Analysis of catecholamines and related substances using porous graphitic carbon as separation media in liquid chromatography–tandem mass spectrometry

Törnkvist, Anna; Sjöberg, Per J. R.; Markides, Karin E.; Bergquist, Jonas

doi:10.1016/j.jchromb.2003.11.036

Cited by 56 publications

(37 citation statements)

References 22 publications

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“…[2][3][4][5][6][7][8][9][10][11][12][13][14] These mainly include high-performance liquid chromatography (HPLC) with electrochemical detection 2 or mass spectrometry, 3 flow injection analysis (FIA) techniques, 4 electrochemical detection with various modified electrodes, 5,6 capillary electrophoresis (CE), 7 chemiluminescence (CL), 8 spectrophotometry, 9,10 and fluorescence detection. [11][12][13][14] However, most of these methods usually involve careful pretreatment steps when analyzing biological matrices.…”

mentioning

confidence: 99%

Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combined with Second-order Calibration

Xia

Zhu

et al. 2009

ANAL. SCI.

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A kinetic fluorometric method was proposed for the quantitative determination of epinephrine (EP) in human plasma samples with the aid of second-order calibration methods based on alternating trilinear decomposition (ATLD) and alternating fitting residue (AFR) algorithms. It was based on that EP could be gradually converted to a highly fluorescent intermediate product by an oxidation reaction, and further to a non-fluorescent degradation product (o-quinone). These methodologies fully exploit the second-order advantage of the employed three-way kinetic fluorescence data, allowing the concentrations of EP to be quantified even in the presence of uncalibrated interferences. The average recoveries obtained from ATLD and AFR with a factor number of 2 (N = 2) were 100.7 ± 3.3 and 100.4 ± 2.2%, respectively. In addition, elliptical joint confidence region (EJCR) tests as well as figures of merit (FOM) were employed to evaluate the accuracy of the two algorithms.

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confidence: 99%

Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combined with Second-order Calibration

Xia

Zhu

et al. 2009

ANAL. SCI.

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“…One of the recent methods of choice used noncommercial porous graphitic carbon column, but no data on Dopa concentrations measured and Dopa detection limits achieved (Törnkvist et al, 2004) were published. HPLC methods with mass spectrophotometric detection were not suitable because expensive equipment and a pre-purification step were required (Li et al, 2000;Letellier et al, 1997).…”

Section: Methods Validationmentioning

confidence: 99%

Assay of tyrosine hydroxylase based on high‐performance liquid chromatography separation and quantification of l‐dopa and l‐tyrosine

Olšovská

Novotná

Flieger

et al. 2007

Biomedical Chromatography

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An assay of L-tyrosine (Tyr) hydroxylating activity operating in lincomycin biosynthesis is described. The assay development consisted of HPLC procedure development, assessing the effect of reaction mixture components on non-enzymatic Dopa and Tyr oxidation, and sample stability evaluation. The HPLC procedure with isocratic elution and fluorescence detection was developed and validated. The method showed a wide linear range of Dopa determination of 0.125-25 micromol/L with lower limit of quantification (LLOQ) of 0.125 micromol/L, RSD of 7.2% and accuracy of 101.7%. The studied linear range of Tyr was 15.625 mmol/L to 500 mmol/L with LLOQ of 15.625 mmol/L, RSD of 1.1%, and accuracy of 98.1%. Recoveries for Dopa and Tyr were 100.66 +/- 0.89% and 94.76 +/- 0.94%, respectively. The inter- and intra-day accuracies and precisions were all within 10%. Samples of the reaction mixture were stable for at least 24 h at room temperature (RT) and 28 days at -20 degrees C. The method was tested for the enzyme activity monitoring in purified as well as crude preparations and enabled micro preparation of the enzyme product during confirmation of its identity. The influence of pH and ascorbic acid content in reaction mixture was studied with respect to non-enzymatic Tyr oxidation.

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“…Altered metabolism of tryptophan and increased synthesis of serotonin result in high 5-HIAA urinary concentrations. HPLC-MS/MS methods are in use for the simultaneous quantification of VMA, HVA, and 5-HIAA in human urine [51].…”

Section: Neurotransmittersmentioning

confidence: 99%

Metabolomics Using UPLC/HPLC‐Tandem Mass Spectrometry in Diagnosis and Research of Inherited Metabolic Diseases

Kulik

Kuilenburg

2012

Analytical Techniques for Clinical Chemistry

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Analysis of catecholamines and related substances using porous graphitic carbon as separation media in liquid chromatography–tandem mass spectrometry

Cited by 56 publications

References 22 publications

Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combined with Second-order Calibration

Quantitative Analysis of Epinephrine in Human Plasma Samples Using Kinetic Fluorometric Method Combined with Second-order Calibration

Assay of tyrosine hydroxylase based on high‐performance liquid chromatography separation and quantification of l‐dopa and l‐tyrosine

Metabolomics Using UPLC/HPLC‐Tandem Mass Spectrometry in Diagnosis and Research of Inherited Metabolic Diseases

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