Analysis of blood plasma proteins in patients with Alzheimer's disease by two-dimensional electrophoresis, sequence homology and immunodetection

Abstract

Blood plasma proteins of patients with Alzheimer's disease (AD; senile dementia) and non-AD-type dementia were resolved by two-dimensional electrophoresis and identified by migration position in the electrophoresis pattern, sequence homology, and immunodetection by using antibodies. For the control experiments, blood plasma proteins of a healthy young individual and non-dementia patients were examined in a manner similar to that of the plasma samples of AD patients. In the plasma sample of the healthy young individual, more than 350 spots of silver-stained proteins were observed and among these spots, 73 spots were identified. Blood plasma proteins of the AD and non-AD-type dementia patients were compared with those of the control and non-dementia patients. In the blood plasma samples of five AD patients, three patients had apolipoprotein E4, and another patient showed apolipoprotein L and complement factor H. For the AD-related proteins apolipoprotein E, tau-1, and presenilin 2, proteins were examined by immunostaining with antibodies, in both AD and non-AD patients. Among the three samples of non-AD-type dementia patients, one was distinguishable by amyloid A proteins, and the other by haptoglobin isoforms.

Proteomics in Alzheimer's disease: insights into potential mechanisms of neurodegeneration

“…For AD research using proteomics, brain is not the only tissue that has been examined in order to elicit protein expression changes. Blood plasma samples have been examined for apolipoprotein E (ApoE), tau, and presenillin 2 in order to develop a less invasive screening process for AD (Ueno et al 2000). Cerebral spinal fluid has also been used to determine differences between control and AD patients (Davidsson et al 2002).…”

Proteomic analysis of human pleural effusion

“…Instead, a comparison between the map in Fig. 1 and that derived from human plasma reported in the literature [22,[24][25][26][27][28][29][30][31][32] is attempted. Pleural fluid is known to contain plasma filtrate derived from capillaries, so a considerable amount of plasma protein is expected to be present in the 2-DE map in Fig.…”

“…1. When such a comparison was made with the plasma 2-DE map published before [22,[24][25][26][27][28][29][30][31][32], several similar features of these two maps were readily recognized. Several abundant proteins such as albumin, haptoglobin, alpha-1-antitrypsin, alpha-1-antichymotrypsin, fibrinogen, and serotransferrin were present in both maps, judged by their similar M r and pI values.…”

“…Did these 44 pleural effusion proteins listed in Table 1 originate from plasma? The analysis of human plasma proteome have been pursued in recent years and reported in a number of papers [22,[24][25][26][27][28][29][30][31][32]. In a review article, Anderson and Anderson, achieved 289 proteins whose detection in plasma or serum is documented in the literature [22].…”