Analysis of bioactive chemical compounds of Aspergillus niger by using gas chromatography-mass spectrometry and fourier-transform infrared spectroscopy

Hameed, Imad Hadi; Hamza, Lena Fadhil; Kamal, Sabreen A.

doi:10.5897/jpp2015.0354

Cited by 32 publications

(6 citation statements)

References 21 publications

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“…Also, Hansson et al (2012) reported that treatment with green algae (Ulva lactuca) extract led to an increase in the concentration of some biologically important compounds such as Tetraacetyl-d-xylonic nitriled -as in Figure 5. This compound have bioefficiency as stated in the study at Imad et al (2015). that proved the presence of the compound in the methanolic extract and has efficacy against bacteria and fungi.…”

Section: Fig 4 Phytolmentioning

confidence: 70%

The Effect of Seaweed Extracts on Chemical Composition of Tomato Plant (Solanum lycopersicum)

El‐Sheikh

Sleim²,

Abou-Elnasr³

2020

Alexandria Science Exchange Journal

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Section: Fig 4 Phytolmentioning

confidence: 70%

The Effect of Seaweed Extracts on Chemical Composition of Tomato Plant (Solanum lycopersicum)

El‐Sheikh

Sleim²,

Abou-Elnasr³

2020

Alexandria Science Exchange Journal

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“…GC-MS analysis revealed nine compounds amongst, 3-Deoxy-d-mannitol (37.34%) and d-Mannose (30.77%) were major compounds. Imad et al (2015) analysed the bioactive chemical compounds in methanol extracts of Aspergillus niger by using GC-MS analysis. The study revealed the presence of 6-Acetyl-ß-d-mannose which as a derivative of d-mannose observed in the current study.…”

Section: Discussionmentioning

confidence: 99%

Immunotoxicity of methanol extract of Entomopathogenic fungi, Aspergillus niger (OM514698) against House fly (Musca domestica) larva

Jayakumar

MOSES

2023

Preprint

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The entomopathogenic fungus obtained from insect cadaver was identified using preliminary morphological identification and species confirmation using DNA extraction by CTAB method. The entomopathogenic fungus strain was identified as Aspergillus niger (OM514698). Methanolic extract of fungal pathogen A. niger (OM514698) was tested against larval populations of House fly, Musca domestica at five different concentrations. At higher concentration (2%), 62% mortality was observed. The LC50 and LC90 values were 1.56% and 3.18% respectively. The interaction between the toxic metabolites and the housefly larva were studied based on their immune response. The immune response includes Total Haemocyte Count (THC), Differential Haemocyte Count (DHC), Phenoloxidase activity. The Total Haemocyte Count (943.7cells/mm3) decreased in comparison with control (1774.6 cells/mm3). Five different types of cells such as Prohaemocytes, Plasmatocytes, Granulocytes, Spherulocytes and Oenocytoids were observed. In the current study, the number of prohaemocytes, granulocytes, spherulocytes and oenocytoids were increased in treated than control. Phenoloxidase activity increased than control when treated with methanol extracts of A. niger (OM514698). GC-MS analysis of methanol extracts of A. niger have shown two major compounds such as 3-Deoxy-d-mannitol (37.34%) and d-Mannose (30.77%). The results specify that methanol extract from A. niger (OM514698) could be used as immunosuppressant in the management of House fly larval population.

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“…Furanacetic acid derivative (4-hexyl-2,5-dihydro dioxo furan acetic acid), similar to other furancarboxylic acid derivatives, has antibacterial and antifungal potential (Chang et al, 2020 ). Compounds having acids, esters, pyran, and furan from A. niger exhibited antibacterial activity against Pseudomonas aerogenosa, Escherichia coli, Proteus mirabilis, Staphylococcus aureus , and Klebsiella pneumonia (Imad et al, 2015 ). The compound 1-(3-Ethyloxiranyl) ethenone isolated from Cordia obliqua displayed antimicrobial properties against different microorganisms (Raj and Kumar, 2018 ).…”

