Analysis of chicken anemia virus genome: evidence of intersubtype recombination

Eltahir, Yassir Mohammed; Qian, Kun; Jin, Wenjie; Qin, Aijian

doi:10.1186/1743-422x-8-512

Cited by 29 publications

(26 citation statements)

References 18 publications

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“…3), the recombinant region spanned the partial VP1 region and partial non-coding region. Studies have confirmed that CAV recombination is only found inter-group2324, but our research identified an intra-group recombination event among CAVs, which has not been previously described.…”

Section: Resultssupporting

confidence: 54%

Phylogenetic and molecular characterization of chicken anemia virus in southern China from 2011 to 2012

Zhang

Liu

et al. 2013

Sci Rep

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Chicken anemia virus (CAV) is an important pathogen that causes severe immunosuppression in young chickens. We have characterized 13 CAVs isolated from different commercial farms in southern China between 2011 and 2012. We discovered 92 variable residues compared to 37 other CAV complete genome sequences from other parts of the world listed in GenBank; these residues have not been previously observed. All of the Chinese CAV genomes that were characterized in this study had a glutamine at position 394, a hallmark of highly pathogenic CAVs. We also discovered that intra-group genetic recombination plays a role in generating genetic diversity in natural populations of CAV. The GD-J-12 isolate was a possible recombinant between GD-C-12 and GD-M-12 in the genomic region that encompassed both the coding and non-coding regions.

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Section: Resultssupporting

confidence: 54%

Phylogenetic and molecular characterization of chicken anemia virus in southern China from 2011 to 2012

Zhang

Liu

et al. 2013

Sci Rep

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“…This result indicated that different strains of CAV might coinfect a single bird or flock and that might lead to virus recombination (Ducatez et al., ; van Santen et al., ; Snoeck et al., ). Reports from China also showed that new strains of CAV could be generated by inter‐ or intragenotypic recombination, and the recombination region might span the coding and non‐coding regions (Eltahir, Qian, Jin, & Qin, ; He et al., ; Zhang et al., ). In the current study, the CAV DNA was cloned into a plasmid vector before sequencing analysis and the dominant sequence in a sample was easy to be selected for sequencing.…”

Section: Discussionmentioning

confidence: 99%

“…Further, the amino acid substitutions at positions 133 and 144 of VP1 can affect the ability of CAV spreading in cell culture (Renshaw et al., ). Moreover, CAV can undergo recombination to generate new genetic variants (Eltahir, Qian, Jin, & Qin, ; He et al., ; Zhang et al., ).…”

Section: Introductionmentioning

confidence: 99%

Epidemiology and molecular characterization of chicken anaemia virus from commercial and native chickens in Taiwan

Lin

Liu

et al. 2018

Transbound Emerg Dis

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Chicken infectious anaemia (CIA) is a disease with a highly economic impact in the poultry industry. The infected chickens are characterized by aplastic anaemia and extreme immunosuppression, followed by the increased susceptibility to secondary infectious pathogens and suboptimal immune responses for vaccination. Commercially available CIA vaccines are routinely used in the breeders in Taiwan to protect their progeny with maternal-derived antibodies. However, CIA cases still occur in the field and little is known about the genetic characteristics of Taiwanese chicken anaemia viruses (CAVs). In this study, CAV DNA was detected in 72 of 137 flocks collected during 2010-2015. Among the PCR-positive samples, the coding regions of 51 CAVs were sequenced. Phylogenetic analysis of the VP1 gene revealed that, although most of Taiwanese CAVs belonged to genotypes II and III, some isolates were clustered into a novel genotype (genotype IV). Moreover, a Taiwanese isolate in this novel genotype IV appeared to be derived from a recombination event between genotypes II and III viruses. Five Taiwanese CAV isolates were highly similar to the vaccine strains, 26P4 or Del-Ros. Taken together, these results indicate that the sequences of CAVs in Taiwan are variable, and inter-genotypic recombination had occurred between viruses of different genotypes. Moreover, vaccine-like strains might induce clinical signs of CIA in chickens. Our findings could be useful for understanding the evolution of CAVs and development of a better control strategy for CIA.

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“…Different molecular techniques such as polymerase chain reaction (PCR), hybridization assay, restriction enzyme analysis and sequencing have therefore been developed for CAV detection and characterization (Noteborn et al, 1992;Saini & Dandapat, 2009). VP1, the major and the sole structural protein of CAV with the highest variability and also liable for intergenotype and intersubtype recombination events (He et al, 2007;Eltahir et al, 2011a), is the most appropriate component of the virus for molecular characterization.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran

Nayabian

Mardani

2013

Avian Pathology

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Chicken anaemia virus (CAV) is an economically important pathogen of chickens with worldwide distribution. CAV is the causative agent of chicken anaemia disease, causing severe anaemia, lymphoid atrophy and immunosuppression in young birds. In the present study, the genetic variation of circulating CAVs in west Azerbaijan broiler farms was investigated and compared with CAVs from other countries. Extracted viral DNA from livers of chickens positive for CAV (46 out of 100) was used and a fragment of the VP1 gene 1390 base pairs in size was amplified. The purified products were subjected to restriction fragment-length polymorphism (RFLP) using HinfI endonuclease and nucleotide sequencing. Four different RFLP patterns were identified from all examined CAV DNAs. Sequence analysis of the VP1 gene of isolated CAV viruses revealed a high genetic distance (0.5 to 4.7%) among CAV isolates. Phylogenetic analysis showed that CAVs isolated from Iranian poultry farms clustered with CAVs isolated from different parts of the world. It was concluded that the circulating CAVs in broiler farms of west Azerbaijan had a high genomic variation.

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Analysis of chicken anemia virus genome: evidence of intersubtype recombination

Cited by 29 publications

References 18 publications

Phylogenetic and molecular characterization of chicken anemia virus in southern China from 2011 to 2012

Phylogenetic and molecular characterization of chicken anemia virus in southern China from 2011 to 2012

Epidemiology and molecular characterization of chicken anaemia virus from commercial and native chickens in Taiwan

Molecular characterization of the chicken anaemia viruses isolated from broiler farms of west Azerbaijan, Iran

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