Analysis of blaSHV-12-carrying Escherichia coli clones and plasmids from human, animal and food sources

Alonso, Carla Andrea; Michael, Geovana Brenner; Li, Jun; Somalo, Sergio; Simón, Carmen; Wang, Yang; Kaspar, Heike; Kadlec, Kristina; Torres, Carmén; Schwarz, Štefan

doi:10.1093/jac/dkx024

Cited by 53 publications

(41 citation statements)

References 34 publications

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“…Our results are in agreement with previous studies conducted in Spain reporting low to moderate (1.3%-10%) prevalence of ESBL/AmpC-producing E. coli genes in wild mammals [19]. In particular, in that study, hedgehogs, deer and minks were found as reservoirs of bla CMY-2 and bla SHV-12 E. coli variants [19,27]. However, in the present study new gene variants bla CTX-M-3 , bla SHV-1 , bla SHV-11 , bla SHV-28 and bla SHV-167 , are reported in wildlife.…”

Section: Discussionsupporting

confidence: 93%

“…Studies performed in the Iberian Peninsula in wildlife, have reported bla CTX-M-1 as the main ESBL gene circulating [19,26]. Additionally, bla CTX-M-14a and bla SHV-12 have also been frequently detected in E. coli from wild animals [12,27-29] with bla CTX-M-15 described in a recent study carried out in wild birds in Tunisia [17]. Interestingly, in the present study, bla CTX-M-1 and bla CTX-M-14a were never detected, whereas bla CMY-2, bla SHV- 12 and bla CTX-M-15 were frequently isolated not only in E. coli but also in K. pneumoniae and C. freundii isolates.…”

Section: Discussionmentioning

confidence: 99%

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High prevalence and diversity of Extended-Spectrum β-Lactamase and emergence of Carbapenemase producingEnterobacteriaceaespp in wildlife in Catalonia

Darwich

Vidal

Seminati

et al. 2019

Preprint

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19In wildlife, most of the studies focused on antimicrobial resistance (AMR) describe 20 Escherichia coli as the principal indicator of the selective pressure. In the present study, 21 new species of Enterobacteriaceae with a large panel of cephalosporin resistant (CR) 22 genes have been isolated from wildlife in Catalonia. A total of 307 wild animals were 23 examined to determine CR enterobacteria prevalence, AMR phenotypes and common 24 carbapenem and CR gene expression. The overall prevalence of CR-phenotype was 25 13% (40/307): 17.3% in wild mammals (18/104) and 11.5% in wild birds (22/191) 26 (p<0.01)). Hedgehogs presented the largest prevalence with 13.5% (14/104) of the 27 mammal specimens, followed by raptors with 7.3% (14/191) of the total bird specimens. 28Although CR E. coli was obtained most frequently (45%), other CR- 29Enterobacteriaceae spp like Klebsiella pneumoniae (20%), Citrobacter freundii (15%), 30 Enterobacter cloacae (5%), Proteus mirabilis (5%), Providencia spp (5%) and Serratia 31 marcescens (2.5%) were isolated. A high diversity of CR genes was identified among 32 the isolates, with 50% yielding blaCMY-2, 23% blaSHV-12, 20% blaCMY-1 and 18% 33 blaCTX-M-15. Additionally, new CR-gene variants and resistance to carbapenems 34 associated to OXA-48 were found. Most of the CR isolates, principally K. pneumoniae 35 and C. freundii, were multiresistant with co-resistance to fluoroquinolones, tetracycline, 36 sulphonamides and aminoglycosides. This study describes for the first time in wildlife a 37 high prevalence of Enterobacteriaceae spp harbouring a large variety of carbapenem 38 and CR genes frequently associated to nosocomial human infections. Implementation of 39 control measures to reduce the impact of anthropogenic pressure in the environment is 40 urgently needed. 41 In the last decades, the prevalence of opportunistic and antimicrobial resistant (AMR) 44 bacteria associated with nosocomial infections has suffered an important increase in 45 hospital settings. The overuse of antibiotics in human and veterinary medicine have led 46 to the spread of AMR pathogens, becoming a global health problem [1]. 47 Extended-spectrum β-lactamases (ESBLs) and plasmid-mediated AmpC-type β-48 lactamases (pAmpC) are the most common enzymes that confer resistance to broad-49 spectrum cephalosporins among members of the family Enterobacteriaceae. These β-50 lactamases have extensively diversified in response to the clinical use of new generation 51 drugs: cephalosporins, carbapenems and monobactams [2]. These enzymes are mostly 52 encoded by genes located in plasmids that can be horizontally transferred to different 53 bacteria genera [1]. Carbapenems are last-line beta-lactam antibiotics with the broadest 54 spectrum of activity. Unfortunately, carbapenems nowadays are commonly used in 55 hospital settings for the treatment of life-threatening infections caused by cephalosporin 56 resistant (CR) Enterobacteriaceae. However, the emergence of resistance to 57 carbapenems mediated by the produ...

