert low-cost electrode materials into living electron acceptors.

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Analysis of bio-anode performance through electrochemical impedance spectroscopy

Cited by 49 publications

References 22 publications

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

In situ Biofilm Quantification in Bioelectrochemical Systems by using Optical Coherence Tomography

Electrochemical and Microbiological Characterization of Bioanode Communities Exhibiting Variable Levels of Startup Activity

A Thin Layer of Activated Carbon Deposited on Polyurethane Cube Leads to New Conductive Bioanode for (Plant) Microbial Fuel Cell

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