Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability

Lizama, Catherine; Romero-Parra, Javier; Andrade, Daniel; Riveros, Felipe; Bórquez, Jorge; Ahmed, Shakeel; Venegas-Salas, Luis F; Cabalín, Carolina; Simirgiotis, Mario J.

doi:10.3390/antiox10081230

Cited by 26 publications

(26 citation statements)

References 61 publications

(77 reference statements)

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“…2). These results are in agreement with previous reports using carotenoid extracts of halophilic archaea from various origins (Flores et al 2020;Hegazy et al 2020;Hou and Cui 2018;Lizama et al 2021;Mandelli et al 2012;Squillaci et al 2017;Yatsunami et al 2014). ,490,523 705.5 673.5,619.4,579.1,540.3,487.4,457.6,439.1,394.5,315.2 2 BR 12.80 468,497,530 741.7 723.4,683.7,664.5,646.0,537.9,484.2,459.0,425.2,386.5,346.1,314.2 3 BR 14.44 468,496,530 741.3 648.5,593.5,561.8,501.4,450.8,406.6 4 BR 15.84 466,495,528 741.2 689.4,656.4,632.4,606.4,577.4,560.0,523.9,470.3,…”

Section: Antioxidant Activitysupporting

confidence: 93%

“…However, bacterioruberin was the predominant carotenoid accumulated by the three haloarchaea strains. Such dominance has also been reported in several previous studies (De La Vega et al 2016;Flores et al 2020;Hou and Cui 2018;Lizama et al 2021;Montero-Lobato et al 2018;Squillaci et al 2017;Yatsunami et al 2014). In fact, bacterioruberin is the main C 50 carotenoid component responsible for the color of the red archaea of the class Halobacteria (Giani et al 2019).…”

Section: Carotenoid Profile Of Haloarcula Sp Bt9supporting

confidence: 83%

“…The obtained IC50 values show that the antioxidant potential of the carotenoids extracts from the three haloarchaea strains studied is lower than that reported for other halophilic archaea. TeSe-41varied from 1 to 10 μg/mL (Flores et al 2020;Gómez-Villegas et al 2020;Lizama et al 2021;Mandelli et al 2012;Squillaci et al 2017). However, it is considerably greater than that of pigments produced by other microorganisms.…”

Section: Antioxidant Activitymentioning

confidence: 99%

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Characterization and biological activities of carotenoids produced by three haloarchaeal strains isolated from Algerian salt lakes

Sahli¹,

Gomri²,

Esclapez

et al. 2021

Arch Microbiol

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Halophilic archaea represent a promising natural source of carotenoids. However, little information is available about these archaeal metabolites and their biological effects. In the present work, carotenoids of strains Haloferax sp. ME16, Halogeometricum sp. ME3 and Haloarcula sp. BT9, isolated from Algerian salt lakes, were produced, extracted and identified by high-performance liquid chromatography-diode array detector and liquid chromatography-mass spectrometry. Analytical results revealed a variation in the composition depending on the strain with a predominance of bacterioruberin. The evaluation of antioxidant capacity using ABTS [(2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)] and DPPH (2,2-diphenyl-1-picrylhydrazyl) assays showed that these extracts have a strong antioxidant potential, in particular those of Haloferax sp. ME16 which displayed antioxidant power significantly higher than that of ascorbic acid used as standard. Antibacterial activity of carotenoid extracts against four human-pathogenic strains and four fish-pathogenic strains was evaluated by agar disk diffusion method. The results showed a good antibacterial activity. These findings suggest that the C 50 carotenoids from the studied strains offer promising prospects for biotechnological applications.

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Section: Antioxidant Activitysupporting

confidence: 93%

Section: Carotenoid Profile Of Haloarcula Sp Bt9supporting

confidence: 83%

Section: Antioxidant Activitymentioning

confidence: 99%

See 1 more Smart Citation

Characterization and biological activities of carotenoids produced by three haloarchaeal strains isolated from Algerian salt lakes

Sahli¹,

Gomri²,

Esclapez

et al. 2021

Arch Microbiol

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show abstract

“…Similarly, novel species under the genus Halorubrum, isolated from South Korea, produced C 50 carotenoid (bacterioruberin), having a strong antioxidant activity (Hwang et al, 2022). Likewise, carotenoids from novel Haloarcula sp., and Halorubrum sp., strains were isolated from the Atacama Desert (Lizama et al, 2021). Large-scale whole genome (n = 68) analysis suggested that haloarchaea has a wide diversity of carotenoid biosynthetic genes (Serrano et al, 2022), suggesting that haloarchaeal species could be a reservoir of carotenoid derivatives.…”

