Analysis of caprine mycoplasmas and mycoplasma infections in goats using two‐dimensional electrophoresis and immunoblotting

Olsson, Birgitta; Bölske, Göran; Bergström, Katrin; Johansson, Karl‐Erik

doi:10.1002/elps.1150111016

Cited by 24 publications

(18 citation statements)

References 10 publications

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“…capricolum (5,6,8,15). The close relationship between these species has been confirmed by one-or two-dimensional polyacrylamide gel electrophoresis, which has been used to classify the organisms on the basis of their protein profiles (11,40,43,50).…”

mentioning

confidence: 97%

Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons

et al. 1996

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The so-called Mycoplasma mycoides cluster consists of six species or subspecies of mycoplasmas (Mollicutes). These species are pathogenic for ruminants and some of them are of great concern in veterinary medicine. The members of the M. mycoides cluster have two rRNA operons (rrnA and rrnB). The nucleotide sequences of the 16S rRNA genes of 10 strains, representing all of the known species and subspecies of the M. mycoides cluster, were determined by direct automated solid-phase DNA sequencing. The sequences of both rRNA operons were determined by a novel strategy involving in vitro amplification by PCR with one operon-specific primer pair and one general primer pair. Interestingly, sequence differences (polymorphisms) between the two operons were observed for all strains. Two strains of M. capricolum subsp. capripneumoniae were sequenced, and 15 polymorphisms were found in the type strain (F38) and 17 polymorphisms were found in the other strain (4/2LC). Eight polymorphisms were found in the 16S rRNA genes of the M. mycoides subsp. mycoides smallcolony type, and sequence length variations in a poly(A) region were observed in the 16S rRNA genes of the two operons of this species. Secondary-structure analysis showed that polymorphisms were present in both stem and loop regions. The nucleotide substitutions in the polymorphic sites of the stem regions often resulted in a change from a canonical to a noncanonical base pairing or vice versa. A compensatory mutation was never observed in the other nucleotide of the base pair. Phylogenetic analysis based on the 16S rRNA sequences indicated that Mycoplasma sp. strain PG50 should be included in the M. capricolum species group. Furthermore, the 16S rRNA sequences of M. mycoides subsp. capri and the M. mycoides subsp. mycoides large-colony type were 99.9% identical. We therefore suggest that these species be reclassified in a common species group (for instance, "Mycoplasma capri") distinct from the M. mycoides subsp. mycoides small-colony type, which formed an intermediate branch between the M. capricolum species group and the M. capri species group.Many species belonging to the class Mollicutes (trivial name, mycoplasmas) are pathogenic and of great economic concern in livestock production. There is one interesting group of six closely related mycoplasmas named the Mycoplasma mycoides cluster, consisting of several ruminant pathogens (13,49,50). This group comprises the following species, subspecies, or strains: M. capricolum subsp. capricolum (formerly M. capricolum), M. capricolum subsp. capripneumoniae (formerly Mycoplasma sp. strain F38), M. mycoides subsp. capri, the M. mycoides subsp. mycoides large-colony (LC) and small-colony (SC) types, and Mycoplasma sp. strain PG50.The best-known species in the M. mycoides cluster is perhaps the M. mycoides subsp. mycoides SC type, which is the causative agent of contagious bovine pleuropneumonia. This disease has been known since the 18th century, and the organism was first isolated in 1898 by Nocard and Roux (38). The M. myco...

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confidence: 97%

Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons

et al. 1996

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“…capri and M. mycoides subsp. mycoides LC seem to represent different serovars rather than different subspecies [4,15,16,18,21,24,27,30,32,35]. This inability to distinguish these two organisms has been debated by the International Committee on Systematics of Prokaryotes -Subcommittee on the Taxonomy of Mollicutes, and it was suggested that an additional comparative analysis was required to definitively put an end to the inquiry about the relationship between these two mycoplasmas.…”

Section: Discussionmentioning

confidence: 99%

“…mycoides LC is reported to cause a pattern of diseases similar to those induced by M. mycoides subsp. capri in goats, including mastitis, keratoconjunctivitis, polyarthritis, pneumonia and septi-numerical analysis of one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein patterns [4,16], by two-dimensional PAGE protein patterns [21,27], as well as by serological analysis [18]. A DNA probe based on a randomly selected genomic fragment was developed for the differentiation of the different members of the "mycoides cluster".…”

Section: Introductionmentioning

confidence: 99%

Mycoplasma mycoidessubsp.capriandMycoplasma mycoidessubsp.mycoidesLC can be grouped into a single subspecies

2006

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-Mycoplasma mycoides subsp. capri and Mycoplasma mycoides subsp. mycoides LC can be combined into one taxon on the basis of several contributions on both DNA sequence and protein analyses reported in the literature. Moreover, for the differentiation and identification of mycoplasmas of the "mycoides cluster", we investigated the rpoB gene, encoding the β-subunit of the RNA polymerase. A segment of 527 bp of the rpoB gene was amplified from 31 strains of ruminant mycoplasmas by PCR. The nucleotide sequences were determined and aligned, and accurate genetic relationships were calculated. Cluster analysis of rpoB DNA allowed species differentiation within the "mycoides cluster" and confirmed that M. mycoides subsp. capri and M. mycoides subsp. mycoides LC cannot be distinguished from each other. "Mycoplasma mycoides subsp. capri" is proposed as a common name for both subspecies Mycoplasma mycoides subsp. capri / Mycoplasma mycoides subsp. mycoides LC / ruminant mycoplasmoses / taxon / serovar

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“…Although supported by DDH studies (Askaa et al, 1978), the distinction of MmmLC and Mmc as separate subspecies has been debated for years, as numerous investigations, based on both genetic and phenotypic analyses, suggested that MmmLC was related more closely to Mmc than to MmmSC (AbuGroun et al, 1994;Leach et al, 1989;Manso-Silván et al, 2007;Olsson et al, 1990;Pettersson et al, 1996;Rodwell, 1982;Vilei et al, 2006). Mcc, represented by type strain California kid T , was recognized early to be a separate species within the M. mycoides cluster, grouping caprine mycoplasmas with characteristic biological, biochemical and serological properties (Tully et al, 1974).…”

Section: Species Differentiation In the Classmentioning

confidence: 99%

Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri

Manso-Silván

Vilei

Sachse

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

130

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Analysis of caprine mycoplasmas and mycoplasma infections in goats using two‐dimensional electrophoresis and immunoblotting

Cited by 24 publications

References 10 publications

Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons

Phylogeny of the Mycoplasma mycoides cluster as determined by sequence analysis of the 16S rRNA genes from the two rRNA operons

Mycoplasma mycoidessubsp.capriandMycoplasma mycoidessubsp.mycoidesLC can be grouped into a single subspecies

Mycoplasma leachii sp. nov. as a new species designation for Mycoplasma sp. bovine group 7 of Leach, and reclassification of Mycoplasma mycoides subsp. mycoides LC as a serovar of Mycoplasma mycoides subsp. capri

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