Analysis of CARD10 and CARD11 somatic mutations in patients with ovarian endometriosis

Abstract

Endometriosis is a complex and heterogeneous pre-malignant inflammatory disease harboring multiple gene mutations. Previous studies have suggested that caspase recruitment domain family member (CARD)10 and CARD11 mutations may exist in endometriosis. In the present study, a collection of endometriotic lesions and paired peripheral blood from 101 patients with ovarian endometriosis were obtained, and the entire coding sequences of the CARD10 and CARD11 genes were sequenced. Evolutionary conservation analysis and online prediction programs were applied to analyze the disease-causing potential of the identified mutations. A total of 4 novel somatic mutations were identified in 4 out of the 101 (4.0%) samples: 2 in-frame deletions in CARD10 (c.785_790delAGGAGA, p.K272_E273delKE; c.785_802delAGGAGAAGGAGAAGGAGA, p.K272_V277delKEPDNV) and 2 heterozygous missense mutations in CARD11 (c.49G>T, p.D17Y; c.160G>C, p.E54Q). The sample with CARD10 p.K272_E273delKE deletion was obtained from a 47-year-old patient who was also diagnosed with uterine leiomyoma, while the CARD10 p.K272_V277delKEPDNV-mutated sample was from a 43-year-old patient exhibiting a decreased blood eosinophil granulocyte ratio (0.3%) and an elevated serum creatine kinase level (314 U/l). The patient with the CARD11 p.D17Y mutation was 38 years old and exhibited an increased level of cancer antigen 125 (45.4 U/ml), while the patient with the CARD11 p.E54Q mutation was 46 years old and exhibited no other gynecological conditions. Evolutionary conservation analysis and online prediction programs suggested that these mutations may be disease-causing. In summary, 4 novel somatic mutations in the CARD10 and CARD11 genes were identified from amongst 101 cases of ovarian endometriosis for the first time, these mutations may serve active roles in the development of ovarian endometriosis.

Tetraspanin 1 promotes endometriosis leading to ovarian clear cell carcinoma

“…Activation of the oncogenic KRAS and PI3K pathways and inactivation of the tumor suppressor genes PTEN and ARID1A may be the main pathogenic mechanisms of this progression [17]. Accordingly, genetic mutations can explain their occurrence [18,19]. In previous studies, gene expression profiling has been conducted to understand the molecular mechanisms associated with the transition from endometriosis to OCCC [20,21].…”

Defining the genetic profile of endometriosis (Review)

“…CARD11 belongs to the CARD protein family, since it binds with B-cell CLL/lymphoma 10, and activates the inflammation-associated NF-κβ signaling pathway as well. Four novel somatic mutations in CARD10 and CARD11 genes detected among 101 patients with ovarian endometriosis, suggesting their role in the development of ovarian endometriosis (124).…”

CARMA3: A potential therapeutic target in non-cancer diseases

“…In addition, CARD10 rs9607469 and CARD10 rs9610775 are associated with primary open-angle glaucoma and amnestic mild cognitive impairment, respectively (88-90). Apart from CARD10 polymorphism, Hang et al were the first to identify two in-frame deletions in CARD10 among 101 cases of ovarian endometriosis, which may cause the disease (91). Luo et al used exome sequencing to identify the causative genes of two siblings, who are affected by autoimmune deficiency from a consanguineous family.…”

“…Apart from CARD10 polymorphism, Hang et al. were the first to identify two in-frame deletions in CARD10 among 101 cases of ovarian endometriosis, which may cause the disease ( 91 ). Luo et al.…”