Section: Discussionmentioning

confidence: 99%

“…Fatty acids and their esters (hexadecanoic acid, methyl ester, cis-9-hexadecenoic acid, hexadecanoic acid, 9 or 6-octadecenoic acid, and 10-octadecenoic acid methyl ester), undecane, dodecane, tetramethyl benzene, 2,6dimethyl undecane, 2-methyl dodecane, dimethyl octane, and trimethyl benzene majorly found. These metabolites have already been reported to have antifungal activity (Agoramoorthy et al, 2007;Imad et al, 2015;Casuga et al, 2016;Naqvi et al, 2019). The majority of the VOCs generated were fatty acids and their esters, which inserted themselves into the lipid bilayers of fungal membranes and caused an uncontrolled release of intracellular proteins and electrolytes, which finally caused the cytoplasmatic disintegration of fungal cells (Avis and Bélanger, 2001).…”

Section: Secondary Metabolites Profilingmentioning

confidence: 93%

Metabolomic profiling and its association with the bio-efficacy of Aspergillus niger strain against Fusarium wilt of guava

et al. 2023

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Bio-control agents are the best alternative to chemicals for the successful management of plant diseases. The fungus Aspergillus niger is known to produce diverse metabolites with antifungal activity, attracting researchers to exploit it as a bio-control agent for plant disease control. In the present study, 11 A. niger strains were isolated and screened for their antagonism against the guava wilt pathogen under in vitro and in planta conditions. Strains were identified morphologically and molecularly by sequencing the internal transcribed spacer (ITS), β-tubulin, and calmodulin genes. The strains were evaluated through dual culture, volatile, and non-volatile methods under an in vitro study. AN-11, AN-6, and AN-2 inhibited the test pathogen Fusarium oxysporum f. sp. psidii (FOP) at 67.16%, 64.01%, and 60.48%, respectively. An in planta study was conducted under greenhouse conditions with 6 months old air-layered guava plants (var. Allahabad Safeda) by pre- and post-inoculation of FOP. The AN-11 strain was found to be effective under both pre- and post-inoculation trials. Furthermore, gas chromatography–mass spectrometry (GC–MS) analysis was carried out to characterize the volatile compounds of the most potential strain, A. niger. The hexane soluble fraction showed the appearance of characteristic peaks of hexadecenoic acid methyl ester (4.41%), 10-octadecanoic acid methyl ester (3.79%), dodecane (3.21%), undecane (3.19%), gibepyrone A (0.15%), 3-methylundecane (0.36%), and citroflex A (0.38%). The ethyl acetate fraction of the bio-control fungi revealed the occurrence of major antifungal compounds, such as acetic acid ethyl ester (17.32%), benzopyron-4-ol (12.17%), 1,2,6-hexanetriol (7.16%), 2-propenoic acid ethanediyl ester (2.95%), 1-(3-ethyloxiranyl)-ethenone (0.98%), 6-acetyl-8-methoxy dimethyl chromene (0.96%), 4-hexyl-2,5-dihydro dioxo furan acetic acid (0.19%), and octadecanoic acid (1.11%). Furthermore, bio-control abilities could be due to hyper-parasitism, the production of secondary metabolites, and competition for sites and nutrients. Indeed, the results will enrich the existing knowledge of metabolomic information and support perspectives on the bio-control mechanism of A. niger.

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Analysis of bioactive chemical compounds of Aspergillus niger by using gas chromatography-mass spectrometry and fourier-transform infrared spectroscopy

Cited by 32 publications

References 21 publications

The Effect of Seaweed Extracts on Chemical Composition of Tomato Plant (Solanum lycopersicum)

The Effect of Seaweed Extracts on Chemical Composition of Tomato Plant (Solanum lycopersicum)

Immunotoxicity of methanol extract of Entomopathogenic fungi, Aspergillus niger (OM514698) against House fly (Musca domestica) larva

Metabolomic profiling and its association with the bio-efficacy of Aspergillus niger strain against Fusarium wilt of guava

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