Analysis of Cannabis sativa L. Collection accessions for cannabinoid contens and chemotype

Kyrychenko, Hanna; Laiko, Iryna; Mishchenko, S. V.

doi:10.36814/pgr.2019.25.09

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…In light of the curative properties of some non-psychotropic cannabinoids [10,11], the creation of medical cannabis (in compliance with current laws) has become relevant. The growing interest in such cannabis requires a quick solution, and therefore exploratory studies are conducted to identify valuable accessions with high contents of cannabidiol (CBD), cannabigerol (CBG), cannabichromene (CBC) and other non-psychotropic compounds [12,13]. It is known that inbreeding results both in aligned homozygous lines and in heterozygous material, and this can become a methods of selecting valuable plants and stabilizing the offspring populations [14].…”

Section: Introductionmentioning

confidence: 99%

Variability of cannabinoid contents depending on breeding methods

Laiko¹,

Mishchenko²,

Kyrychenko³

2021

СН

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Purpose and objectives. To reveal new possibilities for the expression of cannabis genotypes to obtain starting material with increased contents of non-psychotropic cannabinoids, without tetrahydrocannabinol and stabilized biological traits. Material and methods. In our study, we took Ukrainian hemp cultivars, the populations of which were distinguished by high stability of cannabinoid contents in comparison with foreign cultivars, as a basis. The study was carried out using breeding, biochemical and statistical methods: physical mutagenesis, inbreeding, individual selection, thin-layer and gas-liquid chromatography to evaluate plants for cannabinoids (HP 6890 Series Hewlett Packard chromatograph using an internal standard). Results and discussion. It was found that gamma rays at a dose of 200 Gy disrupted the genotypic relationships between cannabinoids, with accumulation of their minimum or maximum amounts. Lowering the irradiation dose to 150 Gy does not destabilize the population in terms of the cannabinoid contents. Inbreeding can be also used in breeding for both to create inbred lines that are homozygous for the ‘no cannabinoids’ trait and to identify lines with increased contents of cannabidiol, cannabigerol or cannabichromen. A lot of early-bred and modern cultivars (French, Polish, Hungarian, German) show a high population variability of the cannabinoid contents, proving that no systemic breeding was conducted to stabilize these traits in the populations of these cultivars. High homogeneity of populations in terms of the formation and accumulation of cannabinoids is only intrinsic to monoecious hemp cultivars bred by the Institute of Bast Crops of NAAS. The homozygosity of a population primarily depends on the control of targeted cross-pollination between cannabinoid-free plants using an express testing plants for these compounds prior to anthesis onset and subsequent analysis of the selected elite plants by thin-layer chromatography. Given the relevance of creating hemp varieties with therapeutic properties, breeding methods have been developed to shift the balance of ‘no cannabinoid at all’ towards a genetically determined increase in one of the cannabinoids (cannabidiol, cannabigerol, cannabichromene) combined with eliminated psychotropic activity (the THC content does not exceed 0.08%). Studies of cannabinoid accumulation revealed differences in their formation during the growing period. Based on the fact that the maximum accumulation of cannabinoids occurs during the seed ripening onset, and, in particular, their synthesis is the most intensive in perianths and small leaves, it is possible to evaluate the prospects for creating hemp cultivars with a branched diamond-shaped inflorescence, which will provide, in addition to a high content of one or another cannabinoid, an increased yield of plant biomass. Conclusions. Physical mutagenesis, inbreeding and selection are promising breeding methods for reducing psychotropic tetrahydrocannabinol, stabilizing cannabis cultivars in terms of the absence of cannabinoids, increasing the contents of cannabidiol, cannabigerol and cannabichromene gaining advantage from valuable collection accessions

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Section: Introductionmentioning

confidence: 99%

Variability of cannabinoid contents depending on breeding methods

Laiko¹,

Mishchenko²,

Kyrychenko³

2021

СН

Self Cite

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Features of populations of hemp varieties with different composition of cannabinoid compounds

Laiko,

Mishchenko,

Kyrychenko

2023

Fakt. eksp. evol. org.

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Aim. To study the biological features of populations of hemp varieties with the absence of narcotic activity – tetrahydrocannabinol (THC) not higher than 0.08 %, but with the presence of non-psychoactive components of cannabinoids: cannabidiol (CBD) and cannabichromene (CBC); cannabigerol (CBG); CBG and CBD. To identify breeding prospects for increasing the content of cannabinoids that have therapeutic properties. Methods. Samples of elite plants were analyzed for cannabinoid content by gas-liquid chromatography (GC Agilent Technologies HP 6890, Series Hewlett Packard). Results. The limits of variation of the population variability of the sign of CBD content of the Medana variety are from 0.0253 to 3.3469 %, 75 % of the plants of which contain CBD in the range of 1.0-2.2 %. Studies of the Vik 2020 hybrid population confirmed a high correlation between CBD and THC and the absence of a relationship between CBD and CBG, THC and CBG. More than 11 % of plants in the population contain CBG from 3.5849 to 4.6207 %. There are no correlations between CBD and CBG, CBD and THC in these plants. The presence of such plants indicates the possibility of further increasing the content of СBG to 8 % and above, without exceeding the THC limit in our country (0.08 %). Conclusions. Conducted research has proven that with the current permissible level of THC, the content of CBD can be increased only to 2.5-3.0 % (Medana variety) through purposeful selection. The hybrid Vik 2020 is especially valuable, because in its population unique plants with the presence of several cannabinoid compounds with a THC content of no more than 0.08 % were found. The obtained valuable breeding material of the Medana variety and the Vik 2020 hybrid is a donor for its inclusion in hybridization and further multi-vector selection in the direction of increasing of the content of CBD, CBC and CBG with the absence of THC.

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Analysis of Cannabis sativa L. Collection accessions for cannabinoid contens and chemotype

Cited by 2 publications

References 18 publications

Variability of cannabinoid contents depending on breeding methods

Variability of cannabinoid contents depending on breeding methods

Features of populations of hemp varieties with different composition of cannabinoid compounds

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