Analysis of cDNA sequence, protein structure and expression of parotid secretory protein in pig

Yin, HaiFang; Fan, Baoliang; Zhao, Zhihui; Liu, Zhaoliang; Jing, Fei; Li, Ning

doi:10.1007/bf03184179

Cited by 2 publications

(1 citation statement)

References 15 publications

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“…xynBR (5'-TCACTGA-ACAGTGATGGA-3'). Then the xylanase gene and the signal peptide of porcine parotid secretory protein (Yin et al, 2003), was fused by overlap extension PCR. The fusion gene was inserted into the plasmid pcDNA6 (Invitrogen, USA) to generate recombinant plasmids p-xynA and p-xynB.…”

Section: Cloning and In Vitro Eukaryotic Expression Of Xylanase Genesmentioning

confidence: 99%

Generation of transgenic mice producing fungal xylanase in the saliva as a model for improving feed digestibility

Liu¹,

Zhang²,

Li³

et al. 2012

Afr. J. Biotechnol.

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The cell wall of animal feed grains contain a high proportion of xylan. Monogastric farm animals lack xylan-hydrolyzing enzymes in their gastro-intestinal tract. The indigestible dietary xylan impairs normal digestive function of single-stomached livestock by preventing efficient breakdown and assimilation of feed nutrients. The digestive capability of simple-stomached animals could be improved by providing them with a foreign xylanase gene for secretion of the enzyme into their digestive tract. In this study, we produced transgenic mice carrying an Aspergillus niger xylanse gene controlled by a salivary glandspecific regulatory element. The fungal xylanase is expressed specifically in the submandibular gland and secreted in the saliva of transgenic mice. The xylanase concentration in the saliva of transgenic mice reached 0.29 ± 0.03 U/ml. Digestibility of nutrients tends to increase in transgenic mice, although it does not differ significantly from that in wild-type controls. To our knowledge, this is the first demonstration of the production of fungal xylanase in the saliva of simple-stomached animals. Results from the present study encourage further investigation of employing transgenic technology to enhance the digestive capability of monogastric agriculture animals by introducing enzyme able to degrade dietary xylan into the digestive tract.

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Section: Cloning and In Vitro Eukaryotic Expression Of Xylanase Genesmentioning

confidence: 99%

Generation of transgenic mice producing fungal xylanase in the saliva as a model for improving feed digestibility

Liu¹,

Zhang²,

Li³

et al. 2012

Afr. J. Biotechnol.

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A New Age Constraint on the Onset of the Neoproterozoic Glaciations in the Yangtze Platform, South China

Zhang

Li²,

Liu³

et al. 2008

The Journal of Geology

100

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JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact support@jstor.org.. The University of Chicago Press is collaborating with JSTOR to digitize, preserve and extend access to The Journal of Geology. A B S T R A C TA sample from the top of the Banxi Group (early Neoproterozoic) in Hunan Province (South China) was dated by the SHRIMP zircon U-Pb method. The resulting age of 725 ± 10 Ma probably solves the debate between the synglacial and the preglacial correlation of the Liantuo Formation and indicates that the Liantuo Formation most likely correlates with the preglacial Banxi Group. Since the Banxi (Danzhou) Group is upwardly transitional to the glacial Jiangkou Group, the age becomes a new maximum age constraint on the onset of the Jiangkou glaciation, which therefore most likely correlates with the Sturtian glaciation.

show abstract

Analysis of cDNA sequence, protein structure and expression of parotid secretory protein in pig

Cited by 2 publications

References 15 publications

Generation of transgenic mice producing fungal xylanase in the saliva as a model for improving feed digestibility

Generation of transgenic mice producing fungal xylanase in the saliva as a model for improving feed digestibility

A New Age Constraint on the Onset of the Neoproterozoic Glaciations in the Yangtze Platform, South China

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