Analysis of changes in bacterial diversity in healthy and bacterial wilt mulberry samples using metagenomic sequencing and culture-dependent approaches

Abstract

IntroductionMulberry bacterial wilt is a serious destructive soil-borne disease caused by a complex and diverse group of pathogenic bacteria. Given that the bacterial wilt has been reported to cause a serious damage to the yield and quality of mulberry, therefore, elucidation of its main pathogenic groups is essential in improving our understanding of this disease and for the development of its potential control measures.MethodsIn this study, combined metagenomic sequencing and culture-dependent approaches were used to investigate the microbiome of healthy and bacterial wilt mulberry samples.ResultsThe results showed that the healthy samples had higher bacterial diversity compared to the diseased samples. Meanwhile, the proportion of opportunistic pathogenic and drug-resistant bacterial flora represented by Acinetobacter in the diseased samples was increased, while the proportion of beneficial bacterial flora represented by Proteobacteria was decreased. Ralstonia solanacearum species complex (RSSC), Enterobacter cloacae complex (ECC), Klebsiella pneumoniae, K. quasipneumoniae, K. michiganensis, K. oxytoca, and P. ananatis emerged as the main pathogens of the mulberry bacterial wilt.DiscussionIn conclusion, this study provides a valuable reference for further focused research on the bacterial wilt of mulberry and other plants.

Structure of Endophytes in the Root, Stem, and Leaf Tissues of Sweetpotato and Their Response to Sweetpotato Scab Disease Caused by Elsinoë batatas

“…mori). While the abundance of Proteobacteria in the diseased samples was significantly lower than that in the healthy samples, the opposite was true for Actinobacteria [28]. In addition, the richness of Proteobacteria and Actinobacteria in mulberry varieties with high and medium resistance to bacterial wilt was significantly higher than that in susceptible varieties [27].…”

Antibacterial Activity and Mechanism of Three Root Exudates from Mulberry Seedlings against Ralstonia pseudosolanacearum

“…This disease affects many important economic crops worldwide, including tomatoes, potatoes, tobacco, and bananas, among others [ 36 ]. Mulberry bacterial wilt constitutes a grave and devastating soil-borne disease, instigated by a multifaceted and diverse array of pathogenic bacteria that pose a considerable menace to global mulberry cultivation practices [ 3 , 15 ]. R. solanacearum has the ability to persist in soil for extended periods outside host plants, thereby presenting formidable obstacles to effective control measures.…”

“…Furthermore, mulberry’s high nutritional value and bioactive components make it a sought-after ingredient in human foods and in various formulations of traditional Chinese medicine, thus underlining its multifaceted importance across sectors. However, the production of mulberry is threatened by numerous diseases, with bacterial wilt caused by Rp being one of the most destructive soil-borne diseases that limits healthy production [ 3 , 4 ]. The initial outbreak of mulberry bacterial wilt was documented in 1969, originating from Shunde City within Guangdong province, China.…”