Analysis of chemical consistency and the anti-tumor activity of Huangqi-Ezhu (HQ-EZ) concentrated-granules and decoction

Abstract

Background: Traditional Chinese medicine decoction and modern concentrated-granules are two kinds of Chinese herbal medicine forms used in clinic at present. The former is extracted by traditional boiling method of a pre-mixed multi-herbal medicine according to the doctor's prescription. The latter is a mixture of extract active ingredients from a single variety of herbs by modern technology. It is not clear whether there is a difference in the content and efficacy of the active components between the two methods. Methods:The effective components of Huangqi-Ezhu (HQ-EZ) traditional decoction and concentratedgranules were determined by HPLC, and the subcutaneous transplanted tumor model of tumor-bearing mice was established to compare the anti-tumor effect. HQ-EZ traditional decoction and concentrated-granules were given respectively by continuous intragastric administration for 15 days. After the last administration the tumor tissue, liver and kidney were removed completely, and the corresponding indexes were detected.Results: Active components of concentrated-granules and traditional decoction are basically the same.Both of TCM forms have great anti-tumor effect against lung cancer, without toxify to liver and kidney. Conclusions:The two preparation methods have their own advantages in effective components, and the compatibility of HQ-EZ can inhibit the tumor growth of tumor-bearing mice, and has no liver and kidney toxicity.

Integrated Network Pharmacology and Experimental Verification to Explore the Molecular Mechanism of Hedysarum Multijugum Maxim–Curcumae Rhizoma Herb Pair for Treating Non-Small Cell Lung Cancer

“…verified seven components of HMMCR by HPLC, including Calycosin, Formononetin, Curcumenol, Astragaloside A, Astragaloside I, Calycosin-7-glucoside, and Astragaloside II. Meanwhile, HMMCR can significantly inhibit tumor growth, without toxifying the liver and kidney ( 27 ). Chengyong Xu et al.…”