Analysis of Chromatin Data Supports a Role For CD14+ Monocytes/Macrophages in Mediating Genetic Risk for Juvenile Idiopathic Arthritis

Crinzi, Elizabeth A.; Haley, Emma K.; Poppenberg, Kerry E.; Jiang, Kaiyu; Tutino, Vincent M.; Jarvis, James N.

doi:10.1101/2022.07.13.499916

Cited by 1 publication

(2 citation statements)

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“…A role of IL-6 in oJIA has been implied decades ago (42). In addition, a recent study investigating chromatin data in monocytes in JIA patients found that IL-6 binding was one of the top enriched processes (9). Peeters.…”

Section: Discussionmentioning

confidence: 99%

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Synovial Monocytes Drive the Pathogenesis in Oligoarticular Juvenile Idiopathic Arthritis via IL-6/JAK/STAT Signalling and Cell-Cell Interactions

Schmidt

Dahlberg

Berthold

et al. 2023

Preprint

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Objectives Synovial monocytes in oligoarticular juvenile idiopathic arthritis (oJIA) are polarized, but little is known of how they contribute to disease and attain their pathogenic features. The aim of this study was to investigate the role of monocytes in the pathogenesis of oJIA. Methods The function of synovial monocytes was analysed by several assays believed to reflect key pathogenic events, such as T-cell activation-, efferocytosis- and cytokine production assays through flow cytometry in untreated oJIA patients (n=33). The effect of synovial fluid on healthy monocytes was investigated through mass spectrometry, broad-spectrum phosphorylation assays and functional assays. Additional effects on monocytes were studied through co-cultures with primary fibroblast-like synoviocytes. Results The results demonstrate that synovial monocytes display functional alterations, e.g., increased ability to induce T-cell activation, increased efferocytosis and resistance to cytokine production following activation with LPS. In vitro, synovial fluid induced regulatory features in healthy monocytes through an IL-6/JAK/STAT mechanism. The magnitude of synovial IL-6 driven activation in monocytes was reflected in circulating cytokine levels. An increased ability to induce T-cell activation and markers of antigen presentation could be induced by co-culture with fibroblast-like synoviocytes. Conclusions Synovial monocytes in oJIA are functionally affected, drive chronic inflammation, and promote adaptive immune responses. This phenotype can be replicated in vitro through a combination of synovial fluid (through IL-6/JAK/STAT) and cell-cell interactions. These data support a role of monocytes in the pathogenesis of oJIA and highlight a group of patients more likely to benefit from targeting the IL-6/JAK/STAT axis to restore synovial homeostasis.

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Section: Discussionmentioning

confidence: 99%

“…T-and B-cells are thought to play a role, given the genetic relationship to MHC class II alleles and the presence of ANAs (4)(5)(6). Though, the contribution of members from the innate immune system, such as monocytes, is becoming increasingly recognized (7)(8)(9)(10).…”

Section: Introductionmentioning

confidence: 99%