Analysis of Bronchoalveolar Lavage Cells and Fluids in Patients with Hypersensitivity Pneumonitis: Possible Role of Chemotactic Factors in the Pathogenesis of Disease

Yoshizawa, Yasuyuki; Ohdama, Shinichi; Tanoue, Masahiko; Tanaka, Motoichi; Ohtsuka, Morio; Uetake, Kenji; Hasegawa, Shizuo

doi:10.1159/000234084

Cited by 18 publications

(13 citation statements)

References 6 publications

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“…In agreement with previous reports [6], phenotypic anal yses of BAL lymphocytes (table 2) showed an increase in the percentage of CD8' cells and a decrease in CD4+ cells in SHP patients compared to those of normal subjects. In con trast, no significant differences in PBL were observed in the percentages of C D 3\ CD4 and CDS' cells between SHP patients and normal controls.…”

Section: Analyses O F Bal Cells and Subpopulations O F Tlymphocytes Isupporting

confidence: 92%

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A Compartmentalized Bias for T-Cell Receptor Vβ Usage in Summer-Type Hypersensitivity Pneumonitis

Murayama

Yoshizawa²,

Sato³

et al. 1995

Int Arch Allergy Immunol

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Several monoclonal antibodies specific to the human T-cell receptor (TCR) variable (V) region were used to examine whether T-cell oligoclonality plays a key role in the development of the T-cell-mediated lung disease, summer-type hypersensitivity pneumonitis (SHP). The usage of TCR V-regions was examined using bronchoalveolar lavage (BAL) and matched peripheral blood lymphocytes (PBL) in patients with SHP. In 18 SHP patients, the majority of lymphocytes in both BAL and PBL was CD3⁺ cells (94.2 vs. 63.9%, respectively), which was similar to that in normals (n = 7). Of CD3⁺ lymphocytes in a SHP patient, the percentage of CD4⁺ cells in BAL was reduced compared to those in paired PBL, whereas the percentage of CD8⁺ cells was significantly elevated. In contrast, the percentage of CD4⁺ cells was much higher than that of CD8⁺ cells in both BAL and PBL of normal subjects. While a majority of CD3⁺ T cells expressed TCR α/β⁺ in BAL and PBL from both SHP patients and normal controls, a marked increase of the expression of TCR γ/δ⁺ was observed in PBL in 4 out of 18 SHP patients. A markedly increased prevalence (> 3 SD) of specific TCR α/β⁺ V-region families in BAL was detected in 5 of 18 patients with SHP as compared to normal controls, whereas no specific increase was found in PBL. Increased Vβ8 was detected in 3 cases; Vβ6 was observed in 1 patient, and Vβ5 in another patient. These results demonstrate a compartmentalization of T cells with limited TCR V-region repertoires in subgroups of patients with SHP suggesting an accumulation of T-cell clones using a limited number of TCR V-region genes in the lung.

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Section: Analyses O F Bal Cells and Subpopulations O F Tlymphocytes Isupporting

confidence: 92%

“…BAL was performed as previously reported [6]. Briefly, 50 ml of physiological saline was introduced to the segmental bronchus of ei ther the right B4 or B5 by a fiberoptic bronchoscope, followed by suc tion at low pressure.…”

Section: Bronchoalveolar Lavagementioning

confidence: 99%

A Compartmentalized Bias for T-Cell Receptor Vβ Usage in Summer-Type Hypersensitivity Pneumonitis

Murayama

Yoshizawa²,

Sato³

et al. 1995

Int Arch Allergy Immunol

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“…A similar observation was also made in the pooled second and third aliquots pre viously documented [4], The finding of a covariation be tween eosinophil chemotactic activity and lymphocyte count in our BWs obtained from the majority of the pa tients suggests lymphocytes to be involved in the produc tion of the chemotactic activity. In another study on the acute stage of the disease, the neutrophil chemotactic ac tivity was related to the amount of T suppressor cells [3], The findings in one of our lavage samples were not in agreement with the positive correlation between number of lymphocytes and eosinophil chemotactic activity. In this case, the sample contained more than ten times as many lymphocytes as the others, while the eosinophil chemo tactic activity was within the normal range.…”

Section: Discussioncontrasting

confidence: 85%

“…Exposed, but asymp tomatic subjects may have increased circulating antibody levels [1] and lymphocytic alveolitis as signs of cell-medi ated response [2]. Although the mediators responsible for the genesis of the clinical disease are unknown, the pres ence of suppressor T cells and granulocyte chemotactic factors in lavage fluid [3] may suggest a causal involvement of these cells in the disease process.…”

Section: Introductionmentioning

confidence: 99%

Increased Eosinophil Chemotactic Activity in Bronchial Washes Obtained from Patients with Asymptomatic Allergic Alveolitis

Håkansson

Schmekel²

1992

Int Arch Allergy Immunol

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The chemotactic activities of eosinophils and neutrophils were measured in bronchial washes obtained from 6 symptom-free patients with previous extrinsic allergic alveolitis and 9 healthy volunteers. An increased chemotactic activity was found in the washes obtained from the patients, and the recovery of eosinophils was correlated with the eosinophil chemotactic activity, suggesting a causal relationship. Chromatography of the fluids obtained suggested that low molecular weight compounds were principally responsible for the increased chemotactic activity. A mild disturbance of the integrity of the alveolocapillary barrier could to some extent add to the explanation of the increased chemotactic activity in the washes obtained from the patients.

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“…A few of these patients, during subsequent summers, lived in different environments. BAL was performed and processed according to the method previously described [10]. Briefly.…”

Section: Subjectsmentioning

confidence: 99%

Immune responsiveness to inhaled antigens: local antibody production in the respiratory tract in health and lung diseases

Yoshizawa

Ohtani

Inoue

et al. 1995

Clinical and Experimental Immunology

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The pulmonary defence mechanism in summer type hypersensitivity pneumonitis induced by Trichosporon cutaneum was investigated. We have studied the antibody response to fungal antigens in sera and bronchoalveolar lavage (BAL) fluids from patients with summer type hypersensitivity pneumonitis, bird fancier's lung, interstitial pneumonia associated with collagen vascular disease (INT-PNE), and from normal volunteers. Antigens extracted from fungi frequently isolated from home environments were used in ELISA to detect IgG and IgA antibodies in sera and BAL fluids. The results of the present study show that antibody titre in the respiratory tract to a variety of fungi from home environments is modulated by ongoing pulmonary inflammation, and that antibody production against inhaled antigens is altered by pulmonary inflammation resulting from diverse pathogenesis. This study concludes that the preexisting pulmonary inflammatory disease alters antibody production in the respiratory tract in response to inhaled fungi, and that the type of alteration depends in part on the etiology of the preexisting disease.

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Analysis of Bronchoalveolar Lavage Cells and Fluids in Patients with Hypersensitivity Pneumonitis: Possible Role of Chemotactic Factors in the Pathogenesis of Disease

Cited by 18 publications

References 6 publications

A Compartmentalized Bias for T-Cell Receptor Vβ Usage in Summer-Type Hypersensitivity Pneumonitis

A Compartmentalized Bias for T-Cell Receptor Vβ Usage in Summer-Type Hypersensitivity Pneumonitis

Increased Eosinophil Chemotactic Activity in Bronchial Washes Obtained from Patients with Asymptomatic Allergic Alveolitis

Immune responsiveness to inhaled antigens: local antibody production in the respiratory tract in health and lung diseases

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