Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2

Maghrebi, M.; Baldoni, Elena; Lucchini, Giorgio; Vigani, Gianpiero; Valè, Giampiero; Sacchi, Gian Attilio; Nocito, F.F.

doi:10.3390/plants10040806

Cited by 12 publications

(5 citation statements)

References 46 publications

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“…Furthermore, GSH directly participates in the synthesis of PCs [54,55]. PCs form complexes with Cd that can be compartmentalized into the vacuoles; thus, PCs and other thiols play an important role in determining the sensitivity or tolerance in contrasting genotypes of a plant species [56]. Moreover, studies on the semihalophytic plant Mesembryanthemum crystallinum L. and different Cd hyperaccumulators such as Arabidopsis halleri, Thlaspi caerulescens, Solanum nigrum, and Sedum alfredii species indicate that both antioxidative enzymes and nonenzymatic antioxidants play a vital role in Cd tolerance [57][58][59].…”

Section: Discussionmentioning

confidence: 99%

The E3 Ubiquitin Ligase Gene Sl1 Is Critical for Cadmium Tolerance in Solanum lycopersicum L.

et al. 2022

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Heavy metal cadmium (Cd) at high concentrations severely disturbs plant growth and development. The E3 ubiquitin ligase involved in protein degradation is critical for plant tolerance to abiotic stress, but the role of E3 ubiquitin ligases in Cd tolerance is largely unknown in tomato. Here, we characterized an E3 ubiquitin ligase gene Sl1, which was highly expressed in roots under Cd stress in our previous study. The subcellular localization of Sl1 revealed that it was located in plasma membranes. In vitro ubiquitination assays confirmed that Sl1 had E3 ubiquitin ligase activity. Knockout of the Sl1 gene by CRISPR/Cas9 genome editing technology reduced while its overexpression increased Cd tolerance as reflected by the changes in the actual quantum efficiency of PSII photochemistry (ΦPSII) and hydrogen peroxide (H2O2) accumulation. Cd-induced increased activities of antioxidant enzymes including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione reductase (GR) were compromised in sl1 mutants but were enhanced in Sl1 overexpressing lines. Furthermore, the content of Cd in both shoots and roots increased in sl1 mutants while reduced in Sl1 overexpressing plants. Gene expression assays revealed that Sl1 regulated the transcript levels of heavy metal transport-related genes to inhibit Cd accumulation. These findings demonstrate that Sl1 plays a critical role in regulating Cd tolerance by relieving oxidative stress and resisting heavy metal transportation in tomato. The study provides a new understanding of the mechanism of plant tolerance to heavy metal stress.

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Section: Discussionmentioning

confidence: 99%

The E3 Ubiquitin Ligase Gene Sl1 Is Critical for Cadmium Tolerance in Solanum lycopersicum L.

et al. 2022

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“…In each sampling period (experimental setup B in the presence of SO 4 2– ), the shoots of four rice plants were cut at 1 cm above the roots with a microtome blade to collect, with a micropipette, the xylem sap exuded from the lower cut surface during a 90-min period ( Maghrebi et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

Sulfur Stable Isotope Discrimination in Rice: A Sulfur Isotope Mass Balance Study

et al. 2022

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The use of sulfur (S) stable isotopes to study S metabolism in plants is still limited by the relatively small number of studies. It is generally accepted that less S stable isotope discrimination occurs during sulfate (SO42–) uptake. However, S metabolism and allocation are expected to produce separations of S stable isotopes among the different plant S pools and organs. In this study, we measured the S isotope composition of the main S pools of rice plants grown under different SO42– availabilities in appropriate closed and open hydroponic-plant systems. The main results indicate that fractionation against 34S occurred during SO42– uptake. Fractionation was dependent on the amount of residual SO42– in the solution, showing a biphasic behavior related to the relative expression of two SO42– transporter genes (OsSULTR1;1 and OsSULTR1;2) in the roots. S isotope separations among S pools and organs were also observed as the result of substantial S isotope fractionations and mixing effects occurring during SO42– assimilation and plant S partitioning. Since the S stable isotope separations conserve the memory of the physiological and metabolic activities that determined them, we here underline the potential of the 32S/34S analysis for the detailed characterization of the metabolic and molecular processes involved in plant S nutrition and homeostasis.

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“…Under Cd stress conditions, CSSLqCDSL1 had a longer shoot length compared to 93-11, demonstrating tolerance to Cd toxicity. In another investigation by Maghrebi et al [79] two major rice cultivars (i.e., Capataz and Beirao) with different Cd tolerance were exposed to various Cd concentrations (0.01, 0.1, and 1 µM) and their potential capacities to sequester and transfer Cd. QTL mapping for toxic metal/metalloid stress tolerance in rice identified three QTLs associated with As, one associated with Cu and Hg, and two associated with Fe and Zn content.…”

Section: Qtl and Fine Gene Mappingmentioning

confidence: 99%

Recent Developments in Rice Molecular Breeding for Tolerance to Heavy Metal Toxicity

et al. 2023

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Heavy metal toxicity generally refers to the negative impact on the environment, humans, and other living organisms caused by exposure to heavy metals (HMs). Heavy metal poisoning is the accumulation of HMs in the soft tissues of organisms in a toxic amount. HMs bind to certain cells and prevent organs from functioning. Symptoms of HM poisoning can be life-threatening and not only cause irreversible damage to humans and other organisms; but also significantly reduce agricultural yield. Symptoms and physical examination findings associated with HM poisoning vary depending on the metal accumulated. Many HMs, such as zinc, copper, chromium, iron, and manganese, are present at extremely low levels but are essential for the functioning of plants. However, if these metals accumulate in the plants in sufficient concentrations to cause poisoning, serious damage can occur. Rice is consumed around the world as a staple food and incidents of HM pollution often occur in rice-growing areas. In many rice-producing countries, cadmium (Cd), arsenic (As), and lead (Pb) have been recognized as commonly widespread HMs contaminating rice fields worldwide. In addition to mining and irrigation activities, the use of fertilizers and pesticides has also contributed significantly to HM contamination of rice-growing soils around the world. A number of QTLs associated with HM stress signals from various intermediary molecules have been reported to activate various transcription factors (TFs). Some antioxidant enzymes have been studied which contribute to the scavenging of reactive oxygen species, ultimately leading to stress tolerance in rice. Genome engineering and advanced editing techniques have been successfully applied to rice to improve metal tolerance and reduce HM accumulation in grains. In this review article, recent developments and progress in the molecular science for the induction of HM stress tolerance, including reduced metal uptake, compartmentalized transportation, gene-regulated signaling, and reduced accumulation or diversion of HM particles to plant parts other than grains, are discussed in detail, with particular emphasis on rice.

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Analysis of Cadmium Root Retention for Two Contrasting Rice Accessions Suggests an Important Role for OsHMA2

Cited by 12 publications

References 46 publications

The E3 Ubiquitin Ligase Gene Sl1 Is Critical for Cadmium Tolerance in Solanum lycopersicum L.

The E3 Ubiquitin Ligase Gene Sl1 Is Critical for Cadmium Tolerance in Solanum lycopersicum L.

Sulfur Stable Isotope Discrimination in Rice: A Sulfur Isotope Mass Balance Study

Recent Developments in Rice Molecular Breeding for Tolerance to Heavy Metal Toxicity

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