Section: Biotechnological Significance Of Haloarchaeamentioning

confidence: 99%

Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications

et al. 2023

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Marine environments and salty inland ecosystems encompass various environmental conditions, such as extremes of temperature, salinity, pH, pressure, altitude, dry conditions, and nutrient scarcity. The extremely halophilic archaea (also called haloarchaea) are a group of microorganisms requiring high salt concentrations (2–6 M NaCl) for optimal growth. Haloarchaea have different metabolic adaptations to withstand these extreme conditions. Among the adaptations, several vesicles, granules, primary and secondary metabolites are produced that are highly significant in biotechnology, such as carotenoids, halocins, enzymes, and granules of polyhydroxyalkanoates (PHAs). Among halophilic enzymes, reductases play a significant role in the textile industry and the degradation of hydrocarbon compounds. Enzymes like dehydrogenases, glycosyl hydrolases, lipases, esterases, and proteases can also be used in several industrial procedures. More recently, several studies stated that carotenoids, gas vacuoles, and liposomes produced by haloarchaea have specific applications in medicine and pharmacy. Additionally, the production of biodegradable and biocompatible polymers by haloarchaea to store carbon makes them potent candidates to be used as cell factories in the industrial production of bioplastics. Furthermore, some haloarchaeal species can synthesize nanoparticles during heavy metal detoxification, thus shedding light on a new approach to producing nanoparticles on a large scale. Recent studies also highlight that exopolysaccharides from haloarchaea can bind the SARS-CoV-2 spike protein. This review explores the potential of haloarchaea in the industry and biotechnology as cellular factories to upscale the production of diverse bioactive compounds.

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“…In vivo: not reported In vitro: antioxidant, anti-inflammatory [55]; antiviral and anti-cancer activity [65] Cholinesterase [66], cyclooxygenase-2 [67], α-glucosidase, α-amylase, and pancreatic lipase [68] inhibition Beneficial effects on sperm cell viability [69] Antimicrobial activity against pathogenic bacteria and fungi [54,70,71]…”

Section: In Vivo/in Vitro Activitymentioning

confidence: 99%

More Than Pigments: The Potential of Astaxanthin and Bacterioruberin-Based Nanomedicines

2023

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Carotenoids are natural products regulated by the food sector, currently used as feed dyes and as antioxidants in dietary supplements and composing functional foods for human consumption. Of the nearly one thousand carotenoids described to date, only retinoids, derived from beta carotene, have the status of a drug and are regulated by the pharmaceutical sector. In this review, we address a novel field: the transformation of xanthophylls, particularly the highly marketed astaxanthin and the practically unknown bacterioruberin, in therapeutic agents by altering their pharmacokinetics, biodistribution, and pharmacodynamics through their formulation as nanomedicines. The antioxidant activity of xanthophylls is mediated by routes different from those of the classical oral anti-inflammatory drugs such as corticosteroids and non-steroidal anti-inflammatory drugs (NSAIDs): remarkably, xanthophylls lack therapeutic activity but also lack toxicity. Formulated as nanomedicines, xanthophylls gain therapeutic activity by mechanisms other than increased bioavailability. Loaded into ad hoc tailored nanoparticles to protect their structure throughout storage and during gastrointestinal transit or skin penetration, xanthophylls can be targeted and delivered to selected inflamed cell groups, achieving a massive intracellular concentration after endocytosis of small doses of formulation. Most first reports showing the activities of oral and topical anti-inflammatory xanthophyll-based nanomedicines against chronic diseases such as inflammatory bowel disease, psoriasis, atopic dermatitis, and dry eye disease emerged between 2020 and 2023. Here we discuss in detail their preclinical performance, mostly targeted vesicular and polymeric nanoparticles, on cellular models and in vivo. The results, although preliminary, are auspicious enough to speculate upon their potential use for oral or topical administration in the treatment of chronic inflammatory diseases.

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Analysis of Carotenoids in Haloarchaea Species from Atacama Saline Lakes by High Resolution UHPLC-Q-Orbitrap-Mass Spectrometry: Antioxidant Potential and Biological Effect on Cell Viability

Cited by 26 publications

References 61 publications

Characterization and biological activities of carotenoids produced by three haloarchaeal strains isolated from Algerian salt lakes

Characterization and biological activities of carotenoids produced by three haloarchaeal strains isolated from Algerian salt lakes

Bioactive molecules from haloarchaea: Scope and prospects for industrial and therapeutic applications

More Than Pigments: The Potential of Astaxanthin and Bacterioruberin-Based Nanomedicines

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