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

High prevalence and diversity of Extended-Spectrum β-Lactamase and emergence of Carbapenemase producingEnterobacteriaceaespp in wildlife in Catalonia

Darwich

Vidal

Seminati

et al. 2019

Preprint

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“…In a previous study, this gene cassette was observed in the class 1 integron of Escherichia coli isolates (Alonso et al . ). However, the occurrence of the same gene cassettes in different species suggests the transferability of gene cassettes among the intra and interspecies level and the distribution of antimicrobial resistance genes among the bacteria were by horizontal gene transfer mechanism (Severino and Magalhaes ; Chang et al .…”

Section: Resultsmentioning

confidence: 97%

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Hossain

Dahanayake

Silva

et al. 2019

Letters in Applied Microbiology

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Aeromonas spp. are Gram‐negative opportunistic bacteria which have been commonly associated with fish diseases. In this study, antibiogram, antimicrobial resistance genes and integrons of 43 zebrafish‐borne Aeromonas spp. were studied. The isolates were identified as six Aeromonas species (A. veronii biovar veronii (n = 26), A. veronii biovar sobria (n = 3), A. hydrophila (n = 8), A. caviae (n = 3), A. enteropelogenes (n = 2) and A. dhakensis (n = 1)). Antibiogram of the isolates indicated that most of them were resistant to amoxicillin (100·00%), nalidixic acid (100·00%), oxytetracycline (100·00%), ampicillin (93·02%), tetracycline (74·42%), rifampicin (67·44%) and imipenem (65·15%). Multiple antimicrobial resistance (MAR) index values ranged from 0·19–0·44 to 90·70% isolates showed multidrug resistance. PCR of antimicrobial resistance genes revealed that the tetracycline resistance gene (tetA) was the most predominant (67·44%) among the isolates. The qnrS (53·49%), tetB (30·23%), tetE (30·23%), qnrB (23·26%) and aac(6’)‐Ib‐cr (4·65%) genes were also detected. Class 1 integrase (IntI1) gene was found in 46·51% of the isolates. Two types of class 1 integron gene cassette profiles (qacG‐aadA6‐qacG and drfA1) were identified. The results showed that zebrafish‐borne aeromonads can harbour different types of antimicrobial resistance genes and class 1 integrons. Significance and Impact of the Study Aeromonas spp. are important pathogens found in diverse environments. Antimicrobial resistance genes and integrons of ornamental fish‐borne Aeromonas spp. are not well studied. The antibiogram, antimicrobial resistance genes and class 1 integrons of Aeromonas spp. isolated from zebrafish were characterized for the first time in Korea. The prevalence of tetracycline resistance genes, plasmid‐mediated quinolone resistance genes and class 1 integron gene cassettes were observed among the isolates. The qacG‐aadA6‐qacG gene cassette was identified for the first time in Aeromonas spp. The results suggest that the wise use of antimicrobials is necessary for the better management of the ornamental fish.

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“…The gene bla CTX-M-14 was less frequent in Europe, but in Asia among the most common ESBL genes in poultry, companion animals, and humans (24). The ESBL gene bla SHV-12 was not frequently reported but was identified in ESBL-producing E. coli from poultry, dogs, and wild birds in Spain and Germany (39). Worldwide, the most common ESBL gene in E. coli isolates of human origin is bla CTX-M-15 , which is mainly associated with the pandemic E. coli clone O25:H4-ST131 (40).…”

Section: Clavulanic-acid Inhibited Class a Esblsmentioning

confidence: 99%

Antimicrobial Resistance inEscherichia coli

et al. 2018

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Multidrug resistance in has become a worrying issue that is increasingly observed in human but also in veterinary medicine worldwide. is intrinsically susceptible to almost all clinically relevant antimicrobial agents, but this bacterial species has a great capacity to accumulate resistance genes, mostly through horizontal gene transfer. The most problematic mechanisms in correspond to the acquisition of genes coding for extended-spectrum β-lactamases (conferring resistance to broad-spectrum cephalosporins), carbapenemases (conferring resistance to carbapenems), 16S rRNA methylases (conferring pan-resistance to aminoglycosides), plasmid-mediated quinolone resistance (PMQR) genes (conferring resistance to [fluoro]quinolones), and genes (conferring resistance to polymyxins). Although the spread of carbapenemase genes has been mainly recognized in the human sector but poorly recognized in animals, colistin resistance in seems rather to be related to the use of colistin in veterinary medicine on a global scale. For the other resistance traits, their cross-transfer between the human and animal sectors still remains controversial even though genomic investigations indicate that extended-spectrum β-lactamase producers encountered in animals are distinct from those affecting humans. In addition, of animal origin often also show resistances to other-mostly older-antimicrobial agents, including tetracyclines, phenicols, sulfonamides, trimethoprim, and fosfomycin. Plasmids, especially multiresistance plasmids, but also other mobile genetic elements, such as transposons and gene cassettes in class 1 and class 2 integrons, seem to play a major role in the dissemination of resistance genes. Of note, coselection and persistence of resistances to critically important antimicrobial agents in human medicine also occurs through the massive use of antimicrobial agents in veterinary medicine, such as tetracyclines or sulfonamides, as long as all those determinants are located on the same genetic elements.

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Analysis of blaSHV-12-carrying Escherichia coli clones and plasmids from human, animal and food sources

Abstract: Results from this study suggest that the dissemination of bla SHV-12 genes occurs by vertical (clonal) and horizontal transfer, the latter mainly mediated through IncI1 multidrug-resistance plasmids.

Cited by 53 publications

References 34 publications

High prevalence and diversity of Extended-Spectrum β-Lactamase and emergence of Carbapenemase producingEnterobacteriaceaespp in wildlife in Catalonia

High prevalence and diversity of Extended-Spectrum β-Lactamase and emergence of Carbapenemase producingEnterobacteriaceaespp in wildlife in Catalonia

Multidrug resistant Aeromonas spp. isolated from zebrafish (Danio rerio): antibiogram, antimicrobial resistance genes and class 1 integron gene cassettes

Antimicrobial Resistance inEscherichia coli

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